Cytotoxic activity of isolate compounds from derendan (Lansium parasiticum (Osbeck) K.C. Sahni & Bennet) fruit peel

  • Haiyul Fadhli Sekolah Tinggi Ilmu Farmasi Riau, Pekanbaru, Riau, 28293, Indonesia
  • Wıky Rahmayanı Sekolah Tinggi Ilmu Farmasi Riau, Pekanbaru, Riau, 28293, Indonesia
  • Regina Allaya Sekolah Tinggi Ilmu Farmasi Riau, Pekanbaru, Riau, 28293, Indonesia.
  • Ikhsal Mukri Jumadila Sekolah Tinggi Ilmu Farmasi Riau, Pekanbaru, Riau, 28293, Indonesia.
  • Armon Fernando Sekolah Tinggi Ilmu Farmasi Riau, Pekanbaru, Riau, 28293, Indonesia
  • Rahayu Utami Sekolah Tinggi Ilmu Farmasi Riau, Pekanbaru, Riau, 28293, Indonesia

Abstract

Derendan (Lansium parasiticum (Osbeck) K.C. Sahni & Bennet) is a plant from the Meliaceae family that grows in the tropics, especially in Sumatra (Indonesia). It was reported that this plant has antimalarial, antimutagenic, and anticancer properties. This study aims to determine the cytotoxic activity of compounds isolated from the methanol extract of Lansium parasiticum bark. Extraction using maceration method with methanol solvent. Vacuum Liquid Chromatography (KVC) with 21 different eluent ratios (n-hexane : ethyl acetate and ethyl acetate ; methanol) was utilized for the fractionation. The result was 21 fractions. The washing technique was used to purify fraction 17. The obtained compound LPR17 with a melting point of 239-241ᵒC. Based on the UV spectrum, the wavelengths of the LPR17 compound were 277.0 nm and 205.0 nm. The FT-IR spectrum shows aliphatic OH, CH, C=O, C-H, and C-C functional groups. This compound reacts positively with the Liebermann-Burchard reagent. The compound obtained is a triterpenoid group. Cytotoxic test using BSLT method. LPR17 compounds provide very strong cytotoxic activity (LC50 = 67.15 g/mL).

References

[1] R. A. Syarif, A. R. Ahmad, A. Malik, U. M. Indonesia, J. Urip, and S. Km, “Identifikasi Golongan Senyawa Antioksidan Dengan Menggunakan Metode Perendaman Radikal DPPH Ekstrak Etanol Daun Cardia myxa L.,” J. Fitofarmaka Indones., vol. 2, no. 1, pp. 83–89, 2008.

[2] L. Hanum and R. S. Kasiamdari, “Tumbuhan Duku : Senyawa Bioaktif, Aktivitas Farmakologis dan Prospeknya dalam Bidang Kesehatan,” J. Biol. Papua, vol. 5, no. 2, pp. 84–93, 2013.

[3] S. Syamsuardi, C. Chairul, and P. Murni, “Analysis of Genetic Impurity of An Original Cultivar Duku (Lansium parasiticum (Osbeck.) KC Sahni Bennet.), from Jambi, Indonesia Using ITS and MatK Gene,” Int. J. Environ. Agric. Biotechnol., vol. 3, no. 2, p. 239084, 2018.

[4] S. M. Yermia, “Aktivitas Antioksidan dan Antikanker Ekstrak Kulit Batang Langsat (Lansium domesticum L.),” Institut Pertanian Bogor, 2008.

[5] I. Yunus, W. Boddhi, and E. De Queljoe, “Skrining Fitokimia dan Uji Toksisitas Ekstrak Etanol Daun Langsat (Lansium domesticum Corr) Terhadap Larva Artemia salina Leach dengan Metode Brine Shrimp Lethatily Test (BSLT),” Pharmacon, vol. 7, no. 3, pp. 89–96, 2018.

[6] P. Ramadhan, R., Worawalaii, W., Phuwapraisirisan, “New Onoceranoid Xyloside from Lansium parasiticum,” Nat. Prod. Res., vol. 33, no. 20, pp. 2917–2924, 2018.

[7] J. . Harbone, Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: Institusi Teknologi Bandung, 2006.

[8] M. S. Prasetyo and I. Entang, Pengelolaan Budidaya Tanaman Obat-obatan (Bahan Simplisia). Bengkulu: Badan Penerbitan Fakultas Pertanian UNIB, 2013.

[9] E. Hanani, Analisis Fitokimia. Jakarta: Buku Kedokteran EGC, 2015.

[10] A. Nugroho, Buku Ajar: Teknologi Bahan Alam. Banjarmasin: Universitas Lambung Mangkurat, 2017.

[11] A. N. Kristanti, Fitokimia. Surabaya: Airlangga University Press, 2019.

[12] P. M. S. Monk, Physical Chemistry: Understanding Our Chemical World. Inggris: John Wiley and Sons, 2008.

[13] S. Aldrich, HPLC Troubleshooting Guide, vol. 6628, no. 800. Bellefonte: Sigma-Aldrich, 2009.

[14] M. Sangi, M. R. J. Runtuwene, H. E. I. Simbala, and V. M. A. Makang, “Analisis Fitokimia Tumbuhan Obat di Kabupaten Minahasa Utara,” Chem. Prog., vol. 1, no. 1, pp. 47–53, 2008.

[15] W. A. E. Setyowati, S. R. D. Ariani, M. B. Ashadi, and C. P. Rahmawati, “Skrining Fitokimia dan Identifikasi Komponen Utama Ekstrak Metanol Kulit Durian (Durio zibethinus Murr.) Varietas Petruk,” in Seminar nasional kimia dan pendidikan kimia VI, 2014, pp. 271–280.

[16] Y. Kurniawan and M. Nur, “Studi Pemodelan Dinamika Proton Dalam Ikatan Hidrogen H2O,” Berk. Fis., vol. 8, no. 3, pp. 107–117, 2005.

[17] Effendy, Ikatan Kimia dan Kimia Organik Teori VSEPR Kepolaran dan Gaya Antar Molekul. Malang: Banyumedia Publising, 2006.

[18] M. Latief et al., “Isolasi Senyawa Triterpenoid Ekstrak Etanol Daun Jeruju (Achantus ilicifolius) dan Aktivitas Antibakterinya Terhadap S. aureus dan E. coli,” J. Kim. (Journal Chem., vol. 16, no. 1, pp. 35–44, 2022.

[19] S. Mulyanti, I. Musthapa, and S. Aisyah, “Isolasi dan Karakterisasi Senyawa Metabolit Sekunder dari Fraksi Aktif Antidiabeters Daging Buah Paria (Momordica charantia Linn),” J. Sains Dan Teknol. Kim., vol. 1, no. 2, pp. 1–9, 2010.

[20] M. Raineri, “Histochemical Localization of Chitin in Larvae of Artemia salina Leach (Phyllopoda),” Ital. J. Zool., vol. 4137, pp. 2–5, 2009, doi: 10.1080/11250008109439327.
Published
2024-04-30
How to Cite
FADHLI, Haiyul et al. Cytotoxic activity of isolate compounds from derendan (Lansium parasiticum (Osbeck) K.C. Sahni & Bennet) fruit peel. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 22, n. 1, p. 15-20, apr. 2024. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1309>. Date accessed: 21 june 2024. doi: https://doi.org/10.35814/jifi.v22i1.1309.