Antibacterial Effect Combination of Moringa Leaf Extract (Moringa oleifera Lam.) and Amoxicillin against Staphylococcus aureus and Escherichia coli in Vitro

  • Setiadi Abdillah Pratama Faculty of Medicine, Universitas Sumatera Utara
  • Tri Widyawati Faculty of Medicine, Universitas Sumatera Utara
  • Muhammad Ichwan Faculty of Medicine, Universitas Sumatera Utara
  • Dian Dwi Wahyuni Faculty of Medicine, Universitas Sumatera Utara
DOI: https://doi.org/10.35814/jifi.v20i1.1174

Abstract

Staphylococcus aureus and Escherichia coli often infect humans and risky cause antibiotics resistance including amoxicillin. This encourages us finding alternative active ingredients: Moringa oleifera leaf. This study evaluated antibacterial activity of combination of Moringa leaf extract (KDK) and amoxicillin (KA) against S. aureus and E. coli in vitro. The research methods with true experimental research with post-test control group design. KDK obtained by maceration. Four concentrations of KDK test materials (20, 30, 40, 50%) and 1 concentration of KA (3 mg/mL). Disc diffusion test method taken and the parameter measured: the diameter of the disc inhibition zone. Data analyzed by One-way ANOVA and further Tukey’s test. KDK+KA against S. aureus (20.77±1.79 – 21.33±1.74 mm)>KDK (11.45±0.21 – 12.45±0.28 mm) or KA (14.50±0.42 mm). KDK+KA against E. coli (15.53±0.71 – 17.87±0.42 mm)>KDK (9.00±0.28 – 10.30±0.42 mm) or KA (13.45±0.35 mm). A significant (p<0.05) was obtained between the combination group (KDK+KA) versus a group (KDK or KA). The combination of Moringa leaf ethanol extract and amoxicillin synergistically effected as antibacterial against S. aureus and E. coli in vitro.

References

1. Laupland, et al. Burden of bloodstream infection in older persons: a population‐based study. BMC Geriatrics. 2021; 21:31. https://doi.org/10.1186/s12877-020-01984-z. Accessed September 11, 2021.

2. Kern, W.V and Rieg, S. Burden of bacterial bloodstream infection-a brief update on epidemiology and significance of multidrug-resistant pathogens. Clinical microbiology and infection 26. 2020; 151-157. https://doi.org/10.1016/j.cmi.2019.10.031. Accessed September 21, 2021.

3. Karlowsky, et al. Trends in antimicrobial resistance among urinary tract infection isolates of Escherichia coli from female outpatients in the United States. Antimicrobial agents and chemotherapy. 2002; 2540–2545. https://doi.org/10.1128/AAC.46.8.2540–2545.2002. Accessed August 21, 2021.

4. Islam, M, et al. Antibacterial activity of crab of amoxicillin against Staphylococcus aureus and Eschericia coli. Journal of Advanced Scientific Research. 2011; 2(4): 63-66.

5. Abou-Zeid, S.M et al. Moringa oleifera ethanolic extract attenuates tilmicosin-induced renal damage in male rats via suppression of oxidative stress, inflammatory injury, and intermediate filament proteins mRNA expression. Biomedicine and pharmacotherapy. 2021; 133.

6. Gomes, F et al. Plant phenolic extracts as an effective strategy to control Staphylococcus aureus, the dairy industry pathogen. Ind. Crops Prod. 2018; 112; 515–520.

7. Elza, S., Fakhrurrazi., Harris A. Uji antibakteri ekstrak daun kelor (Moringa oleifera l.) terhadap pertumbuhan bakteri Staphylococcus aureus. JIMVET. 2018; 2(3):373-379.

8. Chanda, S., and Rakholiya, K. Combination therapy: synergism between natural plant Extracts and Antibiotics Against Infectious Diseases. 2011; 520-529.

9. Kementerian Kesehatan Republik Indonesia. Daun kelor (Moringa oleifera folium). Farmakope herbal Indonesia 2. 2017; 209.

10. Dwidjoseputro. Dasar-Dasar Mikrobiologi. Penerbit Djambatan. 1998.

11. Maida, S. and Lestari, K. Aktivitas antibakteri amoksisilin terhadap bakteri gram positif dan bakteri gram negatif amoxiciillin. Jurnal Pijar MIPA. 2019; 8(5): 55. https://doi.org/10.29303/jpm.1029. Accessed August 21, 2021.

12. Agustie, A and Samsumaharto, R. Antibacterial activity test of moringa leaf extract (Moringa oleifera lam.) against Staphylococcus aureus bacteria. Biomedika 6 (2). 2013; 14-19.

13. Amalia, M. Uji aktivitas antibakteri ekstrak metanol daun kelor (Moringa oleifera l.) terhadap bakteri Escherichia coli dengan metode sumuran. 2021; 1-4.

14. Emelia, R, Safitri D, and Andriyani, H. Daun kelor (Moringa oleifera) sebagai antibakteri terhadap infeksi bakteri Escherichia coli. Jurnal infokes-politeknik piksi ganesha. 2021; 44-50.

15. Purnasari, C. Uji aktivitas antibakteri ekstrak etanol daun kelor (Moringa oleifera Lam.) terhadap bakteri patogen resisten antibiotik. 2013; 21-29.

16. Yunita, E, Permatasari, D, and Lestari D. Antibacterial activity of moringa leaves extract against Pseudomonas aeruginosa. Jurnal Ilmiah Farmako Bahari. 2020; 189-194. https://journal.uniga.ac.id/index.php/JFB. Accessed August 21, 2021.
Published
2022-04-25
How to Cite
PRATAMA, Setiadi Abdillah et al. Antibacterial Effect Combination of Moringa Leaf Extract (Moringa oleifera Lam.) and Amoxicillin against Staphylococcus aureus and Escherichia coli in Vitro. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 20, n. 1, p. 23-29, apr. 2022. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1174>. Date accessed: 05 nov. 2024. doi: https://doi.org/10.35814/jifi.v20i1.1174.
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Vol 20 No 1 (2022): JIFI
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