The Combination of Colocasia esculenta L. and Zingiber officinale Potentially Inhibits Inflammation and Pain
Abstract
The active metabolites of Colocasia esculenta L. and Zingiber officinale L. have been reported to reduce pain and exert anti-inflammatory effects. This study aimed to examine the anti-inflammatory and analgesic effects of a combination of C. esculenta and Z. officinale extract. Thirty rats and mice were each divided into 6 groups (n=5), namely the normal group, negative control, positive control (Nadiclofenac), and 3 test groups were given a extract combination (dose 1.3 mg/20 gBW, 2.6 mg/20 gBW, and 5.2 mg/20 gBW). Winter's method was used for anti-inflammatory tests by inducing carrageenan in rat paws, and Sigmund's method was used for analgesic tests by the intraperitoneal induction of acetic acid in mice. The percentage of inhibition of leg edema in rats was 11.33%, 18.90%, and 19.10% for the three doses of the combined extracts of C. esculenta and Z. officinale, and 22.72% for Na-diclofenac (p<0.05). The percentage inhibition in the analgesic test in the positive control group and the three test groups was 61.17%, 41.19%, 51.79%, and 52.35%, respectively (p<0.05). The combination of the C. esculenta and Z. officinale extracts exhibited anti-inflammatory and analgesic effects. Dose of 2.6 mg/20 gBW and 5.2 mg/20 gBW as effective as Na-diclofenac.
References
1. Ferraz CR, Carvalho TT, Manchope MF, Artero NA, Rasquel-Oliveira FS, Fattori V, Casagrande R, Verri Jr WA. Therapeutic potential of flavonoids in pain and inflammation: mechanisms of action, pre-clinical and clinical data, and pharmaceutical development. Molecules. 2020; 10;25(3):762. Indonesia PR. Rekomendasi Perhimpunan Reumatologi Indonesia Untuk Diagnosis dan Pengelolaan Artritis Reumatoid. Jakarta: Perhimpunan Reumatologi Indonesia 2014;1–18.
2. Cui A, Li H, Wang D, Zhong J, Chen Y, Lu H. Global, regional prevalence, incidence and risk factors of knee osteoarthritis in populationbased studies. EClinicalMedicine 29--30: 100587. DOI: https://doi org/101016/j eclinm 2020Wilson, Patrick B.. Ginger (Zingiber officinale) as an Analgesic and Ergogenic Aid in Sport: A Systemic Review. Journal of Strength and Conditioning Research 2015 29(10):p 2980-2995.
3. Mostafa MN, Islam MH, Rahmatullah M. Antinociceptive Activity Evaluation Of Corms Of Colocasia Esculenta Var Esculenta. 2017.;
4. Prajapati R, Kalariya M, Umbarkar R, Parmar S, Sheth N, others. Colocasia esculenta: A potent indigenous plant. Int J Nutr Pharmacol Neurol Dis 2011;1(2):90.
5. Krishnapriya T V, Suganthi A. Biochemical and phytochemical analysis of Colocasia esculenta (L.) Schott tubers. International Journal of Research in Pharmacy and Pharmaceutical Sciences 2017;2(3):21–5.
6. Kravchenko I, Eberle L, Nesterkina M, Kobernik A. Anti-inflammatory and analgesic activity of ointment based on dense ginger extract (Zingiber officinale). Journal of Herbmed Pharmacology 2019;8(2):126–32.
7. Lahamendu B, Bodhi W, Siampa JP. Uji efek analgetik ekstrak etanol rimpang jahe putih (Zingiber officinale rosc. Var. Amarum) pada tikus putih jantan galur wistar (Rattus norvegicus). Pharmacon 2019;8(4):927–35.
8. Sandhiutami NMD, Khairani S, Dewi RS, Hakim ZR, Pradani AR. Anti-Inflammatory and Analgesic Activity of Musa balbisiana Peels In Vivo. Borneo Journal of Pharmacy 2022;5(2):81–92.
9. Mobasheri A, Fonseca JE, Gualillo O, Henrotin Y, Largo R, Herrero-Beaumont G, et al. Inflammation and Biomarkers in Osteoarthritis. Front Med (Lausanne) 2021;8.
10. Dzoyem JP, McGaw LJ, Kuete V, Bakowsky U. Anti-inflammatory and anti-nociceptive activities of African medicinal spices and vegetables. In: Medicinal spices and vegetables from Africa. Elsevier; 2017. page 239–70.
11. Mansouri MT, Hemmati AA, Naghizadeh B, Mard SA, Rezaie A, Ghorbanzadeh B. A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats. Indian J Pharmacol 2015;47(3):292.
12. Begum R, Sharma M, Pillai KK, Aeri V, Sheliya MA. Inhibitory effect of Careya arborea on inflammatory biomarkers in carrageenan-induced inflammation. Pharm Biol 2015;53(3):437–45.
13. Tsai DS, Huang MH, Tsai JC, Chang YS, Chiu YJ, Lin YC, et al. Analgesic and anti-inflammatory activities of Rosa taiwanensis nakai in mice. J Med Food 2015;18(5):592–600.
14. Kuedo Z, Sangsuriyawong A, Klaypradit W, Tipmanee V, Chonpathompikunlert P. Effects of astaxanthin from Litopenaeus vannamei on carrageenan-induced edema and pain behavior in mice. Molecules 2016;21(3):382.
15. Beatriz, Elizabeth R, Jose M, Manuel M, Daniel Z, Enrique P. Changes in saccharin preference behavior as the primary outcome to evaluate pain and analgesia in acetic acid induced visceral pain in mice. J Pain Res 2015;8:663–73.
16. Hmidani A, Bourkhis B, Khouya T, Ramchoun M, Filali-Zegzouti Y, Alem C, et al. Phenolic profile and anti-inflammatory activity of four Moroccan date (Phoenix dactylifera L.) seed varieties. Heliyon 2020;6(2):e03436.
17. Pawar HA, Choudhary PD, Kamat SR. An overview of traditionally used herb, Colocasia esculenta, as a phytomedicine. Med Aromat Plants 2018;7(02):1–7.
18. Duarte LJ, Chaves VC, dos Santos Nascimento MVP, Calvete E, Li M, Ciraolo E, et al. Molecular mechanism of action of Pelargonidin-3-O-glucoside, the main anthocyanin responsible for the anti-inflammatory effect of strawberry fruits. Food Chem 2018;247:56–65.
19. Jung H, Kwak HK, Hwang KT. Antioxidant and antiinflammatory activities of cyanidin-3-glucoside and cyanidin-3-rutinoside in hydrogen peroxide and lipopolysaccharide-treated RAW264. 7 cells. Food Sci Biotechnol 2014;23(6):2053–62.
20. Sun YAN, Li L. Cyanidin-3-glucoside inhibits inflammatory activities in human fibroblast-like synoviocytes and in mice with collagen-induced arthritis. Clin Exp Pharmacol Physiol 2018;45(10):1038–45.
21. Li Y, Zhao Y, Tan X, Liu J, Zhi Y, Yi L, et al. Isoorientin inhibits inflammation in macrophages and endotoxemia mice by regulating glycogen synthase kinase 3$β$. Mediators Inflamm 2020;2020.
22. De Sousa DP. Analgesic-like activity of essential oils constituents. Molecules 2011;16(3):2233–52.
23. de la Puente B, Romero-Alejo E, Vela JM, Merlos M, Zamanillo D, Portillo-Salido E. Changes in saccharin preference behavior as a primary outcome to evaluate pain and analgesia in acetic acid-induced visceral pain in mice. J Pain Res 2015;8:663.
24. Hassan FI, Zezi AU, Yaro AH, Danmalam UH. Analgesic, anti-inflammatory and antipyretic activities of the methanol leaf extract of Dalbergia saxatilis Hook. F in rats and mice. J Ethnopharmacol 2015;166:74–8.
25. Trongsakul S, Panthong A, Kanjanapothi D, Taesotikul T. The analgesic, antipyretic and anti-inflammatory activity of Diospyros variegata Kruz. J Ethnopharmacol 2003;85(2–3):221–5.
26. Adebayo AH, John-Africa LB, Agbafor AG, Omotosho OE, Mosaku TO, others. Anti-nociceptive and anti-inflammatory activities of extract of Anchomanes difformis in rats. Pak J Pharm Sci 2014;27(2):265–70.
27. Demsie DG, Yimer EM, Berhe AH, Altaye BM, Berhe DF. Anti-nociceptive and anti-inflammatory activities of crude root extract and solvent fractions of Cucumis ficifolius in mice model. J Pain Res 2019;12:1399.
28. Ong WY, Farooqui T, Ho CFY, Ng YK, Farooqui AA. Use of Phytochemicals against Neuroinflammation. Neuroprotective Effects Phytochem Neurol Disord 2017;1–41.
29. Guo D, Hu J. Spinal presynaptic inhibition in pain control. Neuroscience 2014;283:95–106.
30. Yang H, Woo J, Pae AN, Um MY, Cho NC, Park KD, et al. $α$-Pinene, a major constituent of pine tree oils, enhances non-rapid eye movement sleep in mice through GABAA-benzodiazepine receptors. Mol Pharmacol 2016;90(5):530–9.
2. Cui A, Li H, Wang D, Zhong J, Chen Y, Lu H. Global, regional prevalence, incidence and risk factors of knee osteoarthritis in populationbased studies. EClinicalMedicine 29--30: 100587. DOI: https://doi org/101016/j eclinm 2020Wilson, Patrick B.. Ginger (Zingiber officinale) as an Analgesic and Ergogenic Aid in Sport: A Systemic Review. Journal of Strength and Conditioning Research 2015 29(10):p 2980-2995.
3. Mostafa MN, Islam MH, Rahmatullah M. Antinociceptive Activity Evaluation Of Corms Of Colocasia Esculenta Var Esculenta. 2017.;
4. Prajapati R, Kalariya M, Umbarkar R, Parmar S, Sheth N, others. Colocasia esculenta: A potent indigenous plant. Int J Nutr Pharmacol Neurol Dis 2011;1(2):90.
5. Krishnapriya T V, Suganthi A. Biochemical and phytochemical analysis of Colocasia esculenta (L.) Schott tubers. International Journal of Research in Pharmacy and Pharmaceutical Sciences 2017;2(3):21–5.
6. Kravchenko I, Eberle L, Nesterkina M, Kobernik A. Anti-inflammatory and analgesic activity of ointment based on dense ginger extract (Zingiber officinale). Journal of Herbmed Pharmacology 2019;8(2):126–32.
7. Lahamendu B, Bodhi W, Siampa JP. Uji efek analgetik ekstrak etanol rimpang jahe putih (Zingiber officinale rosc. Var. Amarum) pada tikus putih jantan galur wistar (Rattus norvegicus). Pharmacon 2019;8(4):927–35.
8. Sandhiutami NMD, Khairani S, Dewi RS, Hakim ZR, Pradani AR. Anti-Inflammatory and Analgesic Activity of Musa balbisiana Peels In Vivo. Borneo Journal of Pharmacy 2022;5(2):81–92.
9. Mobasheri A, Fonseca JE, Gualillo O, Henrotin Y, Largo R, Herrero-Beaumont G, et al. Inflammation and Biomarkers in Osteoarthritis. Front Med (Lausanne) 2021;8.
10. Dzoyem JP, McGaw LJ, Kuete V, Bakowsky U. Anti-inflammatory and anti-nociceptive activities of African medicinal spices and vegetables. In: Medicinal spices and vegetables from Africa. Elsevier; 2017. page 239–70.
11. Mansouri MT, Hemmati AA, Naghizadeh B, Mard SA, Rezaie A, Ghorbanzadeh B. A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats. Indian J Pharmacol 2015;47(3):292.
12. Begum R, Sharma M, Pillai KK, Aeri V, Sheliya MA. Inhibitory effect of Careya arborea on inflammatory biomarkers in carrageenan-induced inflammation. Pharm Biol 2015;53(3):437–45.
13. Tsai DS, Huang MH, Tsai JC, Chang YS, Chiu YJ, Lin YC, et al. Analgesic and anti-inflammatory activities of Rosa taiwanensis nakai in mice. J Med Food 2015;18(5):592–600.
14. Kuedo Z, Sangsuriyawong A, Klaypradit W, Tipmanee V, Chonpathompikunlert P. Effects of astaxanthin from Litopenaeus vannamei on carrageenan-induced edema and pain behavior in mice. Molecules 2016;21(3):382.
15. Beatriz, Elizabeth R, Jose M, Manuel M, Daniel Z, Enrique P. Changes in saccharin preference behavior as the primary outcome to evaluate pain and analgesia in acetic acid induced visceral pain in mice. J Pain Res 2015;8:663–73.
16. Hmidani A, Bourkhis B, Khouya T, Ramchoun M, Filali-Zegzouti Y, Alem C, et al. Phenolic profile and anti-inflammatory activity of four Moroccan date (Phoenix dactylifera L.) seed varieties. Heliyon 2020;6(2):e03436.
17. Pawar HA, Choudhary PD, Kamat SR. An overview of traditionally used herb, Colocasia esculenta, as a phytomedicine. Med Aromat Plants 2018;7(02):1–7.
18. Duarte LJ, Chaves VC, dos Santos Nascimento MVP, Calvete E, Li M, Ciraolo E, et al. Molecular mechanism of action of Pelargonidin-3-O-glucoside, the main anthocyanin responsible for the anti-inflammatory effect of strawberry fruits. Food Chem 2018;247:56–65.
19. Jung H, Kwak HK, Hwang KT. Antioxidant and antiinflammatory activities of cyanidin-3-glucoside and cyanidin-3-rutinoside in hydrogen peroxide and lipopolysaccharide-treated RAW264. 7 cells. Food Sci Biotechnol 2014;23(6):2053–62.
20. Sun YAN, Li L. Cyanidin-3-glucoside inhibits inflammatory activities in human fibroblast-like synoviocytes and in mice with collagen-induced arthritis. Clin Exp Pharmacol Physiol 2018;45(10):1038–45.
21. Li Y, Zhao Y, Tan X, Liu J, Zhi Y, Yi L, et al. Isoorientin inhibits inflammation in macrophages and endotoxemia mice by regulating glycogen synthase kinase 3$β$. Mediators Inflamm 2020;2020.
22. De Sousa DP. Analgesic-like activity of essential oils constituents. Molecules 2011;16(3):2233–52.
23. de la Puente B, Romero-Alejo E, Vela JM, Merlos M, Zamanillo D, Portillo-Salido E. Changes in saccharin preference behavior as a primary outcome to evaluate pain and analgesia in acetic acid-induced visceral pain in mice. J Pain Res 2015;8:663.
24. Hassan FI, Zezi AU, Yaro AH, Danmalam UH. Analgesic, anti-inflammatory and antipyretic activities of the methanol leaf extract of Dalbergia saxatilis Hook. F in rats and mice. J Ethnopharmacol 2015;166:74–8.
25. Trongsakul S, Panthong A, Kanjanapothi D, Taesotikul T. The analgesic, antipyretic and anti-inflammatory activity of Diospyros variegata Kruz. J Ethnopharmacol 2003;85(2–3):221–5.
26. Adebayo AH, John-Africa LB, Agbafor AG, Omotosho OE, Mosaku TO, others. Anti-nociceptive and anti-inflammatory activities of extract of Anchomanes difformis in rats. Pak J Pharm Sci 2014;27(2):265–70.
27. Demsie DG, Yimer EM, Berhe AH, Altaye BM, Berhe DF. Anti-nociceptive and anti-inflammatory activities of crude root extract and solvent fractions of Cucumis ficifolius in mice model. J Pain Res 2019;12:1399.
28. Ong WY, Farooqui T, Ho CFY, Ng YK, Farooqui AA. Use of Phytochemicals against Neuroinflammation. Neuroprotective Effects Phytochem Neurol Disord 2017;1–41.
29. Guo D, Hu J. Spinal presynaptic inhibition in pain control. Neuroscience 2014;283:95–106.
30. Yang H, Woo J, Pae AN, Um MY, Cho NC, Park KD, et al. $α$-Pinene, a major constituent of pine tree oils, enhances non-rapid eye movement sleep in mice through GABAA-benzodiazepine receptors. Mol Pharmacol 2016;90(5):530–9.
Published
2023-04-30
How to Cite
SANDHIUTAMI, Ni Made Dwi et al.
The Combination of Colocasia esculenta L. and Zingiber officinale Potentially Inhibits Inflammation and Pain.
JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 21, n. 1, p. 81-89, apr. 2023.
ISSN 2614-6495.
Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1373>. Date accessed: 24 apr. 2024.
doi: https://doi.org/10.35814/jifi.v21i1.1373.
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