TY - JOUR AU - Yantih, Novi AU - Wahyuningsih, Endah Giyah AU - Rahmat, Deni AU - Farida, Yunahara PY - 2020 TI - Development of Simple High Performance Liquid Chromatography for Analysis of Curcumin in Human Plasma In-Vitro JF - JURNAL ILMU KEFARMASIAN INDONESIA; Vol 18 No 2 (2020): JIFI DO - 10.35814/jifi.v18i2.947 KW - N2 - Curcumin has antioxidant, antiinfl ammatory, antitumor, apoptotic-inducing, and antiangiogenesis eff ects. In order to study the pharmacokinetics of curcumin, a method for analysis of curcumin in plasma levels is required. The aim of this study was developing of a simple High Performance Liquid Chromatography (HPLC) for analysis curcumin in human plasma in-vitro. The HPLC system was using isocratic technique in column reversed phase of C18 (Reliant® RP-18e column (4.6x250 mm; 5 μm)) and mobile phase of acetonitrile–acetic acid–aquabidest (60:1:39) at fl ow rate of 1.0 mL/min. Irbesartan was used as an internal standard. Detector was performed at a wavelength of 428 nm for curcumin and 270 nm for irbesartan. Linearity test shown linear results with a correlation coeffi cient (r) of 0.9970. LLOQ value was 0.0196 μg/mL with a diff erentiation value of 10.48-18.09%. The accuracy and precision of this method met requirement with a diff erentiation value and relative standard deviation of between -5.51-3.04 % and 1.04-1.86 %, respectively. Recovery of this method was 94.74-103.12%. This method provides good selectivity for the analysis curcurmin in human plasma. The developed of HPLC was a simple and valid method for analysis curcumin in human plasma. UR - http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/947