Isolasi dan Identifikasi Senyawa (-)-Asam Usnat dari Lichen Usnea sp. serta Aktivitas Sitotoksiknya terhadap Sel Murine Leukemia P388

  • MAULIDIYAH MAULIDIYAH UNIVERSITAS HALU OLEO
  • THAMRIN AZIS UNIVERSITAS HALU OLEO
  • SITTI HADIJAH SABARWATI UNIVERSITAS HALU OLEO
  • MUHAMMAD NURDIN UNIVERSITAS HALU OLEO

Abstrak

Lichen adalah organisme simbiosis antara ganggang dan jamur yang diketahui menghasilkan metabolit sekunder yang khas dan memiliki bioaktivitas. Senyawa golongan depsida, desidon dan dibenzofuran adalah kelompok senyawa yang banyak dijumpai dalam Lichen. Penelitian ini bertujuan untuk mengisolasi dan menentukan struktur kimia senyawa metabolit sekunder dari Lichen Usnea sp.  dan menguji aktivitas sitotoksiknya terhadap sel murine leukemia P388. Isolasi dilakukan dengan teknik kromatografi kolom menggunakan fase diam silika gel 60 dengan eluen campuran n-heksana dan etil asetat secara elusi gradien. Senyawa yang diperoleh diidentifikasi menggunakan spektroskopi UV-Vis, IR, 1H-NMR, 13C-NMR. Hasil identifikasi isolat diperoleh senyawa (-)-asam usnat dengan BM 344 g/ mol, berbentuk kristal jarum berwarna kuning. Hasil uji sitotoksik senyawa (-)-asam usnat terhadap sel murine leukemia P388 diperoleh nilai IC50 5,738 ± 0,61 µg/mL.

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MAULIDIYAH, MAULIDIYAH et al. Isolasi dan Identifikasi Senyawa (-)-Asam Usnat dari Lichen Usnea sp. serta Aktivitas Sitotoksiknya terhadap Sel Murine Leukemia P388. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 13, n. 1, p. 40-44, apr. 2015. ISSN 2614-6495. Tersedia pada: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/121>. Tanggal Akses: 09 aug. 2025
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