The Characteristics of Some Commercial Arabica Coffee Beans in Indonesia

  • Yesi Desmiaty Fakultas Farmasi, Universitas Pancasila
  • Liliek Nurhidayati Fakultas Farmasi, Universitas Pancasila
  • Ni Made Dwi Sandhiutami Fakultas Farmasi, Universitas Pancasila
  • Reczky Muhammad Ramdhan Hasan Fakultas Farmasi, Universitas Pancasila
  • Kharinta Adella Meynderth Fakultas Farmasi, Universitas Pancasila
  • Dianita Ayu Noviastuti Fakultas Farmasi, Universitas Pancasila


Arabica coffee is one of the mainstay commodities in the Indonesian plantation sector. Geographical differences and the environment where coffee grow can affect the characteristic, phytochemical content and antioxidant activity of the beans. The aim of this study was to determine the phytochemical characteristics and antioxidant activity of 10 items of commercial Arabica coffee beans in Indonesia. The extraction of 10 coffee beans was carried out by kinetic maceration in 1 hour with 70% ethanol, then evaporated. Each extract was examined for morphology, phytochemical screening, assay of caffeine content using HPLC method, levels of flavan-3-ol, total flavonoid content (TFC), total polyphenolic content (TPC), antioxidant determination using DPPH and FRAP methods. All bean samples showed the presence of flavonoids, alkaloids, triterpenoids, coumarins, and tannins. The chemical content determination showed caff eine content of 1.5-2.9%; flavan-3-ol content 4.85-12.38cat/g extract; TFC 9.71–23.67QE/g extract; and TPC 21.8–76.26GAE/g extract. Tests of antioxidant capacity using the DPPH reveal an inhibiting effect with varying IC₅₀ values from 19.49-81.41g/mL and using the FRAP method gave IC₅₀ values from 18.01-61.2g/mL. This study indicates that coffee samples have the same phytochemical characteristics and also have an important antioxidant activity, which justifies its potential to be developed into antioxidant nutraceuticals.


1. Nkondjock A. Coffee consumption and the risk of cancer: An overview. Cancer Lett. 2009;277(2):121–5.

2. Micek A, Godos J, Lafranconi A, Marranzano M, Pajak A. Caffeinated and decaffeinated coffee consumption
and melanoma risk: a dose-response meta-analysis of prospective cohort studies. International Journal of Food Sciences and Nutrition. 2018;69(4):417-26.

3. Mills CE, Oruna-Concha MJ, Mottram DS, Gibson GR, Spencer JPE. The effect of processing on chlorogenic acid content of commercially available coffee. Food Chemistry. 2013;141(4):3335–40.

4. Watanabe T, Arai Y, Mitsui Y, Kusaura T, Okawa W, Kajihara Y, Saito I. The blood pressure-lowering
effect and safety of chlorogenic acid from green coffee bean extract in essential hypertension. Clinical and
experimental hypertension. 2006;28(5):439-49.

5. AL-Asmari KM, Zeid IMA, Al-Attar AM. Medicinal properties of arabica coff ee (Coffea arabica) Oil:
An Overview. Advancements in Life Sciences. 2020;8(1):20–9.

6. Del Rio D, Stalmach A, Calani L, Crozier A. Bioavailability of coffee chlorogenic acids and green
tea flavan-3-ols. Nutrients. 2010;2(8):820–33.

7. Alonso-Salces RM, Serra F, Remero F, Heberger K. Botanical and geographical characterization
of green coffee (Coffea arabica and Coffea canephora): chemometric evaluation of phenolic and methylxanthine contents. Journal of agricultural and food chemistry. 2009;57(10):4224–35.

8. Somporn C, Kamtuo A, Theerakulpisut P, Siriamornpun S. Effect of shading on yield, sugar content, phenolic
acids and antioxidant property of coffee beans (Coffea arabica L. cv. Catimor) harvested from northeastern
Thailand. Journal of the Science of Food and Agriculture. 2012;92(9):1956–63.

9. Farah A, Donangelo CM. Phenolic compounds in coffee. Brazilian journal of plant physiology.

10. Harborne A. Phytochemical methods a guide to modern techniques of plant analysis. 3rd ed.
Halesowen, United Kingdom: Springer Dordrecht; 1998

11. Bobo-García G, Davidov-Pardo G, Arroqui C, Vírseda P, Marín-Arroyo MR, Navarro M. Intralaboratory
validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. Journal of the Science of Food and Agriculture. 2015;95(1):204–9.

12. Desmiaty Y, Elya B, Saputri FC, Hanafi M, Prastiwi R. Antioxidant activity of Rubus fraxinifolius Poir.
and Rubus rosifolius J. Sm. Leaves. Journal of Young Pharmacists. 2018;10(2s):S93–6.

13. Hečimović I, Belščak-Cvitanović A, Horžić D, Komes D. Comparative study of polyphenols and caffeine
in different coffee varieties aff ected by the degree of roasting. Food Chemistry. 2011;129(3):991–1000.

14. Horszwald A, Andlauer W. Characterisation of bioactive compounds in berry juices by traditional
photometric and modern microplate methods. Journal of Berry Research. 2011;1(4):189–99.

15. Aprilia FR, Ayuliansari Y, Putri T, Azis MY, Camelina WD, Putra MR. Analisis kandungan kafein dalam kopi tradisional Gayo dan kopi Lombok menggunakan HPLC dan spektrofotometri UV/VIS. BIOTIKA Jurnal Ilmiah Biologi. 2018;16(2):40–4.

16. Affonso RCL, Voytena APL, Fanan S, Pitz H, Coelho DS, Horstmann AL, et al. Phytochemical composition,
antioxidant activity, and the eff ect of the aqueous extract of coffee (Coff ea arabica L.) bean residual press cake on the skin wound healing. Szymanska R, editor. Oxidative Medicine and Cellular Longevity. 2016;2016(1923754):1–10.

17. Cao H, Ou J, Chen L, Zhang Y, Szkudelski T, Delmas D, Daglia M, Xiao J. Dietary polyphenols and type 2 diabetes: Human Study and Clinical Trial. Critical reviews in food science and nutrition. 2019;59(20):3371-9.

18. Działo M, Mierziak J, Korzun U, Preisner M, Szopa J, Kulma A. The potential of plant phenolics in prevention and therapy of skin disorders. International Journal of Molecular Sciences. 2016;17(2):1-41.

19. Eun Lee K, Bharadwaj S, Yadava U, Gu Kang S. Evaluation of caffeine as inhibitor against collagenase,
elastase and tyrosinase using in silico and in vitro approach. Journal of enzyme inhibition and medicinal chemistry. 2019;34(1):927-36.

20. Pratami DK, Mun’im A, Sundowo A, Sahlan M. Phytochemical profile and antioxidant activity of
propolis ethanolic extract from Tetragonula bee. Pharmacognosy Journal. 2018;10(1):128-35.

21. Pratami DK, Desmiaty Y, Simorangkir EM, Faradhila D. Standardisasi dan uji aktivitas antioksidan ekstrak bahan alam propolis untuk terapi infeksi SARS-CoV2. Jurnal Ilmu Kefarmasian Indonesia. 2021;19(2):272-80
How to Cite
DESMIATY, Yesi et al. The Characteristics of Some Commercial Arabica Coffee Beans in Indonesia. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 20, n. 2, p. 245-251, oct. 2022. ISSN 2614-6495. Available at: <>. Date accessed: 08 feb. 2023. doi: