Correlation Analysis between Lymphocyte Proliferation Effect with Phenolic and Flavonoid Contents of Ethyl acetate Subfraction of Myrmecodia tuberosa (Non Jack) Bl. in BALB/C Mice, In Vitro
Abstract
Ethyl acetate fraction of Myrmecodia tuberosa (non Jack) Bl. expressed potential immunomodulatory effect in preliminary study. This study was aimed to determine the stimulation index of each subfraction towards lymphocyte of BALB/C mice by in vitro technique and to explore the activityof lymphocyte proliferation correlated to total phenolic contents. lymphocyte proliferation assay was performed by MTT. Determination of total flavanoid content was done by a colorimetric method while the total phenolic was tested by the Folin-Ciocalteu method. The result showed stimulation index profile was positively correlated with the total flavanoid content( R2 = 0,606) while weak correlation was observed on the total phenolic content (R2 = 0,167).
References
2. Nugroho I.A. Lokakarya Nasional Tanaman Obat Indonesia: Sinergi Multi Pihak dalam Budidaya, Pelestarian dan Peningkatan Kualitas Tanaman Obat
Genetic Resources Programme (APFORGEN), News Letter. Edisi 2. 2010. 1-4.
3. Sari LORK. Pemanfaatan obat tradisional dengan pertimbangan manfaat dan keamanannya. Majalah Ilmu Kefarmasian. 2006. 3(1):1-7.
4. Parinding Z. Potensi dan karakteristik bioekologis tumbuhan sarang semut di Taman Nasional Wasur Merauke Papua [tesis]. Bogor: Sekolah Pascasarjana Institut Pertanian Bogor; 2007.
5. Manoi F. Sarang semut (Myrmecodia) tanaman obat berpotensi menyembuhkan berbagai penyakit. Badan Penelitian dan Pengembangan Pertanian, Pusat Penelitian dan Pengembangan Perkebunan, Warta Penelitian dan Pengembangan Tanaman Industri. 2008. 14:26-30.
6. Alam S, Waluyo S. Sarang semut primadona baru dari Papua. Nirmala. Juli 2006. 76-8.
7. Hertiani T, Ediati S, Sumardi, Ulfah M. Preliminary study on immunomodulatory effect of sarang semut tubers Myrmecodia tuberosa and Myrmecodia pendens. On Line Journal of Biological Sciences. 2010. 10(3):136-41.
8. Sumardi. Efek ekstrak tuber sarang semut (Myrmecodia tuberosa (non Jack) Bl.) terhadap proliferasi sel TCD4+ dan TCD8 + Tikus Sprague Dawley yang diinduksi doksorubisin [tesis]. Yogyakarta: Fakultas Farmasi Universitas Gadjah Mada; 2011.
9. Muhtadi. Fraksinisasi dengan kromatografi vakum cair.Workshop : Isolasi Chemical Marker dari Bahan Alam dalam Rangka Milad Fakultas Farmasi Universitas Muhammadiyah Surakarta ke-10, Surakarta. 2009. 1-3.
10. Saraphanchotiwitthaya A, Ingkaninan K, Sripalakit P. Immuno-modulating activity of Thai rejuvenating plants. Naresuan University Journal. 2007. 15 (3):149-57.
11. Chun OK, Kim DO, Lee CY. Superoxide radical scavenging activity of the major polyphenols in fresh plums. J Agric Food Chem. 2003. 51:8067-72.
12. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002. 10:178-82.
13. menganalisis tumbuhan. Terbitan Kedua. Terjemahan Kosasih Padmawinata. Bandung: Penerbit ITB; 1987.
14. Walker EH, Pacold ME, Perisic O, Stephens L, Hawkins PT, Wymann MP, et al. Structural determinants of phosphoinositide 3-kinase inhibition by Wortmannin, LY294002, quercetin, myricetin and staurosporine. Mol Cell. 2000. 6:909-19. cit. Moskaug JO, Carlsen H, Myhrstad M, Blomhoff R. Molecular imaging of the biological effects of quercetin and ruercetin - Rich foods. Elsevier. 2004. 125:315-24. www.elsevier.com/ locate/mechagedev. doi : 10. 1016/j. mad. 2004.01.007. 15. Middleton E, Kandaswami C, Theoharides TC.
Implications for inflammation, heart disease and cancer. Pharmacol Rev. 2000. 52: 673-751. 16. Waterhouse AL. Current protocols in food analytical chemistry, Suppl
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