Safety Evaluation of Curcumin Nanoparticle Formula in Mice and Antioxidants Potency In-Vitro

  • Ni Made Dwi Sandhiutami Fakultas Farmasi Universitas Pancasila
  • Rika Sari Dewi Fakultas Farmasi, Universitas Pancasila
  • Sondang Khairani Fakultas Farmasi, Universitas Pancasila
  • Sang Ayu Made Widyadari Fakultas Farmasi, Universitas Pancasila

Abstract

Curcumin has various activities such as antioxidant, anti-inflammatory, antitumor, and antibacterial. The bioavailability of curcumin is low and needs to be optimized. The manufacture of curcumin nanoparticles with several solvents is one way to increase the bioavailability of curcumin. This study aims to examine the safety of curcumin nanoparticles through acute and subchronic toxicity tests and the antioxidant activity of the formula. Acute toxicity test was performed by OECD 425 method, using AOT425StatPgm device for the LD50. Subchronic toxicity test was carried out by OECD 407 method using 4 groups: normal, a dose of 50;100; and 200 mg/kg BW for 28 days, then evaluate body weight, clinical biochemistry, and hematology. An antioxidant activity test was carried out using DPPH. The results showed were the LD50 value of curcumin nanoparticles was>5000 mg/kg BW and the results of the subchronic toxicity test (p>0.05) did not show any significant differences in AST, ALT, BUN, creatinine, albumin, and hematology compare to the normal group. The IC50 of curcumin and curcumin nanoparticles were 118.76 µg/mL and 90.87 µg/mL, respectively. It can be concluded that curcumin nanoparticles are safe and do not affect kidney and liver function and the antioxidant activity of the curcumin nanoparticle formulation is greater than that of curcumin.

References

1. Aggarwal, B. B., dan Sung, B. Pharmacological Basis for the Role of Curcumin in Chronic Diseases: an Age-Old Spice with Modern Targets. Trend in Pharm. Sci. 2009;30:85-94.

2. Bisht S et al. Polymeric nanoparticle - encapsulated curcumin (nanocurcumin): a novel strategy for human cancer therapy. J of Nanobiotechnol. 2007;5:1-18.

3. Pan, M. H., Huang, T. M., dan Lin, J. K. Biotransformation of Curcumin Through Reduction and Glucuronidation in Mice. Drug Metab. Dispos. 1999;27:486-494.

4. Shimatsu A, Kakeya H, Imaizumi A et al. Clinical Application of “Curcumin, a Multi-Functional Substance. Anti Aging Medicine. 2012;9(2):75-83.

5. Arozal, W. L., Rahmat, D., Chendrana, P., and Sandhiutami, N. M. D. Development, characterization and pharmacokinetic profile of chitosansodium tripolyphosphate nanoparticles based drug delivery systems for curcumin. Adv. Pharmaceut. Bull. 2020;11(1):77–85.

6. Donaldson K, Stone V, Tran CL, Kreyling W and Borm PJA. Nanotoxicology. Occup Environ Med. 2004;61:727-8.

7. Akhila JS, Shyamjith, Deepa, Alwar MC. Acute toxicity studies and determination of median lethal dose. Science. 2007;93(7):917-20.

8. Lu, F.C. Basic Toxicology: Fundamentals, Target Organs, and Risk Assesment 5th ed. Informa Healthcare USA, Inc., New York. 2009;85, 108, 188-190.

9. Setzer, R.W. and Kimmel, C.,A. Use of NOAEL, benchmark dose, and other models for human risk assessment of hormonally active substances. Pure Appl Chem. 2003;75:2151–8.

10. Borra SK, Gurumurthy P, Mahendra J. Antioxidant and free radical scavenging activity of Triphala. J. Med. Plant Res. 2013;7:2680–90.

11. Chendrana, Priska. Karakterisasi, Uji Sifat Mukoadhesif dan Aktivitas Anti-Inflamasi Nanopartikel Kurkumin. Jakarta: Fakultas Farmasi Universitas Pancasila; 2019.

12. Organization for Economic Co-operation and Development (OECD). OECD Guidelines for Testing of Chemicals. Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure. (http://lysander.sourceoecd.org/). Paris: OECD. 2001a;1-26.

13. Organization for Economic Co-operation and Development (OECD). OECD Guidelines for Testing of Chemicals. Test No. 407: Acute Oral Toxicity-Repeated Dose 28-Day Oral Toxicity Study in Rodents. Paris: OECD; 2008; 2-5.

14. Grimaldi G, Manto M. Neurological tremor: sensors, signal processing and emerging applications. Sensors 2010;10:1399-422.

15. Posner JB, Saper CB, Schiff ND, Plum F. Plum and Posner's Diagnosis of Stupor and Coma. New York: Oxford University Press;2007.

16. Laksmindra F dkk. Pengaruh Antikoagulan dan waktu Penyimpanan Terhadap Profil Hematologis Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar. 2016;33(1):22-30.

17. Kusumawati, D. Bersahabat Dengan Hewan Coba. Yogyakarta: Gajah Mada University Press; 2004.

18. Sutedjo AY. Mengenal Penyakit Melalui Hasil Pemeriksaan Laboratorium. Yogyakarta: Amara Books. 2009; 28.

19. Maylina, L.A. Hubungan Antara Konsumsi Pangan Sumber Protein, Zat Besi dan Vitamin C dengan Kejadian Anemia Siswa Sekolah Dasar [Skripsi]. Jember: Universitas Jember; 2010.

20. Sadikin, M. Biokimia Darah. Jakarta: Widyamedika; 2008.

21. Gandasoebrata R. Penuntun Laboratorium Klinik. Jakarta: Dian Rakyat; 2008.

22. Wiley, John. Exotic Animal Laboratory Diagnosis. USA: John Wiley; 2020.

23. Kosasih, E.N dan A.S Kosasih. Tafsiran Hasil pemeriksaan Laboratorium Klinik. Edisi Kedua. Tangerang: Karisma Publishing Group; 2008.

24. A.V. Hoffbrand, J.E. Petit, et al. Kapita Selekta Hematologi. Edisi 4. Jakarta: Penerbit Buku Kedokteran EGC; 2009.

25. Mitruka, B. M., H. M. Rawnsley and B. V. Vadehra. Clinical Biochemical and Haematological Reference Value in Normal Experimental Animals. New York: Masson Publishing, Inc; 1977.

26. Lamb, E., Newman, D.J., & Price, C.P. Kidney Function Test.(in) Bur tis,C.A., Ashwood, E.R. & Br uns, D.E.(editors). Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Fourth edition. USA: Elsevier Saunders; 2008. p.797-832.

27. Murray, R. K., Granner, D. K., & Rodwell, V. W. Biokimia harper (27 ed.). Jakarta: Buku Kedokteran EGC; 2009.

28. Kidney Failure. High creatinine level. Jakarta: EGC; 2013.

29. Thapa BR, Walia A. Liver function test and their interpretation. Indian Journal of Pediatrics. 2007; 74:663-671.

30. Guyton A.C. and J.E. Hall. Buku Ajar Fisiologi Kedokteran. Edisi 9. Jakarta: EGC; 2007. p. 74,76, 80-81, 244, 248, 606,636,1070,1340.

31. Hasan I dan Indra T A. Peran Albumin dalam Penatalaksanaan Sirosis Hati. Jakarta: (ID). Divisi Hepatologi Departemen Ilmu Penyakit Dalam Fakultas Kedokteran Universitas Indonesia; 2008.

32. Sutedjo, SKM. Mengenal Penyakit Melalui Hasil Pemeriksaan Laboratorium. Yogyakarta: Aara Books; 2007.
Published
2022-04-29
How to Cite
SANDHIUTAMI, Ni Made Dwi et al. Safety Evaluation of Curcumin Nanoparticle Formula in Mice and Antioxidants Potency In-Vitro. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 20, n. 1, p. 63-72, apr. 2022. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1187>. Date accessed: 21 nov. 2024. doi: https://doi.org/10.35814/jifi.v20i1.1187.
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Articles