Antimalarial Activity of Multiple Dose and Single Dose Administration of Artocarpus Champeden Spreng. Stembark Extract on Plasmodium Berghei Infected Mice



 Artocarpus champeden Spreng. (Moraceae) known as “cempedak”, was traditionally used for antimalarial remedies in Indonesia. Study to determine antimalarial activity of ethanol extract of cempedak stembark was carried out. Artocarpus champeden Ethanol Extract (ACEE) was administered twice per day (multiple dose) and once per day (single dose) orally. The study was carried out by modification method of the “4 Days Suppressive Test” originally described by Peter. The result showed that ACEE administered twice per day inhibited parasites growth (ED 0.19 mg/kg BW) higher than once per day (ED 6.0 mg/kg BW) and 10 mg/kg body weight of dose administrated twice per day was more effective than 100 mg/kg body weight once per day. 


1. WHO. World Malaria Report: 2012. WHO Press. Geneva. 2012. [1 tayangan], diambil dari http:// report_2012/wmr2012_full_report.pdf. diakses Juni 2014.
2. Bloland PB. Drug resistance in malaria. Malaria Epidemiology Branch Centers for Disease Control and Prevention (WHO/CDS/CSR/DRS/2001.4). Chamblee GA. United States of America. 2001. [1 tayangan]. diambil dari: publications/drugresist/malaria.pdf. diakses Juni 2014.
3. Has tings IM.The origins of antimalarial drug resistance. Trends Parasitol. 2004. Nov 20(11):512-8.
4. Kim Y , Schneider KA. Evolution of drug resistance in malaria parasite population.
5. World Health Organzation. WHO traditional medicine strategy:2014-2023. WHO Press. Geneva. 2014. [1 tayangan], diambil dari bitstream/10665/92455/1/9789241506090_eng.pdf. diakses Juni 2014.
6. Hakim EH, Achmad SA, Juliawaty LD, Makmur L, Syah YM, Aimi N, et al. Prenylated flavonoids and related compounds of the Indonesian Artocarpus (Moraceae). J Nat Med. 2006. 60:161-84.
7. Achmad SA, Hakim EH, Juliawaty LD, Makmur L, Suyatno. A new prenylated flavone from Artocarpus champeden. J Nat Prod. 1996. 9:878-9.
8. Widyawaruyanti A, Subehan, Kalauni SK, Awale S, Nindatu M, Zaini NC, et al. New prenylated flavones from Artocarpus champeden and their antimalarial activity in vitro. J Nat Med. 2007.61:410-3.
9. Utomo DNW. Aktivitas antimalaria ekstrak methanol kulit batang cempedak (Artocarpus champeden Spreng.) terhadap Plasmodium berghei in-vivo [skripsi]. Surabaya: Fakultas Farmasi Unair; 2003.
10. Hidayati AR. Uji aktivitas antimalaria fraksi kloroform kulit batang cempedak (Artocarpus champeden Spreng.) terhadap Plasmodium berghei in-vivo. [skripsi]. Surabaya: Fakultas Farmasi Unair; 2004.
11. Widyawaruyanti A, Hafid AF, Ekasari W, Sjafruddin, Zaini NC. Ekstrak terstandar kulit batang cempedak (Artocarpus champeden Spreng.) sebagai bahan baku obat fitofarmaka antimalaria potensial, Laporan Penelitian. Surabaya: Fakultas Farmasi Universitas Airlangga; 2007.
12. Hafid AF, Tyas MW, Widyawaruyanti A. Model terapi kombinasi ekstrak etanol 80% kulit batang cempedak (Artocarpus champeden Spreng.) dan artesunat pada mencit terinfeksi malaria. J Indon Med Assoc. 4 April 2011. 61(4).
13. Hafid AF, Ariantari NP, Tumewu L, Hidayati AR, Widyawaruyanti A. The active marker compound identification of Artocarpus champeden Spreng. stembark extract, morachacone A as antimalarial. Int J Pharm Pharm Sci. 2012. 4(S5).246-9.
14. Shar gel L, W u-Pong S, Yu AB. Applied biopharmaceutics and pharmacokinetics. 5th Ed. Boston: Mc Graw Hill; 2005.
15. Philipson JD. Assays for antimalarial and amoebicidal activities. In: Day PM, Harborne JB, editors. Methods in plant biochemistry. Vol 6. London: Academic Press; 1991. 135-52.
16. Fidock DA, Rosenthal PJ, Croft SL, Brun R, Nwaka S. Antimalarial drug discovery: Efficacy models for compound screening review. Nature. 2004. 509-20.
How to Cite
SARI, SOFIANA; HAFID, ACHMAD FUAD; WIDYAWARUYANTI, ATY. Antimalarial Activity of Multiple Dose and Single Dose Administration of Artocarpus Champeden Spreng. Stembark Extract on Plasmodium Berghei Infected Mice. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 13, n. 1, p. 23-28, apr. 2015. ISSN 2614-6495. Available at: <>. Date accessed: 31 may 2023.