The Effectiveness of Babadotan Leaves Aqueous Exctract and its Combination with Pregabalin as Anti Neuropathic-Pain and Acute Toxicity Testing

  • Yedy Purwandi Sukmawan Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada
  • Hendy Suhendy
  • Maritsa Nurfatwa


Neuropathic pain is a condition that is difficult to treated and impaired quality of life. The objective of the study was to determine the anti-neuropathic pain effectivity of Ageratum conyzoides aqueous extract and the acute toxicity study. We had 7 groups (normal, negative, pregabalin, ageratum. conyzoides-I (AC-I) 50mg/kg BW, A. conyzoides-II (AC-II) 100mg/kg BW, combination of pregabalin and AC-II), combination of AC-II and naloxone). Each group contain 5 animals (mice). All groups was induced with pyridoxine 400 mg/kg BW i.p for 14 days to cause neuropathy and followed by hyperalgesia, allodynia, and wire-hang test. In the acute toxicity study, we had 1 group and contain 5 animals (rats).  The results of the hyperalgesia and allodynia study showed the effect of AC-II was better than AC-I. The combination of pregabalin and AC-II was better than AC-II as monotherapy (p<0.05). However, AC II activity was abolished when combined with naloxone. In wire hang test demonstrated no difference between falling score (p: 0.053) and reaching score (0.903), that showed AC-II wasn’t impaired the motoric system. No death or toxicity symptoms were observed for 14 days in the acute toxicity study. A. conyzoides aqueous extract is potential for anti-neuropathic pain..


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How to Cite
SUKMAWAN, Yedy Purwandi; SUHENDY, Hendy; NURFATWA, Maritsa. The Effectiveness of Babadotan Leaves Aqueous Exctract and its Combination with Pregabalin as Anti Neuropathic-Pain and Acute Toxicity Testing. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 19, n. 2, p. 184-188, nov. 2021. ISSN 2614-6495. Available at: <>. Date accessed: 27 sep. 2023. doi: