Penghambatan Aktivitas Enzim α-Glukosidase oleh Ekstrak Etanol Terstandar Daun Abelmoschus manihot (L.) Medik

  • Andri Prasetiyo Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta, 12640, Indonesia
  • Wiwi Winarti Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta, 12640, Indonesia
  • Rini Agustia Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta, 12640, Indonesia
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Abstrak

Daun gedi merah Abelmoschus manihot (L.) Medik telah banyak digunakan oleh masyarakat secara turun temurun sebagai obat antiabetes. Tujuan penelitian identifikasi golongan senyawa kimia, penetapan mutu dan uji aktivitas penghambat enzim α-glukosidase pada ekstrak. Metode ekstrak yang digunakan maserasi kinetik menggunakan pelarut etanol 70%, identifikasi dilakukan dengan penapisan fitokimia, penetapan mutu digunakan parameter spesifik dan non spesifik serta uji aktivitas antidiabetes dilakukan secara in vitro dengan penghambatan enzim α-glucosidase. Hasil uji skrining fitokimia menunjukkan adanya kandungan flavonoid, alkaloid, saponin, steroid, dan triterpenoid. Hasil uji parameter mutu diperoleh ekstrak kental, warna hijau kehitaman, kadar sari larut air 70,58%, kadar sari larut etanol 49,46%, susut pengeringan 7,82%, kadar air 4,19%, kadar abu total 6,97%, kadar abu tidak larut asam 0,60%, kadar abu larut air 5,64%, residu pelarut 0,76%, kadar logam berat Pb 1,35 mg/ kg, Cd 0,06 mg/kg, angka lempeng total ≤10 koloni/g, angka lempeng khamir 20,1995 koloni/g dan kadar flavonoid total 1,20%. Uji aktivitas penghambatan enzim α-glucosidase dari ekstrak dan akarbosa (kontrol positif) diperoleh nilai IC50 masing-masing sebesar 81,84 μg/mL dan 42,6 μg/mL. Ekstrak etanol daun A. manihot (L.) Medik memiliki potensi untuk menghambat enzim α-glukosidase.

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Diterbitkan
2023-10-16
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PRASETIYO, Andri; WINARTI, Wiwi; AGUSTIA, Rini. Penghambatan Aktivitas Enzim α-Glukosidase oleh Ekstrak Etanol Terstandar Daun Abelmoschus manihot (L.) Medik. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 21, n. 2, p. 159-164, oct. 2023. ISSN 2614-6495. Tersedia pada: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1387>. Tanggal Akses: 13 aug. 2025 doi: https://doi.org/10.35814/jifi.v21i2.1387.
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