Molecular Mechanisms of Network Pharmacology-Based Immunomodulation of Huangqi (Astragali Radix)

  • Raymond Rubianto Tjandrawinata Fakultas Bioteknologi, Universitas Katolik Indonesia Atma Jaya
  • Anindini Winda Amalia Pengobatan Tradisional Tiongkok/Fakultas Kesehatan Institut Ilmu Kesehatan Bhakti Wiyata
  • Hartati Tuna Pengobatan Tradisional Tiongkok/Fakultas Kesehatan Institut Ilmu Kesehatan Bhakti Wiyata
  • Viedya Novalinda Said Pengobatan Tradisional Tiongkok/Fakultas Kesehatan Institut Ilmu Kesehatan Bhakti Wiyata
  • Santi Tan Dexa Laboratories of Biomolecular Sceinces, Dexa Group


Astragali Radix (Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao, also known as Huangqi) has been used to improve stamina and immunity for thousands of years. However, the bioactive components and their mechanisms are still unclear, limiting its clinical application. In this research, for the first time, network pharmacology was used to investigate the molecular mechanisms of Huangqi and its bioactive components that play an important role in improving the immune system. An amount of 1,430 immunomodulation-related target proteins was collected from the human genome database, with 171 target proteins from 20 bioactive components, and resulted in 60 intersection target proteins. Subsequently, the networks of common target and protein-protein interaction (PPI) were analyzed. The results showed that Huangqi influenced the modulations of lymphocytes (B cells and T cells), natural killer cells, macrophages, cytokines, immunoglobulins, and immune signal transduction. To a certain limit, this study revealed the potential bioactive components and pharmacological mechanisms of Huangqi in immunomodulation. It was able to direct novel drug development for treating immune system deficiency.


Schultze JL, Aschenbrenner AC. COVID-19 and the human innate immune system. Cell. 2021 Apr 1;184(7):1671–92.

2. Mak T, Saunders M, Jett B. Primer to the immune response - 2nd edition [Internet]. 2013 [cited 2022 Oct 24]. Available from:

3. Lesourd B. Nutritional factors and immunological ageing. Proceedings of the Nutrition Society. 2006 Aug;65(3):319–25.

4. Fu J, Wang Z, Huang L, Zheng S, Wang D, Chen S, et al. Review of the botanical characteristics, phytochemistry, and pharmacology of Astragalus membranaceus (Huangqi). Phytotherapy Research. 2014;28(9):1275–83.

5. Zhang N, Li J, Hu Y, Cheng G, Zhu X, Liu F, et al. Effects of Astragalus polysaccharide on the immune response to foot-and-mouth disease vaccine in mice. Carbohydrate Polymers. 2010 Oct 15;82(3):680–6.

6. Wang J, Jia J, Song L, Gong X, Xu J, Yang M, et al. Extraction, structure, and pharmacological activities of Astragalus polysaccharides. Applied Sciences. 2019 Jan;9(1):122.

7. Cho WCS, Leung KN. In vitro and in vivo immunomodulating and immunorestorative effects of Astragalus membranaceus. Journal of Ethnopharmacology. 2007 Aug 15;113(1):132–41.

8. Wu X, Wang S, Lu J, Jing Y, Li M, Cao J, et al. Seeing the unseen of Chinese herbal medicine processing (Paozhi): advances in new perspectives. Chin Med. 2018 Jan 17;13:4.

9. Li SP, Zhao XJ, Wang JY. Synergy of Astragalus polysaccharides and probiotics (Lactobacillus and Bacillus cereus) on immunity and intestinal microbiota in chicks. Poultry Science. 2009 Mar 1;88(3):519–25.

10. Shan J j, Wang Y, Weng Y q, Xie CY, Liu D, Hu ZB. Comparing compositions and immunoactivities of polysaccharide in hair root of Astragalus membranaceus and cultivated A. membranaceus. Chinese Traditional and Herbal Drugs. 2002;33(12):1096–8.

11. Li J, Zhong Y, Li H, Zhang N, Ma W, Cheng G, et al. Enhancement of Astragalus polysaccharide on the immune responses in pigs inoculated with foot-and-mouth disease virus vaccine. International Journal of Biological Macromolecules. 2011 Oct 1;49(3):362–8.

12. Chen Z, Liu L, Gao C, Chen W, Vong CT, Yao P, et al. Astragali Radix (Huangqi): a promising edible immunomodulatory herbal medicine. Journal of Ethnopharmacology. 2020 Aug 10;258:112895.

13. Berger SI, Iyengar R. Network analyses in systems pharmacology. Bioinformatics. 2009 Oct 1;25(19):2466–72.

14. Jin J, Chen B, Zhan X, Zhou Z, Liu H, Dong Y. Network pharmacology and molecular docking study on the mechanism of colorectal cancer treatment using Xiao-Chai-Hu-Tang. PLOS ONE. 2021 Jun 14;16(6):e0252508.

15. Ruswanto R, Rahayuningsih N, Hidayati NLD, Nuryani GS, Mardianingrum R. Uji in vitro dan studi in silico senyawa turunan n’-benzoylisonicotinohydrazide sebagai kandidat antituberkulosis. JURNAL ILMU KEFARMASIAN INDONESIA. 2019 Oct 29;17(2):218–26.

16. Zhou P, Zhou R, Min Y, An LP, Wang F, Du QY. Network pharmacology and molecular docking analysis on pharmacological mechanisms of Astragalus membranaceus in the treatment of gastric ulcer. Seidel V, editor. Evidence-Based Complementary and Alternative Medicine. 2022 Jan 31;2022:1–11.

17. Jia C, Pan X, Wang B, Wang P, Wang Y, Chen R. Mechanism prediction of Astragalus membranaceus against cisplatin-induced kidney damage by network pharmacology and molecular docking. Yuan Y, editor. Evidence-Based Complementary and Alternative Medicine. 2021 Aug 19;2021:1–15.

18. Zhang Y, Jiang W, Xia Q, Qi J, Cao M. Pharmacological mechanism of Astragalus and Angelica in the treatment of idiopathic pulmonary fibrosis based on network pharmacology. European Journal of Integrative Medicine. 2019 Dec;32:101003.

19. Wadhawan M, Chhabra V, Katiyar A, Sharma V, Khuntia BK, Rathore S, et al. A network pharmacology-based approach to explore therapeutic mechanism of Indian herbal formulation Nisha Amalaki in treating type 2 diabetes mellitus [Internet]. In Review; 2021 Oct [cited 2022 Jun 11]. Available from:

20. Rahman MdM, Vadrev SM, Magana-Mora A, Levman J, Soufan O. A novel graph mining approach to predict and evaluate food-drug interactions. Sci Rep. 2022 Dec;12(1):1061.

21. Li F, Duan J, Zhao M, Huang S, Mu F, Su J, et al. A network pharmacology approach to reveal the protective mechanism of Salvia miltiorrhiza-Dalbergia odorifera coupled-herbs on coronary heart disease. Sci Rep. 2019 Dec;9(1):19343.

22. Shahid M, Azfaralariff A, Law D, Najm AA, Sanusi SA, Lim SJ, et al. Comprehensive computational target fishing approach to identify Xanthorrhizol putative targets. Sci Rep. 2021 Dec;11(1):1594.

23. Zheng Y, Ren W, Zhang L, Zhang Y, Liu D, Liu Y. A review of the pharmacological action of Astragalus polysaccharide. Front Pharmacol. 2020 Mar 24;11:349.

24. Magee CN, Boenisch O, Najafian N. The role of costimulatory molecules in directing the functional differentiation of alloreactive T helper cells: role of costimulation in T cell differentiation. American Journal of Transplantation. 2012 Oct;12(10):2588–600.

25. Liu P, Zhao H, Luo Y. Anti-aging implications of Astragalus membranaceus (Huangqi): a well-known Chinese tonic. Aging and disease. 2017;8(6):868.

26. Huang H, Luo S, Huang D, Cheng S, Cao C, Chen G. Immunomodulatory activities of proteins from Astragalus membranaceus waste. J Sci Food Agric. 2019 Jun;99(8):4174–81.

27. 100 Astragalus membranaceus seeds - milkvetch perennial flowering herb Huangqi [Internet]. eBay. [cited 2022 Oct 25]. Available from:

28. Herbal medicine week: Astragalus and cancer [Internet]. Michael Thomsen. [cited 2022 Oct 25]. Available from:
How to Cite
TJANDRAWINATA, Raymond Rubianto et al. Molecular Mechanisms of Network Pharmacology-Based Immunomodulation of Huangqi (Astragali Radix). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 20, n. 2, p. 184-195, oct. 2022. ISSN 2614-6495. Available at: <>. Date accessed: 08 feb. 2023. doi: