Antibacterial Effect Combination of Moringa Leaf Extract (Moringa oleifera Lam.) and Amoxicillin against Staphylococcus aureus and Escherichia coli in Vitro

  • Setiadi Abdillah Pratama Faculty of Medicine, Universitas Sumatera Utara
  • Tri Widyawati Faculty of Medicine, Universitas Sumatera Utara
  • Muhammad Ichwan Faculty of Medicine, Universitas Sumatera Utara
  • Dian Dwi Wahyuni Faculty of Medicine, Universitas Sumatera Utara


Staphylococcus aureus and Escherichia coli often infect humans and risky cause antibiotics resistance including amoxicillin. This encourages us finding alternative active ingredients: Moringa oleifera leaf. This study evaluated antibacterial activity of combination of Moringa leaf extract (KDK) and amoxicillin (KA) against S. aureus and E. coli in vitro. The research methods with true experimental research with post-test control group design. KDK obtained by maceration. Four concentrations of KDK test materials (20, 30, 40, 50%) and 1 concentration of KA (3 mg/mL). Disc diffusion test method taken and the parameter measured: the diameter of the disc inhibition zone. Data analyzed by One-way ANOVA and further Tukey’s test. KDK+KA against S. aureus (20.77±1.79 – 21.33±1.74 mm)>KDK (11.45±0.21 – 12.45±0.28 mm) or KA (14.50±0.42 mm). KDK+KA against E. coli (15.53±0.71 – 17.87±0.42 mm)>KDK (9.00±0.28 – 10.30±0.42 mm) or KA (13.45±0.35 mm). A significant (p<0.05) was obtained between the combination group (KDK+KA) versus a group (KDK or KA). The combination of Moringa leaf ethanol extract and amoxicillin synergistically effected as antibacterial against S. aureus and E. coli in vitro.


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How to Cite
PRATAMA, Setiadi Abdillah et al. Antibacterial Effect Combination of Moringa Leaf Extract (Moringa oleifera Lam.) and Amoxicillin against Staphylococcus aureus and Escherichia coli in Vitro. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 20, n. 1, p. 23-29, apr. 2022. ISSN 2614-6495. Available at: <>. Date accessed: 24 june 2024. doi: