DNA Barcoding of Mango Casturi (Mangifera casturi) Origin of South Borneo Based on DNA Chloroplas rbcL Gene and matK Gene
Abstract
This study aims to see the genetic diversity of the musk mango (Mangifera casturi) plant in South Kalimantan based on DNA barcode techniques using sequences from the rbcL gene and the matK gene. The plant samples used in this study were the leaves of the musk mango (Mangifera casturi) taken from eight regencies and cities in South Kalimantan Province, namely from Amuntai, Astambul, Barito Kuala, Peat, Kandanga, Babirik, Tabalong, and Tanah Laut. Isolation and DNA analysis of the musk mango plant was carried out in the research laboratory of the Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta. The analysis of the genetic diversity of the musk mango began with the stage of amplifying genomic DNA (gDNA) using rbcL and matK primers. The results of the DNA isolation samples showed a high concentration level of 17-91 ng/µL with a purity of 1.72 -1.92 ng/µL. When PCR was carried out using rbcL and matK primers, from 8 areas in South Kalimantan, 5 samples (Babirik, Tabalong, Kandangan, Amuntai, Batola, Peat, and Tanah Laut) were amplified with rbcL primers and 6 samples (Tanah Laut, Babirik, Tabalong , Amuntai, Peat, and Kandangan) amplified with matK primer. The relationship between the musk mango in South Kalimantan can be seen from the phylogenetic tree.
References
2. Geffen E, Luikart G, Waples RS. Impacts of modern molecular genetic techniques on conservation biology. Key topics in conservation biology. 2007:46.
3. Mayer F, Dietz C, Kiefer A. Molecular species identification boosts bat diversity. Frontiers in zoology. 2007 Dec;4(1):1-5.
4. Hidayat T, Pancoro A, Kusumawaty D, Eiadthong W. Development matK gene as DNA barcode to assess evolutionary relationship of important tropical forest tree genus Mangifera (Anacardiaceae) in Indonesia and Thailand. Jurnal Teknologi. 2012 Sep 15;59(1): 17-20.
5. Pierce BA. Genetiks: A Conceptual Approach. New York: W. H. Freeman and Company. 2002.
6. Suparman. Desain Primer PCR In Silico untuk Amplifikasi Gen rbcL pada Genus Mangifera. Jurnal Bioedukasi. 2013; 2(1):163-170.
7. Teletchea F. Molecular identification methods of fish species: reassessment and possible applications. Reviews in Fish Biology and Fisheries. 2009 Sep;19(3):265-93.
8. Chen X, Liao B, Song J, Pang X, Han J, Chen S. A fast SNP identification and analysis of intraspecific variation in the medicinal Panax species based on DNA barcoding. Gene. 2013 Nov 1;530(1):39-43.
9. Sogandi S, Riyanto J. Investigating the anti-acne potential of endophytic bacterial extracts isolated from Mangifera casturi in indigenous South Borneo, Indonesia. Journal of Agriculture and Applied Biology. 2020 Dec 24;1(2):54-63.
10. Erwina J, Fitmawati, Nery S. Analisis Filogenetik Mangifera odorata Sumatera Tengah dan Kerabatnya Menggunakan Gen rbcL. Jurnal Riau Biologia. 2016;1(2): 155 -159
11. Fattah YR, Kamu VS, Runtuwene MR, Momuat LI. Identifikasi barcode tumbuhan gedi merah (Abelmoschus manihot L. medik) dan gedi hijau (Abelmoschus moschatus) berdasarkan gen matK. Jurnal MIPA. 2014;3(2):120-4.
12. Sogandi S, Triandriani W, Saputri D, Suhendar U. Antioxidant Activity of Endophytic Bacterial Extract Isolated from Clove Leaf (Syzygium aromaticum L.). Journal of Agriculture and Applied Biology. 2020 Jun 5;1(1):9-17.
13. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular biology and evolution. 2011 Oct 1;28(10):2731-9.
14. Cheng T, Xu C, Lei L, Li C, Zhang Y, Zhou S. Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity. Molecular ecology resources. 2016 Jan;16(1):138-49.
15. Ali MA, Gyulai G, Hidvegi N, Kerti B, Al Hemaid FM, Pandey AK, Lee J. The changing epitome of species identification–DNA barcoding. Saudi journal of biological sciences. 2014 Jul 1;21(3):204-31.
16. Fitmawati F, Swita A, Sofyanti N, Herman H. Analisis Kekerabatan Morfologi Mangifera Dari Sumatera Tengah. Floribunda. 2013;4(7).
17. Priyono, Putranto RA. Molecular markers and their application for DNA fingerprinting and genetic diversity studies in Coffea species. Menara Perkebunan. 2014; 82: 39-50.
18. Arif IA, Khan HA, Bahkali AH, Al Homaidan AA, Al Farhan AH, Al Sadoon M, Shobrak M. DNA marker technology for wildlife conservation. Saudi journal of biological sciences. 2011 Jul 1;18(3):219-25.
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