Potention Test of Antiacne by In Vitro and Ex Vivo from Ethanol Extract of Alfalfa Herbs (Medicago sativa L.)

  • Erika Indah Safitri Universitas Wahid Hasyim
  • Risha Fillah Fithria Universitas Wahid Hasyim
  • Eko Wahyu Saputro Universitas Wahid Hasyim
  • Danang Yogo Wijaya Universitas Wahid Hasyim

Abstract

Propionibacterium acnes is one of the bacteria that causes acne. Acne problems can be controlled with herbal plants, namely alfalfa herbs. The content of flavonoids in alfalfa herb is efficacious as an antiacne. This study aimed to determine the total flavonoid content and to test the potency of alfalfa herb ethanol extract (AHEE) as an antiacne in vitro and ex vivo induced by Propionibacterium acnes. Determination of total flavonoid levels using the spectrophotometer method with quercetin comparison. Test of alfalfa herbs ethanol extract by in vitro using disk diffusion method in concentration series 40%, 50%, 60%, 70%, and 80%. Meanwhile, test of extract by ex vivo using rabbits induced Propionibacterium acnes bacteria intradermally in 3 optimal concentration from in vitro test (50%, 60%, dan 70%). The parameter of test in the form of zone inhibition and percentage reduction in erythema diameter. Ethanol extract of alfalfa herbs contains total flavonoids of 2,323 mgQE/g. All ethanol extract of alfalfa herbs test concentrations had antibacterial activity against P. acnes with an average diameter of the respective iradic inhibitory area 14,60 mm; 14,89 mm; 15,56 mm; 16,52 mm; and 18,59 mm. The average percentage reduction in erythema diameter by ex vivo on ethanol extract of alfalfa herbs (50%, 60%, and 70%) were 48.70%, 48.26%, and 54.09%, respectively.

References

1. Brzuszkiewicz, E., January, W., Antje, W., Andrea, T., Jennifer, H., Hans, B. L., Mogens, K., Gerhard, G., Rolf, D., Hans, J. M., Thomas, F. M., dan Holger, B. Comparative Genomics and Transcriptomics of Propionibacterium acnes, Plos One, 2011, 6(6):1-13.

2. Tunnisa, M., Mulqie, L., dan Hajar, S., 20Uji Aktivitas Antibakteri Ekstrak Etanol Kulit Kentang (Solanum tuberosum L.) terhadap Staphylococcus aureus [skripsi]. Bandung : Fakultas Farmasi Universitas Islam Bandung; 2015. hal. 24

3. Dewi, D. N. S. Aktivitas Antibakteri Minyak Atsiri Batang Sereh (Cymbopogon citratus) terhadap Propionibacterium acnes Secara In vitro [skripsi]. Jawa Timur: Fakultas Kedokteran Universitas Jember. 2015. hal. 54

4. Karimi, E., Oskoueian, E., Oskoueian, A., Omidvar, V., Hendra, R., Nazeran, H. Insight Into the Functional and Medicinal Properties of Medicago Sativa (Alfalfa) Leaves Extract. Journal of Medicinal Plants Research. 2013. 7(7):290-297

5. Susilowati, S., Nuria, M.C., dan Budiarti., A. Identifikasi Kandungan Senyawa Kimia Ekstrak Etanol Herba Alfalfa (Medicago sativa L.), Jurnal Ilmu Farmasi dan Farmasi Klinik, 2014, 9 (2):738-741.

6. Madeswaran, A., Umamaheswari, M., Asokkumar, K., Sivashanmugam, T., Subhadradevi, V., Jagannath, P. Discovery of Potential Cyclooxygenase Inhibitors Using in Silico Docking Studies. J.Pharmacol. 2011, 7:21–27.

7. Osuntokun, O.T., Jemilaiye, T.A., and Akele, E.O. Antimicrobial Properties, Phytochemical Composition, and Phenotypic Resistance Pattern of Selected Enterophatogenic Microorganism in Ageratum conyzoides (Goat Weed) Leaf Extract, Intl. Res. J. Microbiol. 2018. 7(2):18-28

8. Doss, A., Parivuguna, V., Vijayasanthi, M., and Surendran, S. Antibacterial Evaluation and Phytochemical Analysis of Medicago sativa L. Against Some Microbial Pathogens, Indian Journal of Science and Technology, 2011, 4(5):550-552.

9. Sa’diah, S., Darusman, L.K., Triwahyuni, W., dan Batubara, I., Efektivitas Krim Anti Jerawat Kayu Secang (Caesalpinia sappan) Terhadap Prpionibacterium acnes pada Kulit Kelinci, Jurnal Ilmu Kefarmasian Indonesia, 2013, 11(2):175-181.

10. Depkes RI. Cara Pembuatan Simplisia, Jakarta : Departemen Kesehatan Republik Indonesia; 1985. p. 11.

11. Depkes RI. Materia Medika Indonesia, Jakarta : Direktorat Jendral Pengawasan Obat dan Makanan; 1995. P. 337.

12. Puspitasari, A.D., dan Wulandari, R. L. Aktivitas Antioksidan dan Penetapan Kadar Flavonoid Total Ekstrak Etil Asetat Daun Kersen (Muntingia calabura). Jurnal Pharmascience. 2017. 4(2):167-175.

13. Jangnga, I.D., Kabaya, P.P., dan Kosala, K. Uji Aktivitas Antibakteri dan Analisis Bioautografi Kromatografi Lapis Tipis Ekstrak Etanol Daun Srikaya (Annona squamosa L.) terhadap Enterococcus faecalis secara In Vitro. ODONTO Dental Journal. 2018. 5(2):104-105.

14. Samin, A., Ahmad, Bialangi, dan Salimu. Penentuan Kandungan Fenolik Total dan Aktivitas Antioksidan dari Rambut Jagung (Zea mays L.) yang Tumbuh di Daerah Gorontalo [skripsi]. Gorontalo : Fakultas Matematika dan IPA Universitas Negeri Gorontalo; 2013. hal. 57

15. Backer and Van Den Brink. Flora of Java Volume I, II, III, Netherland : Woilters Noordhoff, Groningen; 1968. p. 313.

16. Markham, K.R. Cara Mengidentifikasi Flavonoid, Bandung : Institute Teknologi Bandung Press; 1988. 5-6.

17. Ergina., Nuryanti, S., dan Puspitasari, I.D., Uji Kualitas Senyawa Metabolit Sekunder Pada Daun Palado (Agave angustifolia) Yang Diekstraksi Dengan Pelarut Air Dan Etanol. J. Akad. Kim. 2014. 3(3):169.

18. Ikalinus, R., Widyastuti, S.K., dan Setiasih, N.L.E. Skrining Fitokimia Ekstrak Etanol Kulit Batang Kelor (Moringa oleifera). Indonesia Medicus Veterinus. 2015. 4(1):76.

19. Azizah, D.N., Kumolowati, E., dan Faramayuda, F. Penetapan Kadar Flavonoid Metode AlCl3 pada Ekstrak Metanol Kulit Buah Kakao (Theobroma cacao L.). Kartika Jurnal Ilmiah Farmasi. 2014. 2(2):45-49.

20. Panche, A.N., Diwan, A.D., and Chandra, S.R. Flavonoids: an overview. Journal of Nutritional Science. 2016. 5:47.

21. Brito, R. C., Silva, D.G.N., Farias, T.C., and Ferreira, S. B. Standarization of The Safety Level of The Use of DMSO in Viability Assays in Bacterial Cells. International Conference Series on Multidiciplinary Science. 2017. 3:1-6.

22. Huda, C., Putri, A.E., dan Sari, D.W. Uji Aktivitas Antibakteri Fraksi dari Maserat Zibethinus folium terhadap Escherichia coli. Jurnal SainHealth. 2019. 3(1):7-12.

23. Mukti, D. Uji Efektivitas Antibakteri Ekstrak Etanol Buah Pare (Momordica charatia L.) Terhadap Streptococcus mutans Penyebab Karies Gigi [skripsi]. Jawa Barat : Universitas Pakuan Bogor; 2012. hal. 26.

24. Beylot, C., Auffret, N., Poli, F., Claudel, J.-P., Leccia, M.-T., Giudice, P. Del., and Dreno, B. Propionibacterium acnes: an update on its role in the pathogenesis of acne. Journal of European Academy of Dermatology Veneorology. 2013. 28 (3):271-278.

25. Angraini, L., Rostamailis., dan Minerva, P., Pengaruh Pemanfaatan Lulur Pepaya (Carica papaya L.) Terhadap Pencerahan Kulit Badan, Sumatra Barat : Fakultas Teknik Jurusan Kesejahteraan Keluarga Program Studi Pendidikan Tata Rias Dan Kecantikan. 2015. hal. 7.
Published
2021-10-31
How to Cite
SAFITRI, Erika Indah et al. Potention Test of Antiacne by In Vitro and Ex Vivo from Ethanol Extract of Alfalfa Herbs (Medicago sativa L.). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 19, n. 2, p. 254-261, oct. 2021. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1027>. Date accessed: 18 july 2024. doi: https://doi.org/10.35814/jifi.v19i2.1027.
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