Cytotoxic Potential of Ethanol Extract Akar Kedayan (Aristolochia foveolata Merr.) on Fertilized Tripneustes gratilla Eggs

  • Syuhada Syuhada Pharmacy Study Program, Politeknik Kaltara, Tarakan, North Kalimantan, 77113, Indonesia
  • Benazir Evita Rukaya Pharmacy Study Program, Politeknik Kaltara, Tarakan, North Kalimantan, 77113, Indonesia

Abstract

The cytotoxicity test is a screening test used to evaluate the reaction of live cells to implants in cell culture tests. This study aims to determine the cytotoxic activity of akar kedayan in fertilized Tripneustes gratilla eggs, which have an impact on inhibiting the development of fertile cells. This study used an experimental method by comparing the inhibition of Tripneustes gratilla egg cell division in the ethanol extract group of akar kedayan at concentrations of 10, 100, and 1000 ppm, with negative control, then observing embryo cell division at 1, 2, 4, and 12 hours. After fertilization, a comparative statistical analysis was conducted using the ANOVA test, followed by the Tukey HSD Post Hoc Test. The results showed that the negative control and 10 ppm concentration did not inhibit the division of the fertile Tripneustes gratilla eggs (P-value>0.05), while the ethanol extracts of 100 and 1000 ppm significantly inhibited the division of Tripneustes gratilla eggs (P-value< 0.05). This study concludes that the ethanol extract of akar kedayan has cytotoxic ability by inhibiting the development of Tripneustes gratilla fertile eggs with the optimum cytotoxic dose range at concentrations of 10-100 ppm.

References

1. Ernst E. Aristolochia, a herbal treatment to die for? Maturitas. 2012 Oct 1;73(2):85–6.

2. Grollman AP, Marcus DM. Global hazards of herbal remedies: lessons from Aristolochia. EMBO Rep. 2016;17(5):619–25.

3. Han J, Xian Z, Zhang Y, Liu J, Liang A. Systematic overview of aristolochic acids: Nephrotoxicity, carcinogenicity, and underlying mechanisms. Front Pharmacol. 2019;10(JUN).

4. Herman. Pemanfaatan Akar Kedayan (Aristolochia sp.) Sebagai Obat Tradisional Oleh Masyarakat Suku Dayak Merap Desa Gong Solok Kecamatan Malinau Selatan Kabupaten Malinau [Thesis]. [Samarinda]: Politeknik Pertanian Negeri Samarinda; 2011.

5. Parlupi B, Yudha CR, Padan Y, Andreas, Paksual, Sihin. Tumbuhan hutan sekolah berkhasiat obat SDN 002 Malinau Selatan Hilir. Andriani RR, Tjandrawati OS, editors. Malinau: WWF Indonesia; 2019.

6. Supriningrum R, Warnida H, Sapri. Uji Daya Analgetik Ekstrak Etanol Akar Tabar Kedayan (Aristolochia foveolata Merr.) Pada Mencit Putih Jantan Dengan. Media Sains. 2016;9(April):22–6.

7. Kuo PC, Li YC, Wu TS. Chemical Constituents and Pharmacology of the Aristolochia species. J Tradit Complement Med. 2012 Oct 1;2(4):249–66.

8. Lerma-Herrera MA, Beiza-Granados L, Ochoa-Zarzosa A, López-Meza JE, Hernández-Hernández JD, Aviña-
Verduzco J, et al. In vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines. Saudi J Biol Sci [Internet]. 2021 Aug 9 [cited 2021 Sep 2]; Available from: https://www.sciencedirect.com/science/article/pii/S1319562X2100680X

9. Hosoya N, Motomura K, Tagawa E, Nagano M, Ogiwara C, Hosoya H. Effects of the fungicide ortho-phenylphenol (OPP) on the early development of sea urchin eggs. Mar Environ Res. 2019 Jan 1;143:24–9.

10. Nishioka D, Marcell V, Cunningham M, Khan M, Von Hoff DD, Izbicka E. The Use of Early Sea Urchin Embryos in Anticancer Drug Testing. In: Buolamwini JK, Adjei AA, editors. Novel Anticancer Drug Protocols [Internet]. Totowa, NJ: Humana Press; 2003 [cited 2023 Mar 7]. p. 265–76. (Methods in Molecular Medicine; vol. 85). Available from: https://doi.org/10.1385/1-59259-380-1:265

11. Wagner A, Nacci D. Tropical Collector Urchin, Tripneustes gratilla, Fertilization Test Method. U S Enviromental Prot Agency. 2012;(April).

12. Vanherweghem JL, Tielemans C, Abramowicz D, Depierreux M, Vanhaelen-Fastre R, Vanhaelen M, et al. Rapidly progressive interstitial renal fibrosis in young women: association with slimming regimen including Chinese herbs. The Lancet. 1993 Feb 13;341(8842):387–91.

13. IARC Monographs. volume 100 A: A review of human carcinogens [Internet]. Vol. 100. Lyon: International Agency for Research on Cancer; 2012. Available from: https://monographs.iarc.fr/wp-content/uploads/2018/06/mono100A.pdf

14. Wang L, Ding X, Li C, Zhao Y, Yu C, Yi Y, et al. Oral administration of Aristolochia manshuriensis Kom in rats induces tumors in multiple organs. J Ethnopharmacol. 2018 Oct 28;225:81–9.

15. Aigbe FR, Sofidiya OM, James AB, Sowemimo AA, Akindere OK, Aliu MO, et al. Evaluation of the toxicity
potential of acute and sub-acute exposure to the aqueous root extract of Aristolochia ringens Vahl. (Aristolochiaceae). J Ethnopharmacol. 2019 Nov 15;244:112150.

16. Sborchia M, Keun HC, Phillips DH, Arlt VM. The impact of p53 on aristolochic acid i-induced gene expression in vivo. Int J Mol Sci. 2019;20(24):1–25.

17. Jubaidah SJ, Nurhasnawati H. Analisis Flavonoid Total Akar Tabar Kedayan (Aristolochia foveolata Merr). Al-Kim. 2018 Dec 24;6(2):135–40.

18. Mabrur Achmad. Determinasi Tanaman Akar Kedayan. UPT Labiratorium Herbal Materia Medica Batu; 2021.

19. United States Environmental Protection Agnecy. Sperm Cell Toxicity Tests Using the Sea Urchin. 2009.

20. Vacquier VD. Laboratory on sea urchin fertilization. Mol Reprod Dev. 2011 Aug;78(8):553–64.

21. Ghorani V, Mortazavi MS, Mohammadi E, Sadripour E, Soltani M, Shahri NM, et al. Determination of developmental stages of embryo in the Sea Urchin, Echinometra mathaei. 2012;11(2):294–304.

22. Andreani T, Severino P, de Hollanda LM, Vazzana M, Souto SB, Santini A, et al. Chapter 9 - Cancer therapies: applications, nanomedicines, and nanotoxicology. In: Ficai A, Grumezescu AM, editors. Nanostructures for Cancer Therapy [Internet]. Elsevier; 2017 [cited 2021 Sep 30]. p. 241–60. (Micro and Nano Technologies). Available from: https://www.sciencedirect.com/science/article/pii/B978032346144300009X

23. Cai Y, Cai TG. Two New Aristolochic Acid Derivatives from the Roots of Aristolochia fangchi and Their Cytotoxicities. Chem Pharm Bull (Tokyo). 2010;58(8):1093–5.

24. Chung YM, Chang FR, Tseng TF, Hwang TL, Chen LC, Wu SF, et al. A novel alkaloid, aristopyridinone A and anti-inflammatory phenanthrenes isolated from Aristolochia manshuriensis. Bioorg Med Chem Lett. 2011 Mar 15;21(6):1792–4.

25. Battu GR, Parimi R, Shekar KBC. In vivo and in vitro pharmacological activity of Aristolochia tagala (syn: Aristolochia acuminata) root extracts. Pharm Biol. 2011 Nov 1;49(11):1210–4.
Published
2023-04-29
How to Cite
SYUHADA, Syuhada; RUKAYA, Benazir Evita. Cytotoxic Potential of Ethanol Extract Akar Kedayan (Aristolochia foveolata Merr.) on Fertilized Tripneustes gratilla Eggs. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 21, n. 1, p. 65-70, apr. 2023. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1130>. Date accessed: 17 apr. 2024. doi: https://doi.org/10.35814/jifi.v21i1.1130.
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Articles