Potential of The Tyrosinase Enzyme Inhibition by Standardized Ethanol Extract And Ethyl Acetate Fraction of Bengkoang Peel (Pachyrhizus erosus L.)

  • Zainur Rahman Hakim Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta 12640, Indonesia
  • Ratna Djamil Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta 12640, Indonesia
  • Novi Yantih Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta 12640, Indonesia
  • Ocitania Sanasih Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta 12640, Indonesia
  • Benni Iskandar School of Pharmacy, Faculty of Pharmacy, Taipei Medical University, No.250, Wuxing St., Xinyi Dist., Taipei City 110, Taiwan


This study examined the tyrosinase inhibitory potential of bengkoang peel standardised ethanolic extract and ethyl acetate fractions. 450 g of bengkoang peel powder (Pachyrhizus erosus L.) was macerated with 96% ethanol for 2 x 24 h, filtered, and concentrated till viscous. Extract standardisation uses specific and non-specific parameters. Microplate colorimetric tyrosinase inhibition assays were performed. The result of plant determination identified the bengkoang peel as Pachyrhizus erosus (L.) Urb. The organoleptic test revealed a brownish-yellow viscous extract, fresh smell, and bitter taste. Ethanol-soluble extract was 47.60% and water-soluble 22.42%. Bengkoang peel ethanolic extract includes alkaloids, flavonoids, saponins, tannins, and steroids. The non-specific parameters showed loss of drying (9.71%), water content (7.28%), total ash (9.91%), acid-insoluble ash (3.68%), no residual solvent content, lead content (-0.2324±0.1729), cadmium content (-0.0841±0.3418), TPC of 1 x 101 CFU/g, and TYMC of 2.26 x 102 CFU/g. The ethanol extract (103.9263) and ethyl acetate fraction (81.8606) IC50 values of bengkoang peel decrease tyrosinase enzyme activity compared to control (25.1235 ppm). These results show that the ethanol extract met both particular and non-specific quality standards. Bengkoang peel ethyl acetate inhibited tyrosinase.


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How to Cite
HAKIM, Zainur Rahman et al. Potential of The Tyrosinase Enzyme Inhibition by Standardized Ethanol Extract And Ethyl Acetate Fraction of Bengkoang Peel (Pachyrhizus erosus L.). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 21, n. 1, p. 145-152, apr. 2023. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1397>. Date accessed: 29 nov. 2023. doi: https://doi.org/10.35814/jifi.v21i1.1397.