Antimicrobial activity and phytochemicals compound profile of ethanol extract moringa leaf (Moringa oleifera) compounds by FTIR
Abstract
Moringa plants are known in various parts of the world as a type of vegetable plant that is rich in nutrients and has multiple properties, one of which is as an antimicrobial. This research was conducted to test the antimicrobial inhibition of Moringa leaf decoction and ethanol extract against the growth of Escherichia coli and Aspergillus niger. The antimicrobial test in this study used the Disc diffusion method using various concentrations, including 25%, 50%, 75%, and 100%. There were two test samples, Moringa leaf decoction and ethanol extract using the maceration method with 96% ethanol solvent. In addition to the test group, there was also a control group, namely a positive control using chloramphenicol and ketoconazole antibiotics, negative control using aquadest as a decoction solvent, and DMSO as an extraction solvent. The ethanol extract was identified by the FTIR method. The results showed that Moringa leaf decoction and Moringa leaf ethanol extract could be used as antibacterial. Inhibition zones on Escherichia coli bacteria produced at concentrations of 25%, 50%, and 75% had a weak zone of inhibition category for both decoction and ethanol extract of Moringa leaves. Meanwhile, at a concentration of 100%, the decoction has a weak zone of inhibition and 100% ethanol extract of Moringa leaves can inhibit 8.25 mm which is included in the medium category. The inhibition zones of the fungus Aspergillus niger produced at concentrations of 25%, 50%, 75%, and 100% had no inhibition on both the decoction and ethanol extract of Moringa leaves. From the analysis of functional groups using the FTIR method, it can be predicted that in the Moringa leaf extract with methanol as solvent there are groups of phenolic compounds or flavonoids.
References
[2] Widiani PI, Pinatih KJP. Uji Daya Hambat Ekstrak Etanol Daun Kelor (Moringa oleifera) Terhadap Pertumbuhan Bakteri Methicillin Resistant Staphylococcus aureus (Mrsa). J Med Udayana. 2020;9(1):22–7.
[3] Zein U. Diare Akut Disebabkan Bakteri. Univ Stuttgart. 2004;(January 2004):1–15.
[4] Dima LLRH, Fatimawali, Lolo WA. Uji Aktivitas Antibakteri Ekstrak Daun Kelor ( Moringa oleifera L .) Terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Pharmacon J Ilm Farm – UNSRAT. 2016;5(2):282–9.
[5] Delina S, Arina Y, Sari NMI. Pengaruh Ekstrak Dan Fraksı Daun Afrıka (Vernonia amygdalina Delile) Terhadap Pertumbuhan Jamur Candida albicans Pendahuluan Penyakit infeksi masih menempati urutan tertinggi penyebab kesakitan dan kematian di negara-negara berkembang , termasuk Indones. J ‘Aisyiyah Med. 2019;4(3):1–15.
[6] Ariani N, Riski A. Aktivitas Ekstrak Etanol Kulit Buah Pisang Kepok Mentah (Musa paradisiaca forma typica) Terhadap Pertumbuhan Candida albicans Secara In Vitro. J Pharmascience. 2018;5(1):39–44.
[7] Purba EC. KELOR (Moringa oleifera Lam.): Pemanfaatan Dan Bıoaktıvıtas. Pro-Life. 2020;7(1):1–12.
[8] Anggi V, Tandi J, Veronika V. Total Flavonoıd Dan Efektıvıtas Ekstrak Etanol Bıjı Kelor (Moringa oleifera L) Asal Kota Palu Sulawesı Tengah Terhadap Hıstopatologı Pankreas Tıkus Putıh Jantan (Rattus norvegicus) Yang Dıınduksı Streptozotocın. J Ilm Manuntung. 2020;6(1):24.
[9] Mursito, S. MS, Lilies B, Mawaddah H. Ekstrak Daun Kelor (Moringa oleifera Lamk) dalam Menurunkan Kadar Glukosa Darah pada Tikus (Rattus novergicus). Bionature. 2020;21(1):6–12.
[10] Purwoko MLY, Syamsudin, Simanjutak P. Standardisasi Parameter Spesifik dan Nonspesifik Ekstrak
Etanol Daun Kelor (Moringa oleifera) Asal Kabupaten Blora. Sainstech Farma J Ilmu Kefarmasian. 2020;13(2):124–9.
[11] Ningsih AW, Pratama E, Komariyah S, Astuti DP, Klau ICS, Rahmawati D. Differences in extraction methods to antidiarrheal activity in vitro and in vivo in unripe Kayu banana fruit (Musa paradisiaca L. var. Kayu). Gac Méd Caracas. 2022;130(Supl 5):1133–46.
[12] Wijanarko A. Standardisasi simplisia daun ciplukan. J Farmasetis. 2020;9(1):31–40.
[13] Setiabudi DA, Tukiran. Uji Skrining Fitokimia Ekstrak Metanol Kulit Batang Tumbuhan Klampok Watu (Syzygium litorale). Unesa J Chem. 2017;6(3):157:155-160.
[14] Savitri E, Fakhrurrazi F, Harris A, Erina E, Sutriana A, Lubis TM. Uji Antibakteri Ekstrak Daun Kelor (Moringa oleifera L.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus (Antibacterial Activity Test of Moringa oleifera L. Extracts on Staphylococcus aureus). J Ilm Mhs Vet. 2018;2(3):373–9.
[15] Aziz Z, Yuliana ND, Simanjuntak P, Rafi M. FTIR and HPLC-Based Metabolomics of Yacon Leaves Extracts (Smallanthus sonchifolius [ Poepp & Endl .] H . Robinson ) from Two Locations in Indonesia. Indones J Chem. 2020;20(3):567–78.
[16] Al-Huqail AA, Behiry SI, Salem MZM, Ali HM, Siddiqui MH, Salem AZM. Antifungal, antibacterial, and antioxidant activities of Acacia saligna (Labill.) H. L. Wendl. Flower extract: HPLC analysis of phenolic and flavonoid compounds. Molecules. 2019;24(4).
[17] Rahmi N, Salim R, Miyono M, Rizki MI. Pengaruh Jenıs Pelarut Dan Metode Ekstraksı Terhadap Aktıvıtas Antıbakterı Dan Penghambatan Radıkal Bebas Ekstrak Kulıt Kayu Bangkal (Nauclea subdita). J Penelit Has Hutan. 2021;39(1):13–26.
[18] Retnowati Y, Bialangi N, Posagi NW. Pertumbuhan Bakteri Staphylococcus aureus pada Media yang Diekspos dengan Infus Daun Sambiloto (Andrographis paniculata). Saintek. 2011;6(2):397–405.
[19] Haryoto, D.S A. Aktivitas Farmakologi dan Kadar senyawa Fenolik Total dari Tanaman Andong Merah (Cordyline fruticosal. A. Chev.). J Urecol. 2021;1(1):743–52.
[20] Palupi IN. Daya Hambat Ekstrak Metanol Daun Sukun (Artocarpus altilis) Terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa. Fakultas Ilmu Keperawatan dan Kesehatan Universitas Muhammadiyah Semarang; 2016.
[21] Sulistiyono FD, Sofihidayati T, Lohitasari B. Uji Aktivitas Antibakteri dan Fitokimia kulit Bawang Merah (L.) Hasil Ekstraksi Metode Microwave Assisted Extraction (MAE). Mandala Heal a Sci J. 2018;11(2):71–9.
[22] Ibrahim A, Kuncoro H. Identifikasi Metabolit Sekunder dan Aktivitas Antibakteri Ekstak Daun Sungkai (Peronema canescens JACK.) Terhadap Beberapa Bakteri Patogen. J Trop Pharm Chem. 2012;2(1):8–18.
[23] Farmasi S, Mipa F. Ujı Aktıvıtas Antıdıare Ekstrak Etanol Batang Tıkus Putıh Jantan Wıstar Dıınduksı Bakterı Salmonella typhi Oleh : NYAYU JUWITA LESTARI. 2021.
[24] Wahyu ningsih A, Rochmanti M, Basori A. Efektıfıtas Antıdıareal Unrıpewooden Pısang (Musa paradisiaca L .). Fol Med Indones. 2020;56(3):208–15.
[25] Amalia A, Sari I, Nursanty R. Aktivitas Antibakteri Ekstrak Etil Asetat Daun Sembung (Blumeabalsamifera(L.) DC.) Terhadap Pertumbuhan Bakteri Methicillin Resistant Staphylococcus aureus(MRSA). Pros Semin Nas Biot. 2017;387–91.
[26] Normayunita S, Anam S, Khumaidi DA. Aktıvıtas Antıbakterı Fraksınası Ekstrak Kulıt Mentah Pısang Ambon (Musa paradisiaca var.sapientum) Terhadap Staphylococcus aureus Antibacterial Of Unripe Fruit Banana Peel (Musa paradisiaca var.sapientum) Fraction Againts Staphylococcus aureus. Online J Nat Sci. 2015;4(3):300–9.
[27] Priatna M. Pengaruh Rebusan Akar Bayam Durı (Amaranthus spinosus L.) Terhadap Dıare Pada Mencıt Jantan Galur Swıss Webster Dengan Metode Transıt Intestınal. J Kesehat Bakti Tunas Husada J Ilmu-ilmu Keperawatan, Anal Kesehat dan Farm. 2016;16(1):57.
[28] Handayani F, Warnida H, Nur SJ. Formulası Dan Ujı Aktıvıtas Antıbakterı Streptococcus mutans Darı Sedıaan Mouthwash Ekstrak Daun Salam (Syzygium polyanthum (Wight) Walp.). J Chem Inf Model. 2016;9(April):74–84.
[29] Prayoga, dkk. Antioksidan Ekstrak Kasar Daun Pepe ( Gymnema Reticulatum Br .) Pada Berbagai Jenis Pelarut. J Ilmu dan Teknol Pangan. 2019;8(2):111–21.
[30] Haryati D. Perbandingan Efek Ekstrak Buah Alpukat (Persea americana Mill) Terhadap Pertumbuhan Bakteri Pseudomonas aeruginosa dengan Metode Disk dan Sumuran. Pros Semin Nas Publ Hasil-Hasil Penelit dan Pengabdi Masy Univ Muhammadiyah Semarang. 2017;(September):348–52.
[31] Prayoga E. Perbandingan Efek Ekstrak Daun sirih hijau (Piper betle L.) dengan metode difusi disk dan sumuran terhadap pertumbuhan bakteri Staphylococcus aureus. Vol. 34, Fakultas Kedokteran dan Ilmu Kesehatan. Universitas Islam Negri Syarif Hidayatullah Jakarta; 2013.
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