Antimetabolic Syndrome Effect of 70% Ethanol Leaves Extract Ficus carica Linn. in Streptozotocin-Induced and High-Fat Diet Rats

  • Rahmatul Qodriah Doctoral Program, Faculty of Pharmacy, Universitas Pancasila, South Jakarta, DKI Jakarta 12640, Indonesia
  • Shirly Kumala Faculty of Pharmacy, Universitas Pancasila, South Jakarta, DKI Jakarta 12640, Indonesia
  • Syamsudin Syamsudin Faculty of Pharmacy, Universitas Pancasila, South Jakarta, DKI Jakarta 12640, Indonesia
  • Nancy Dewi Yuliana Department of Food Science and Technology, IPB University, Bogor, Indonesia; Halal Science Center, IPB University, Bogor, West Java, 16680, Indonesia
  • Vyacheslav Dushenkov International Research Training Center for Botanicals and Chronic Diseases (CBCD), Rutgers University, New Jersey, 08854, United States of America
  • Carrista Carrista Faculty of Pharmacy, Universitas Pancasila, South Jakarta, DKI Jakarta 12640, Indonesia
DOI: https://doi.org/10.35814/jifi.v21i1.1399

Abstract

Diabetes and obesity are risk factors for metabolic syndrome. Fig (Ficus carica L.) one of the plants is well known in traditional medicine system for their medicinal and therapeutic potentials. Fig leaves contain flavonoid and triterpenoid compounds which have antimetabolic effect. Streptozotocin is used to make diabetogenic rats to produce hyperglycemia conditions in test animals and high-fat diet to make rats model obesity. This study aimed to test the antidiabetic and antiobesity effect of 70% ethanol extract of fig leaves based on the parameters of measuring blood glucose levels with glucometer and Cholesterol strips. In this
study, rats were divided into 6 groups, namely normal, negative, positive control, and 3 control groups at 70%
ethanol extract dose level (250, 500, and 600 mg/kgBW) per oral. The measurement results in normal, negative, and positive control dose of 250, 500, 600 mg/kgBW at blood glucose levels were 105.8, 192, 134.2, 161, 157.75, 145.2 mg/dL. The conclusion of the results obtained was that 70% ethanol extract of fig leaves with a dose of 600 mg/kgBW can have an antidiabetic and obesity effect by lowering blood glucose levels as measured using glucometer, cholesterol strips and decreased body weight rats.

References

1. Çalişkan O, Aytekin Polat A. Phytochemical and antioxidant properties of selected fig (Ficus carica L.)
accessions from the eastern Mediterranean region of Turkey. Scientia Horticulturae. 2011 May;128(4):473–8.

2. Megdiche-Ksouri W, Trabelsi N, Mkadmini K, Bourgou S, Noumi A, Snoussi M, et al. Artemisia campestris phenolic compounds have antioxidant and antimicrobial activity. Ind Crops Prod 2015; 63:
104-13.

3. Perez C, Canal JR, Torres MD. Experimental diabetes treated with Ficus carica extract: effect on oxidative
stress parameters. Acta Diabetologica. 2003 Mar 1;40(1):3–8.

4. Zhuge F, Ni Y, Wan C, Liu F, Fu Z. Anti-diabetic effects of astaxanthin on an STZ-induced diabetic model in rats. Endocrine Journal. 2021;68(4):451–9.

5. Khan KY, Khan MA, Ahmad M, Hussain I, Mazari P, Fazal Hi, et al. Hypoglycemic potential of genus Ficus L.: A review of ten years of Plant Based Medicine used to cure Diabetes (2000-2010). Journal of Applied
Pharmaceutical Science. 2011 Aug 30;1(6):223–7.

6. Nugroho AE. Review : animal models of diabetes mellitus : pathology and mechanism of some diabetogenics. Biodiversitas Journal of Biological Diversity. 2006 Sep 26;7(4).

7. Furman B. Streptozotocin‐induced diabetic models in mice and rats. Current Protocols in Pharmacology
2015;5(47): 1-20.

8. Pandhare RB, Sangameswaran B, Mohite PB, Khanage SG. Antidiabetic activity of aqueous leaves extract of Sesbania sesban (L) Merr. in Streptozotocin induced diabetic rats. Avicenna J Med Biotechnol. 2011 Jan;3(1):37-43.

9. Latha M, Pari L. Effect of an aqueous extract of Scoparia dulcis on blood glucose, plasma insulin and
some polyol pathway enzymes in experimental rat diabetes. Braz J Med Biol Res. 2004;37(4):577-86.

10. Crisosto CH, Bremer V, Ferguson L, Crisosto GM. Evaluating quality attributes of four fresh fig (Ficus
carica L.) cultivars harvested at two maturity stages. HortScience. 2010 Apr;45(4):707–10.

11. Yazdanparast R, Esmaeili MA, Ashrafi JH. Teucrium polium extracts pancreatic function of streptozotocin
diabetic rats: a histological examination. Iran Biomed J. 2015;9: 81–85.

12. Kelley DE, Kuller LH, McKolanis TM, Harper P, Mancino J, Kalhan S. Effects of moderate weight loss and orlistat on insulin resistance, regional adiposity, and fatty acids in type 2 diabetes. Diabetes Care. 2014;27(1), 33-40.

13. Kumar S, Vasudena N, Sharma S. GC-MS analysis and screening of antidiabetic, antioxidant and hypolipidemic potential of Cinnamomum tamala oil in streptozotocin induced diabetes mellitus in rats. Cardiovas Diabetol. 2015;11:95-104.

14. Elbe H, Vardi N, Esrefoglu M, Ates B, Yologlu S, Taskapan C. Amelioration of streptozotocin-induced diabetic nephropathy by melatonin, quercetin, and resveratrol in rats. Hum Exp Toxicol. 2015;34:100–13.

15. Festing MF, Altman DG. Guidelines for the design and statistical analysis of experiments using laboratory animals. ILAR J 2002;43:244 58

16. Hao H, Liu J, Shen J, Zhao Y, Liu H, Hou Q, et al. Multiple intravenous infusions of bone marrow mesenchymal stem cells reverse hyperglycemia in experimental type 2 diabetes rats. Biochem Biophys Res Commun. 2013; 436:418–23.

17. Hall PM, Cook JGH, Sheldon J, Rutherford SM, Gould BJ. Glycosylated hemoglobin and glycosylated plasma proteins in the diagnosis of diabetes mellitus and impaired glucose tolerance. Diabetes Care 1984;7(2):147-150.

18. L J, Rains KS. Oxidative stress, insulin signaling and diabetes. Free Radic Bio Med. 2013;50(5):567-75. doi:10.1016/j.freeradbiomed.2010.12.006.

19. Janda E, Lascala A, Martino C, et al. PharmaNutrition Molecular mechanisms of lipid- and glucose-lowering
activities of bergamot flavonoids. Biochem Pharmacol. 2016;4:S8-S18. doi:10.1016/j.phanu.2016.05.001.
Published
2023-04-30
How to Cite
QODRIAH, Rahmatul et al. Antimetabolic Syndrome Effect of 70% Ethanol Leaves Extract Ficus carica Linn. in Streptozotocin-Induced and High-Fat Diet Rats. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 21, n. 1, p. 153-158, apr. 2023. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1399>. Date accessed: 25 apr. 2024. doi: https://doi.org/10.35814/jifi.v21i1.1399.
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Vol 21 No 1 (2023): JIFI
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