Antioxidant Activity Assay from N-Hexane Fraction of Binjai Mangifera caesia Jack. Ex. Wall. Leaves Methanolic Extract Using DPPH Method

English

  • Hafiz Ramadhan STIKES Borneo Lestari
  • Siti Purnama STIKES Borneo Lestari
  • Putri Indah Sayakti STIKES Borneo Lestari
DOI: https://doi.org/10.35814/jifi.v20i1.1133

Abstract

Natural antioxidant compounds can be found in plants of the Mangifera genus, one of them is Mangifera caesia, Jack. ex. Wall or which was also known as binjai. This study aims to determine group compounds and the antioxidant activity of the n-hexane fraction from the binjai leaves methanol extract against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. The binjai leaves powder extraction was carried out using the soxhlet apparatus with methanol and liquid-liquid fractionation using water and n-hexane. Phytochemical screening was done by adding specific reagents. The antioxidant activity assay qualitatively on TLC that being sprayed with DPPH and quantitative assay by measuring the absorbance of the fraction attenuation against DPPH radicals using a UV-Vis Spectrophotometer. The Phytochemicals screening results showed that the n-hexane fraction contained phenolic, flavonoid, and steroids. The qualitative test results showed some compounds had an antioxidant activity that appeared by changing the color of the spots to yellow on a purple background after spraying with DPPH solution. The n-hexane fraction resulted in an IC50 value of 34.0668 ppm with quercetin IC50 of 2.4469 ppm. Based on these results, it can be concluded that the n-hexane fraction of binjai leaves methanol extract contains phenolic and flavonoid compounds that played a role in producing very strong antioxidant activity.

References

1. Moussa Z, Judeh ZMA, Ahmed SA. Nonenzymatic Exogenous, and Endogenous Antioxidants. In: Das K, Editor. Free Radical Medicine and Biology. London: IntechOpen; 2019, p. 1-22.

2. Ramadhan H, Baidah D, Lestari NP, Yuliana KA. Aktivitas antioksidan ekstrak etanol 96% daun, buah dan kulit Terap (Artocarpus odorratissimus) menggunakan metode CUPRAC. Farmasains. 2020.7(1):7-12.

3. Khairiah K, Taufiqurrahman I, Putri DKT. Antioxidant Activity Test Of Ethyl Acetate Fraction Of Binjai (Mangifera caesia) Leaf Ethanol Extract. Dental Journal (Majalah Kedokteran Gigi). 2018.51(4):164-68.

4. Ismail NA, Abu Bakar MF, Abu Bakar FI, Rahim AC, Murdin N. Underutilized Mangifera species (Mangifera caesia, Mangifera quadrifida, and Mangifera odorata) from Borneo: A potential source of antioxidant. J. Eng. Appl. Sci. 2019.14(4):1169-77.

5. Ramadhan H, Hipmi AF, Sayakti PI. Antioxidant activity of Binjai leaves (Mangifera caesia Jack. Ex. Wall) methanol extract from South Kalimantan using DPPH and CUPRAC methods. Proceeding Book The 1st ITB International Conference on Pharmaceutical Sciences and Pharmacy. Bandung, 2020:9–19.

6. Norliyanti, Taufiqurrahman I, Sukmana BI. Comparison of Antioxidant Activity between Socletation and Maceration Extraction Method on Binjai Leaf Extract. Jurnal Kedokteran Gigi. 2018.3(2):182-88.

7. Ramadhan H, Sayakti PI, Ulya R, Hidayati M, Putri ZP, Rauf A, Nafila. Fenol-flavonoid dan aktivitas antioksidan fraksi air dan etil asetat dari daun Binjai (Mangifera caesia Jack. Ex. Wall). Jurnal Ilmiah Pharmacy. 2022.9(1):49-58.

8. Adham D, Taufiqurrahman I, Helmi ZN. Flavonoid Level Analysis of Binjai Leaf Extract (Mangifera caesia) in Ethanol, Methanol, and N-Hexane Solvents. Dentino Jurnal Kedokteran Gigi. 2019.4(1):46-49.

9. Putri AD, Taufiqurrahman I, Dewi N. Antioxidant Activity of Binjai Leaves (Mangifera caesia) Ethanol Extracts. Dentino Jurnal Kedokteran Gigi. 2019.4(1):55–5\9.

10. Rosita JM, Taufiqurrahman I, Edyson. Perbedaan Total Flavonoid Antara Metode Maserasi dengan Sokletasi pada Ekstrak Daun Binjai (Mangifera caesia). Dentino Jurnal Kedokteran Gigi. 2017.1(1):100-5.

11. Dwidhanti F, Taufiqurrahman I, Sukmana BI. Cytotoxicity Test of Binjai Leaf (Mangifera caesia) Ethanol Extract in Relation to Vero Cells. Dental Journal. 2018.51(3):108-13.

12. Shinde SS & Chavan AR. Isolation of Mangiferin from Different Varieties of Mangifera indica Dried Leaves. International Journal of Scientific & Engineering Research. 2014.5(6):928-34.

13. Ramadhan H, Rezky DP, Susiani EF. Penetapan Kandungan Total Fenolik-Flavonoid pada Fraksi Etil Asetat Kulit Batang Kasturi (Mangifera casturi Kosterman). Jurnal Farmasi dan Ilmu Kefarmasian Indonesia. 2021.8(1):58-67.

14. Fitriyanti, Norhavid MFR, Ramadhan H. Uji Daya Hambat Ekstrak Etanol 70% Biji Pepaya (Carica papaya L.) Terhadap Bakteri Propionibacterium acnes Penyebab Jerawat. Pharmacoscript. 2020.3(2):144-9.

15. Ramadhan H, Arsyad M, Sayakti PI. Skrining Fitokimia Dan Uji Aktivitas Antibakteri Ekstrak Etanol 70% Biji Kalangkala (Litsea angulata Bl.) Terhadap Bakteri Penyebab Jerawat Propionibacterium acnes. Borneo Journal of Pharmascientech. 2020.4(1):60-70.

16. Ramadhan H, Andina L, Vebruati, Nafila, Yuliana KA, Baidah D, Lestari NP. Perbandingan Rendemen dan Skrining Fitokimia dari Ekstrak Etanol 96% Daun, Buah dan Kulit Buah Terap (Artocarpus odoratissimus Blanco). Jurnal Ilmiah Farmako Bahari. 2020.11(2):103-12.

17. Sarker SD and Nahar L. Natural Products Isolation. In: Walker JM, editor. Methods in Molecular Biology. Totowa, NJ: Humana Press; 2012. p. 20,339-42.

18. Mirfat AHS, Salma I, Razali M. Natural antioxidant properties of selected wild Mangifera species in Malaysia. J. Trop. Agric. Food Sci. 2016.44(1):63-72.

19. Dwiatun, I. Uji Aktivitas Antioksidan Fraksi N-Heksan, Etil Asetat, Dan Fraksi Air Ekstrak Metanol Daun Mangga Kasturi (Mangifera casturi Kosterm.) Terhadap DPPH. Skripsi. Fakultas Farmasi Universitas Setia Budi, Surakarta. 2018.

20. Eden WT, Buanasari, Setyowati. Aktivitas Antioksidan Frasksi dan Ekstrak Metanol Daun Mangga (Mangifera indica L.) dengan Metode Serapan DPPH Menggunakan Spektrofotometer UV-Vis. Prosiding SNKPK. 2016:305-15.

21. Pamungkas DK, Retnaningtyas Y, Wulandari L. Pengujian Aktivitas Antioksidan Kombinasi Ekstrak Metanol Daun Mangga Gadung (Mangifera indica L. var. gadung) dan Ekstrak Etanol Daun Pandan Wangi (Pandanus amaryllifolius Roxb.). e-Jurnal Pustaka Kesehatan. 2017. 5(1):47-9.

22. Wijaya H, Novitasari, Jubaidah S. Perbandingan Metode Ekstraksi Terhadap Rendemen Ekstrak Daun Rambai Laut (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung. 2018.4(1):79-83.

23. Saidi N, Ginting B, Murniana, Mustanir. Analisis Metabolit Sekunder. Banda Aceh: Syiah Kuala University Press; 2018. p. 12-29.

24. Sayuti K and Yenrina R. Antioksidan Alami dan Sintetik. Padang: Andalas University Press; 2015.

25. Kumar S and Pandey AK. Chemistry and Biological Activities of Flavonoids: An Overview. The Scientific World Journal. 2013:1–16.

26. Putri AAS and Hidayati N. Uji Aktivitas Antioksidan Senyawa Fenolik Ekstrak Metanol Kulit Batang Tumbuhan Nyiri Batu (Xylocarpus moluccensis). UNESA Journal of Chemistry. 2015.4(1):1-6.

27. Yuliani CR, Ramadhan H, Sayakti PI, Torizellia C. Total Phenolic and Flavonoid Contents of n-Hexane Fraction in Binjai Leaves (Mangifera caesia Jack. ex. Wall). Jurnal Ilmiah Farmasi. 2022. Special Edition:11-2.

28. Paulinus YVG, Jayuska A, Ardiningsih P, Nofiani R. Aktivitas Antioksidan dan Kandungan Total Fenol Fraksi Etil Asetat Buah Palasu (Mangifera caesia Jack). Jurnal Kimia Khatulistiwa. 2015.4(1):47-50.

29. Sulaiman SF and Ooi KL. Polyphenolic and Vitamin C Contents and Antioxidant Activities of Aqueous Extracts from Mature-Green and Ripe Fruit Fleshes of Mangifera sp. J Agric Food Chem. 2012. 60(47):11832-8.

30. Anwar K, Fadillaturahmah, Sari DP. Analisis Kandungan Flavanoid Total Ekstrak Etanol Daun Binjai (Mangifera caesia Jack.). Jurnal Ilmiah Ibnu Sina. 2017.2(1):20-30.

31. Ahmad S, Sukari MA, Ismail N, Ismail IS, Abdul AB, Abu Bakar MF, et al. Phytochemicals from Mangifera pajang Kosterm and Their Biological Activities. BMC Complement Altern Med. 2015. 15(1):1-8.

32. Murwanto PE and Santosa D. Uji Aktivitas Antioksidan Tumbuhan Cynara scolimus L., Artemisia china L., Borreria repens DC., Polygala paniculata L. Hasil Koleksi dari Taman Nasional Gunung Merapi Dengan Metode Penangkapan Radikal DPPH (2,2-Difenil-1-Pikrilhidrazil). Majalah Obat Tradisional. 2012.17(3):53-60.

33. Karim K, Jura MR, Sabang SM. Uji Aktivitas Antioksidan Ekstrak Daun Patikan Kebo (Euphorbia hirta L.). Jurnal Akademika Kimia. 2015. 4(2):57-63.
Published
2022-04-29
How to Cite
RAMADHAN, Hafiz; PURNAMA, Siti; SAYAKTI, Putri Indah. Antioxidant Activity Assay from N-Hexane Fraction of Binjai Mangifera caesia Jack. Ex. Wall. Leaves Methanolic Extract Using DPPH Method. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 20, n. 1, p. 55-62, apr. 2022. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1133>. Date accessed: 22 dec. 2024. doi: https://doi.org/10.35814/jifi.v20i1.1133.
Citation Formats
Issue
Vol 20 No 1 (2022): JIFI
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.