The Effects of Kaliputih Traditional Herbs on Fasting Blood Glucose Levels, SOD, HbA1c, Histopathology Pancreatic in Streptozotocin-Induced Diabetic Rats
Abstract
Prevention of DM by controlling sugar and lipid levels is the main therapy. The traditional medicine diabetes herb from Kaliputih Batur, Banjarnegara, Central Java consists of 11 kinds of medicinal plants that are empirically proven to be efficacious for diabetes mellitus. The potion is prepared by the infundation method. Three control groups (normal, positive, and negative) and three test groups with different doses (18, 36, and 54 mL/kg BW) that had previously been induced by streptozotocin were created using Sprague Dawley (SD) albino rats. Using HE staining, observations were obtained on fasting blood glucose (FBG), superoxide dismutase (SOD), HbA1c, and pancreatic organs. At doses of 36 and 54 mL/kg bw, results analysis of biochemical parameters of FBG levels revealed a substantial decline (P<0.05). At all dosages of the herb, there was a significant change in SOD activity (P<0.05), and at a dose of 18 mL/kg BW compared to negative control, there was a significant difference in HbA1c levels (P<0.05). HE staining results revealed no distinct
pathological alterations in pancreatic organ at the test dose compared to negative control with necrosis in the Langerhans insula. Herbal medicine for diabetes traditional medicine from Kaliputih Batur, Banjarnegara, Central Java has decreased blood glucose.
References
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33. Alshammari GM, Balakrishnan A, Al-Khalifa A. Antioxidant effect of Arabian coffee (Coffea arabica L) blended with cloves or cardamom in high-fat diet-fed C57BL/6J mice. Trop J Pharm Res. 2017;16(7):1545–52.
34. Katno, Pramono S. Level of Benefits and Safety of Medicinal Plants and Traditional Medicines. Tawangmangu Medicinal Plant Research Institute. 2008
35. Kondeti VK, Badri KR, Maddirala DR, Thur SKM, Fatima SS, Kasetti RB, et al. Effect of Pterocarpus santalinus bark, on blood glucose, serum lipids, plasma insulin and hepatic carbohydrate metabolic enzymes in streptozotocin-induced diabetic rats. Food Chem Toxicol an Int J Publ Br Ind Biol Res Assoc. 2010 May;48(5):1281–7.
36. Ramachandran S, Rajasekaran A, Adhirajan N. In vivo and in vitro antidiabetic activity of terminalia paniculata bark: an evaluation of possible phytoconstituents and mechanisms for blood glucose control in diabetes. ISRN Pharmacol. 2013;484675:1-10.
37. Sherwani SI, Khan HA, Ekhzaimy A, Masood A, Sakharkar MK. Significance of HbA1c Test in diagnosis and prognosis of diabetic patients. Biomark Insights. 2016;11:95–104.
38. Prameswari OM, Widjanarko SB. Test the effects of water extract of pandan wangi leaves on decreased blood glucose levels and histopathology of diabetic mice rats. J Pangan dan Agroindustri. 2014;2(2):16–27.
39. Pratiwi H, Winarso D, Handoyo N. Low density lipoprotein levels and histopathological features of the liver in type 1 diabetes mellitus model rats administered with curcuma ethanol extract. J Ilmu dan Teknol Has Ternak. 2016;11(1):1–7.
2. The Ministry of Health of the Republic of Indonesia. Basic health research. Riskesdas. 2018.
3. The Ministry of Health of the Republic of Indonesia. Riskesdas 2013; Expert Opinion on Investigational Drugs. 2013. p. 803–9.
4. The Ministry of Health of the Republic of Indonesia. Stay Productive, Prevent And Overcome Diabetes Mellitus. Data and Information Center of the Ministry of Health of the Republic of Indonesia. 2020.
5. Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress—A concise review. Saudi Pharmaceutical Journal. Elsevier B.V.; 2016; 24(5): 547–53.
6. Moussa S. Oxidative stress in diabetes mellitus. Rom J Biophys. 2008;18(3):225–36.
7. Jeffcoate SL. Diabetes control and complications: the role of glycated haemoglobin, 25 years on. Diabet Med. 2004 Jul;21(7):657–65.
8. Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature. 2000;408(6809):239–47. Available from: https://doi.org/10.1038/35041687
9. Murugan P, Pari L. Influence of tetrahydrocurcumin on erythrocyte membrane bound enzymes and antioxidant status in experimental type 2 diabetic rats. J Ethnopharmacol. 2007 Sep;113(3):479–86.
10. World Health Organization Fifty -Sixth World Health Assembly World Health Organization Fifty -Sixth. 2003;(May):19–28.
11. Center for Research and Development of Medicinal Plants and Traditional Medicines. National Report on Special Research Exploration of Community-Based Local Knowledge of Ethnomedicine and Medicinal Plants in Indonesia (RISTOJA) 2015. Center for Research and Development of Medicinal Plants. 2015;69. Available from: http://labdata.litbang.depkes.go.id/riset-badan-litbangkes/menu-riskesnas/menu-rikus/418-rikus-ristoja-2015
12. Nurraihana H, Norfarizan-Hanoon NA. Phytochemistry, pharmacology and toxicology properties of Strobilanthes crispus. International Food Research Journal. 2013; 20(5):2045-56.
13. Mohamed EAH, Yam MF, Ang LF, Mohamed AJ, Asmawi MZ. Antidiabetic properties and mechanism of action of Orthosiphon stamineus Benth Bioactive sub-fraction in streptozotocin-induced diabetic rats. JAMS J Acupunct Meridian Stud. 2013;6(1):31-40.
14. Boonphang O, Ontawong A, Pasachan T, Phatsara M, Duangjai A, Amornlerdpison D, et al. Antidiabetic and renoprotective effects of coffea arabica pulp aqueous extract through preserving organic cation transport system mediated oxidative stress pathway in experimental type 2 diabetic rats. Molecules. 2021 Mar;26(7):1907.
15. Zheng Y, Wang Q, Huang J, Fang D, Zhuang W, Luo X, et al. Hypoglycemic effect of dietary fibers from bamboo shoot shell: An in vitro and in vivo study. Food Chem Toxicol an Int J Publ Br Ind Biol Res Assoc. 2019 May;127:120–6.
16. Ji J, Yang X, Flavel M, Shields ZP-I, Kitchen B. Antioxidant and Anti-Diabetic Functions of a Polyphenol- Rich Sugarcane Extract. J Am Coll Nutr. 2019;38(8):670– 80.
17. Xie F, Gong S, Zhang W, Wu J, Wang Z. Potential of lignin from Canna edulis ker residue in the inhibition of α-D-glucosidase: Kinetics and interaction mechanism merging with docking simulation. Int J Biol Macromol. 2017;95:592–602.
18. Madihah M, Alfina F, Gani YY. Blood glucose level and pancreas histological section of mice (Mus musculus L.) induced by alloxan after treatment of Curcuma mangga val. rhizome extract. J Biol Udayana. 2016;20(2):64-8.
19. Ganugula R, Arora M, Jaisamut P, Wiwattanapatapee R, Jørgensen HG, Venkatpurwar VP, et al. Nano-curcumin safely prevents streptozotocin-induced inflammation and apoptosis in pancreatic beta cells for effective management of Type 1 diabetes mellitus. Br J Pharmacol. 2017 Jul;174(13):2074–84.
20. Santoso BSA, Sudarsono, Nugroho AE, Murti YB. Hypoglycemic activity and pancreas protection of combination of Morinda citrifolia Linn. juice and Curcuma xanthorrhiza Roxb. juice on streptozotocin-induced diabetic rats. Indones J Pharm. 2018;29(1):16–22.
21. Kurup SB, Mini S. Averrhoa bilimbi fruits attenuate hyperglycemia-mediated oxidative stress in streptozotocin-induced diabetic rats. J Food Drug Anal. 2017;25(2):360–8. Available from: https://www.sciencedirect.com/science/ article/pii/S1021949816300886
22. Chahyadi A, Hartati R, Wirasutisna K, Yaman E. Boesenbergia Pandurata Roxb., an indonesian medicinal plant: phytochemistry, biological activity, Plant Biotechnology. Procedia Chem. 2014;13:13–37.
23. Backer C., Brink RCB Van Den. Flora of Java (Spermatophytes only). Groningen: N.V.P. Noordhoff; 1965.
24. Steenis Hamzah, Amir, Toha, Moehamad., CGGJ van. The mountain flora of Java. Leiden; Boston: Brill; 1958. Available from: http://site.ebrary.com/id/10234955
25. Minister of Health of the Republic of Indonesia. Indonesian Traditional Medicine Formularium. Decree Of The Minister Of Health Of The Republic Of Indonesia; 2017 p. 1–135.
26. Nugroho AE. Review : Animal Models Of Diabetes Mellitus : Pathology And Mechanism Of Some Diabetogenics. Biodiversitas J Biol Divers. 2006;7(4):378–82.
27. Bourne, Zastrow. Receptors and Pharmacodynamics. In: Katzung, G B, editors. Basic and Clinical Pharmacology. Jakarta: Salemba Medika; 2001.
28. Tarigan IM, Bahri S, Saragih A. Antihyperuricemia activity of ethanol extract of herb (Peperomia pellucida (L.) Kunth) in male mice. J Pharm Pharmacol. 2013;1(1):37–43.
29. Den Hartogh DJ, Gabriel A, Tsiani E. Antidiabetic properties of curcumin ii: evidence from in vivo studies. Nutrients. 2019 Dec;12(1):1-27.
30. Raza H, John A. Streptozotocin-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in human hepatoma HepG2 cells. Int J Mol Sci. 2012;13(5):5751–67.
31. Erlidawati E, Safrida S, Mukhlis M. Antioxidant Potential As Antidiabetic. Syiah Kuala University Press. 2018;7(2):1–11.
32. Sunaryo H, Rahmania RA. Antioxidant activity of a combination of elephant ginger extract (Zingiber officinale rosc.) and zinc based on measurements of MDA, SOD, and catalase in hypercholesterolemia and hyperglycemia mice by inducing streptozotocin antioxidant activity of combination. J ilmu kefarmasian Indonesia. 2015;13(2):187–93.
33. Alshammari GM, Balakrishnan A, Al-Khalifa A. Antioxidant effect of Arabian coffee (Coffea arabica L) blended with cloves or cardamom in high-fat diet-fed C57BL/6J mice. Trop J Pharm Res. 2017;16(7):1545–52.
34. Katno, Pramono S. Level of Benefits and Safety of Medicinal Plants and Traditional Medicines. Tawangmangu Medicinal Plant Research Institute. 2008
35. Kondeti VK, Badri KR, Maddirala DR, Thur SKM, Fatima SS, Kasetti RB, et al. Effect of Pterocarpus santalinus bark, on blood glucose, serum lipids, plasma insulin and hepatic carbohydrate metabolic enzymes in streptozotocin-induced diabetic rats. Food Chem Toxicol an Int J Publ Br Ind Biol Res Assoc. 2010 May;48(5):1281–7.
36. Ramachandran S, Rajasekaran A, Adhirajan N. In vivo and in vitro antidiabetic activity of terminalia paniculata bark: an evaluation of possible phytoconstituents and mechanisms for blood glucose control in diabetes. ISRN Pharmacol. 2013;484675:1-10.
37. Sherwani SI, Khan HA, Ekhzaimy A, Masood A, Sakharkar MK. Significance of HbA1c Test in diagnosis and prognosis of diabetic patients. Biomark Insights. 2016;11:95–104.
38. Prameswari OM, Widjanarko SB. Test the effects of water extract of pandan wangi leaves on decreased blood glucose levels and histopathology of diabetic mice rats. J Pangan dan Agroindustri. 2014;2(2):16–27.
39. Pratiwi H, Winarso D, Handoyo N. Low density lipoprotein levels and histopathological features of the liver in type 1 diabetes mellitus model rats administered with curcuma ethanol extract. J Ilmu dan Teknol Has Ternak. 2016;11(1):1–7.
Published
2023-04-27
How to Cite
KUSUMO, Djati Wulan et al.
The Effects of Kaliputih Traditional Herbs on Fasting Blood Glucose Levels, SOD, HbA1c, Histopathology Pancreatic in Streptozotocin-Induced Diabetic Rats.
JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 21, n. 1, p. 9-16, apr. 2023.
ISSN 2614-6495.
Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1229>. Date accessed: 26 apr. 2024.
doi: https://doi.org/10.35814/jifi.v21i1.1229.
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