The Effect of Simplex Powder n-Hexane Purification on Asiaticoside Content, Free Radical Scavenging and Total Phenolic Content of Gotu Kola (Centella asiatica (L.) Urban) Ethanolic Extract

  • AGATHA BUDI SUSIANA LESTARI UNIVERSITAS GADJAH MADA
  • ACHMAD FUDHOLI UNIVERSITAS GADJAH MADA
  • AKHMAD KHARIS NUGROHO UNIVERSITAS GADJAH MADA
  • ERNA PRAWITA SETYOWATI UNIVERSITAS GADJAH MADA

Abstract

It has been proven that gotu kola (Centella asiatica (L.) Urban) have an antioxidant activity that make it possible to use in preventing degenerative illness. The aims of this study are to find the effect of n-hexane purification before maceration on asiaticoside content, free radical scavenging activity and total phenolic content of gotu kola ethanolic extract. The gotu kola extract was made by maceration process using the mix of ethanol and aquadest as solvents in different proportion, which are 100%:0% (E100), 75%:25% (E75), 50%:50% (E50), 25%:75% (E25), and 0%:100% (E0). The determination of asiaticoside content in gotu kola ethanolic extract was measured using Thin Layer Chromatography (TLC) Densitometry method, free radical scavenging activity was measured using diphenylpicryl hydrazyl (DPPH) and defined by parameter of IC , and total phenolic content was defined by equivalency of galic acid using Folin-Ciocalteau. The results show that the hexane purification would increasing total phenolic content significantly in ethanolic extract which extracted with solvents in proportion of ethanol:aquades 75%:25% (E75), 50%:50% (E50), and 25%:75% (E25), but not significant in decreasing of IC . Statistically, n-hexane purification was also not significant to increasing asiaticoside content in gotu kola ethanolic extract.  

References

1. Zheng C, Qin L. Chemical components of Centella asiatica and their bioactivities. Zhong Xi Yi Jie He Xue Bao. 2007. 5(3):348–51.
2. H amid A , S hah ZM , Mus e R, Mohamed S . Characterisation of antioxidative activities of various extracts of Centella asiatica (L) Urban. Food Chem. 2002. 77(4):465–9.
3. Departemen Kesehatan Republik Indonesia. Sediaan Galenik. Jakarta: Departemen Kesehatan Republik Indonesia; 1986. 16-7.
4. Pramono S, Ajiastuti D. Standardisasi ekstrak herba pegagan (Centella asiatica (L.) Urban) berdasarkan kadar asiatikosida secara KLT-densitometri. Majalah Farmasi Indonesia. 2004. 15(3):118–23.
5. Lestari A, Susanti L, Dwiatmaka Y . Optimasi pelarut o Jenderal Pendidikan Tinggi, yang telah memberikan bantuan dana pendidikan dan penelitian disertasi studi etanol-air dalam proses ekstraksi herba pegagan (Centella asiatica [L.] Urban) pada suhu terukur. Bionatura. 2012. 14:87–93
6. Departemen Kesehatan Republik Indonesia. Farmakope herbal Indonesia. Jakarta: Departemen Kesehatan Republik Indonesia; 2008. 109-15.
7. Molyneux P. The use of stable free radical diphenylpicryl hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 2004. 26(2):211-9.
8. Sh a r m a GN, Du b e y SK, Sa t i N, Sa n a d y a J. Phytochemical screening and estimation of total phenolic content in Aegle marmelos Seeds. International Journal of Pharmaceutical and Clinical Research. 2011. 3(2):27-9.
9. Bajcan D, Harangozo L, Hrabovska D, Boncikova D. Optimizing condition for spectrophotometric determination of total polyphenols in wines using Folin-Ciocalteu reagent. Journal of Microbiology, Biotechnology and Food Sciences. 2013. 2(Special issue 1):1699-708.
Published
2015-04-30
How to Cite
LESTARI, AGATHA BUDI SUSIANA et al. The Effect of Simplex Powder n-Hexane Purification on Asiaticoside Content, Free Radical Scavenging and Total Phenolic Content of Gotu Kola (Centella asiatica (L.) Urban) Ethanolic Extract. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 13, n. 1, p. 10-16, apr. 2015. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/112>. Date accessed: 23 nov. 2024.
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Vol 13 No 1 (2015): JIFI
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