Correlation Analysis between Lymphocyte Proliferation Effect with Phenolic and Flavonoid Contents of Ethyl acetate Subfraction of Myrmecodia tuberosa (Non Jack) Bl. in BALB/C Mice, In Vitro

  • AKHMAD KHUMAIDI UNIVERSITAS TADULAKO
  • TRIANA HERTIANI UNIVERSITAS GADJAH MADA
  • EDIATI SASMITO UNIVERSITAS GADJAH MADA

Abstract

Ethyl acetate fraction of Myrmecodia tuberosa (non Jack) Bl. expressed potential immunomodulatory effect in preliminary study. This study was aimed to determine the stimulation index of each subfraction towards lymphocyte of BALB/C mice by in vitro technique and to explore the activityof lymphocyte proliferation correlated to total phenolic contents. lymphocyte proliferation assay was performed by MTT. Determination of total flavanoid content was done by a colorimetric method while the total phenolic was tested by the Folin-Ciocalteu method. The result showed stimulation index profile was positively correlated with the total flavanoid content( R2 = 0,606) while weak correlation was observed on the total phenolic content (R2 = 0,167).

References

1. Munawaroh E, Purwanto Y. Keanekaragaman Jenis Tumbuhan Liar Berguna dan Upaya Konservasi, Konferensi Nasional HIGI XV di UNS, Surakarta. 2001. 717-25.
2. Nugroho I.A. Lokakarya Nasional Tanaman Obat Indonesia: Sinergi Multi Pihak dalam Budidaya, Pelestarian dan Peningkatan Kualitas Tanaman Obat
Genetic Resources Programme (APFORGEN), News Letter. Edisi 2. 2010. 1-4.
3. Sari LORK. Pemanfaatan obat tradisional dengan pertimbangan manfaat dan keamanannya. Majalah Ilmu Kefarmasian. 2006. 3(1):1-7.
4. Parinding Z. Potensi dan karakteristik bioekologis tumbuhan sarang semut di Taman Nasional Wasur Merauke Papua [tesis]. Bogor: Sekolah Pascasarjana Institut Pertanian Bogor; 2007.
5. Manoi F. Sarang semut (Myrmecodia) tanaman obat berpotensi menyembuhkan berbagai penyakit. Badan Penelitian dan Pengembangan Pertanian, Pusat Penelitian dan Pengembangan Perkebunan, Warta Penelitian dan Pengembangan Tanaman Industri. 2008. 14:26-30.
6. Alam S, Waluyo S. Sarang semut primadona baru dari Papua. Nirmala. Juli 2006. 76-8.
7. Hertiani T, Ediati S, Sumardi, Ulfah M. Preliminary study on immunomodulatory effect of sarang semut tubers Myrmecodia tuberosa and Myrmecodia pendens. On Line Journal of Biological Sciences. 2010. 10(3):136-41.
8. Sumardi. Efek ekstrak tuber sarang semut (Myrmecodia tuberosa (non Jack) Bl.) terhadap proliferasi sel TCD4+ dan TCD8 + Tikus Sprague Dawley yang diinduksi doksorubisin [tesis]. Yogyakarta: Fakultas Farmasi Universitas Gadjah Mada; 2011.
9. Muhtadi. Fraksinisasi dengan kromatografi vakum cair.Workshop : Isolasi Chemical Marker dari Bahan Alam dalam Rangka Milad Fakultas Farmasi Universitas Muhammadiyah Surakarta ke-10, Surakarta. 2009. 1-3.
10. Saraphanchotiwitthaya A, Ingkaninan K, Sripalakit P. Immuno-modulating activity of Thai rejuvenating plants. Naresuan University Journal. 2007. 15 (3):149-57.
11. Chun OK, Kim DO, Lee CY. Superoxide radical scavenging activity of the major polyphenols in fresh plums. J Agric Food Chem. 2003. 51:8067-72.
12. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002. 10:178-82.
13. menganalisis tumbuhan. Terbitan Kedua. Terjemahan Kosasih Padmawinata. Bandung: Penerbit ITB; 1987.
14. Walker EH, Pacold ME, Perisic O, Stephens L, Hawkins PT, Wymann MP, et al. Structural determinants of phosphoinositide 3-kinase inhibition by Wortmannin, LY294002, quercetin, myricetin and staurosporine. Mol Cell. 2000. 6:909-19. cit. Moskaug JO, Carlsen H, Myhrstad M, Blomhoff R. Molecular imaging of the biological effects of quercetin and ruercetin - Rich foods. Elsevier. 2004. 125:315-24. www.elsevier.com/ locate/mechagedev. doi : 10. 1016/j. mad. 2004.01.007. 15. Middleton E, Kandaswami C, Theoharides TC.
Implications for inflammation, heart disease and cancer. Pharmacol Rev. 2000. 52: 673-751. 16. Waterhouse AL. Current protocols in food analytical chemistry, Suppl
Published
2017-11-30
How to Cite
KHUMAIDI, AKHMAD; HERTIANI, TRIANA; SASMITO, EDIATI. Correlation Analysis between Lymphocyte Proliferation Effect with Phenolic and Flavonoid Contents of Ethyl acetate Subfraction of Myrmecodia tuberosa (Non Jack) Bl. in BALB/C Mice, In Vitro. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 13, n. 1, p. 102-107, nov. 2017. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/132>. Date accessed: 23 nov. 2024.
Citation Formats
Issue
Vol 13 No 1 (2015): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.