Development of Analytical Method of Amoxycillin Which is Selective and Not Interferred by Its Degradation Products

  • REHANA REHANA UNIVERSITAS JENDERAL SOEDIRMAN
  • HANIF HAFIIDH SETYO NUGROHO UNIVERSITAS JENDERAL SOEDIRMAN
  • VITIS VINI FERA RATNA UTAMI UNIVERSITAS JENDERAL SOEDIRMAN

Abstract

Assay of amoxycillin level in suspension form using spectrophotometry method at maximum wavelength around 291 nm is predicted to provide accurate and selective result because it is not interferred with its degradation products. This research aimed to obtain the predicted result. This research employed laboratory experimental method which included the process of measuring of maximum wavelength and operating time measurement, making of standard curve by measuring absorbance of amoxycillin standard solution at 40, 50, 80, 100 and 120 ppm at 290 nm and testing the selectivity and accuracy. Selectivity test was done by comparing UV spectrum and maximum absorbance of 1 mg/mL amoxycillin standard solution and 125 mg/5 mL amoxycillin suspension immediately after reconstitution with the one that was stored 9 days. Accuracy test was conducted by standard addition method using 1 mL amoxycillin suspension that was stored 9 days added by 1 mL 6260 ppm amoxycillin standard solution. Assay of amoxycillin on amoxycillin suspension by UV spectrophotometry at 290 nm using NaOH 0,1 N as solvent gave selective and accurate result with 97,36% recovery and it is not interfered with by its degradation product.

References

1. Setiabudy R. Antiobiotika golongan penisilin. Dalam : Gunawan SG. Farmakologi dan terapi. Edisi 5. Jakarta: Gaya Baru; 2007. 585-98.
2. Oricha BS and Hayyatu U. The pharmacokinetics of amoxycillin in healthy adult Nigerians. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2010.1(3):75-85.
3. Wahyono D, Indri H, dan Ika WBA. Pola pengobatan infeksi saluran pernapasan akut anak usia bawah lima tahun (balita) rawat jalan di puskesmas I Purwareja Klampok Kabupaten Banjarnegara tahun 2004. Majalah Farmasi Indonesia. 2008.19(1):20-4.
4. Utami VVFR dan Rehana. Stabilitas amoksisilin dalam sediaan sirup kering yang telah direkonstitusi. Prosiding Seminar Nasional Pengembangan Sumber Daya Pedesaan dan Kearifan Lokal Berkelanjutan. Purwokerto 23-24 November, 2011:101-8.
5. Pavia DL, Lampman GM, and Kriz GS. Introduction to spectroscopy. 3rd Ed. Australia: Thomson Learning; 2001. 14-51, 53-64.
6. Deshpande AD, Baheti KG, and Chatterjee NR. Degradation of β Lactam antibiotics. Current Science. 2004.87(12):1684-95.
7. Moffat CA, Osselton DM, Widdop B, Galichet YL. Clarke’s znalysis of drugs and poisons. 3rd Ed. London: Pharmaceutical Press; 2005. 612-44.
8. Anonim. Guidelines for single laboratory validation of chemical methods. Maryland: Association of Official Analytical Chemists; 2004. 16-7.
9. Unal K, Murat P, Elif K, and Feyyaz O. Spectrophotometric determination of amoxycillin in pharmaceutical formulations. Turk. J. Pharm. Sci. 2008.5(1):1-16.
10. Rohman A. Kimia farmasi analisis. Yogyakarta: Pustaka Pelajar; 2007. 220-61, 298-312.
11. Santjaka A. Biostatistik. Purwokerto: Global Internusa; 2008. 55-7.
12. Miller CJ, and Miller NJ. Statistics and chemometrics for analytical chemistry. 5th Ed. England: Pearson Educational Limited; 2005. 35-72.
13. Anonim. The merck index. 13th Ed. New Jersey: Merck&Co Inc; 2001. 5.
How to Cite
REHANA, REHANA; NUGROHO, HANIF HAFIIDH SETYO; UTAMI, VITIS VINI FERA RATNA. Development of Analytical Method of Amoxycillin Which is Selective and Not Interferred by Its Degradation Products. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 12, n. 2, p. 170-175, sep. 2014. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/143>. Date accessed: 25 apr. 2024.
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