Antioxidant Effect of Cosolvent Solution of Mangosteen (Garcinia mangostana L.) Rind Extract in Rats by Using MDA and SOD Parameter

  • ROS SUMARNY UNIVERSITAS PANCASILA
  • LILIEK NURHIDAYATI UNIVERSITAS PANCASILA
  • SITI SOFIAH UNIVERSITAS PANCASILA
  • YATI SUMIYATI UNIVERSITAS PANCASILA
  • FRANSISKA DIANA SANTI UNIVERSITAS PANCASILA

Abstract

 Mangosteen (Garcinia mangostana L.) rinds extract in capsule form has been marketed. a-mangostin, the active compound of G. mangostana had been known has low solubility in water. This study was aimed to measure and compare antioxidant effect of a-mangostin as cosolvent solution to extracts suspentions. 25 rats were divided into 5 groups: negative (I) and positive (II) control, cosolvent solution (III), extract (IV) and extract® (V). Treatment were given for 14 days, free radicals were induced by CCl . MDA were analyzed using thiobarbituric acid method while SOD by using adenochrome assay. Results showed MDA level were: 1.0±0.19; 0.3±0.05; 0.4±0.04; 0.6±0.04 and 0.5±0.00 nmol/mL, whereas SOD activity were: 17±0.0; 123±25.3; 107±19.0; 73±18.9 and 97±27.0 U/mL for group I, II, III, IV and V, respectively. Kruskal Wallis test showed a significant difference in MDA between group III with IV and V (p>0.05), showing cosolvent has higher ability to decrease MDA than the extracts suspension. There were significant differences of SOD between group III and IV (p>0.05), meaning cosolvent has higher ability to increase SOD activity compare to the extracts. Research showed cosolvent has a higher antioxidant effect with potential to inhibit MDA formation by 70% and increase SOD activity by 623%. 

References

1. Aisha AFA, Abu-Salah KM, Ismail Z, Majid AMSA. Determination of total xanthones in Garcinia mangostana fruit rind exstracts by ultraviolet (UV) spectrophotometry. J Med Plants Res. 2013.7(1):29.
2. Jung AH, Su BN, Keller, Mehta RG, Kinghorn AD. Antioxidant xanthones from the pericarps of Garcinia mangostana (Mangosteen). J Agric Food Chem. 2006.54:2077-82.
3. Fatimah. Formulasi larutan oral ekstrak kulit buah manggis (Garcinia mangostana L.) sebagai antioksidan dengan teknik kosolvensi [skripsi]. Jakarta: Fakultas Farmasi Universitas Pancasila; 2013. 24-32.
4. Caesar K. Optimasi dan validasi metode penetapan kadar α-mangostin dalam larutan oral ekstrak kulit buah manggis (Garcinia mangostana L.) secara KCKT [skripsi]. Jakarta: Fakultas Farmasi Universitas Pancasila; 2013. 42.
5. Snell K, Mullocl B. Biochemical toxicology. Ed 5. Washington DC: IRL press Oxford; 1987.138.
6. Misra and Fridovich. The role of superoxide dismutase anion in auto-oxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972. 247(10):3170-5.
7. Panjaitan RGP, Handharyani E, Chairul, Masriani, Zakiah Z. Pengaruh pemberian karbon tetraklorida terhadap fungsi hati dan ginjal tikus. Makara Kesehatan. 2007.11(1):11-6.
8. Muhammad I. Efek antioksidan vitamin C terhadap tikus (Rattus norvegicus L) jantan akibat pemaparan asap rokok [tesis]. Bogor: Sekolah Pasca Sarjana Institut Pertanian Bogor; 2009. 3,19-20.
9. Arsana IN, Adiputra, N, Pangkahila, JA, Putra- Manuaba IB. Garcinia mangostana L. rind extract and physical training reduce oxidative stress in wistar rats during maximal physical activity. J Bio Sci. 2013. 7(2):63-8.
10. Winarsi H. Antioksidan alami & radikal bebas: Potensi dan aplikasinya dalam kesehatan. Yogyakarta: Kanisius; 2007.15, 11-22, 56-7, 77-81, 86-96, 141.
11. Bub A, Bernhard W, Abrahamse L, et al. Moderate intervention with carotenoid-rich vegetable products reduces lipid peroxidation in men. J Nutr. 2000. 130:2200-6
How to Cite
SUMARNY, ROS et al. Antioxidant Effect of Cosolvent Solution of Mangosteen (Garcinia mangostana L.) Rind Extract in Rats by Using MDA and SOD Parameter. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 13, n. 1, p. 35-39, apr. 2015. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/117>. Date accessed: 23 nov. 2024.
Section
Articles