Gelatin Analysis in Local Soft Candy Products using Fourier Transform Infrared (ATR-FTIR) Combined with Chemometrics

  • Nina Salamah Ahmad Dahlan Halal Center, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
  • Arum Fatmawati Departement of Analytical Chemistry, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
  • Any Guntarti Ahmad Dahlan Halal Center, Universitas Ahmad Dahlan, Yogyakarta, Indonesia


Gelatin is widely used in the food industry, including soft candy. It is generally sourced from cows and pigs. However, adding porcine gelatin to food will be problematic for the Muslim community due to its prohibition in their beliefs. Therefore, the soft candy’s components should be analysed to determine whether the functional groups are of bovine or porcine gelatin. From three local soft candy products in Indonesia, gelatin was isolated with acetone by vortexing and centrifugation. The supernatant was analysed with FTIR to determine its spectral profile. Furthermore, the wavenumbers and absorbances of gelatin’s functional groups were analysed in Minitab 18 with multivariate PLS and PCA. FTIR showed that gelatin contained proteins with O-H, aliphatic C-H, C=O, N-H, and C-N groups. PLS and PCA were conducted in the wavenumber range of 1576–1481 cm-1. The calibration yielded R2 = 0.9997 and RMSEC = 0.9376%. The internal validation showed R2 = 0.9998 and RMSECV = 1.29%, while the external validation produced R2 = 0.9996 and RMSEP = 1.04%. Clustering with PCA revealed that the gelatin sample from one soft candy was in the same quadrant as the reference bovine gelatin, while the other two were in different quadrants from both references.


1. Rather JA, Akhter N, Ashraf QS, Mir SA, Makroo HA, Majid D, Barba FJ, Khaneghah AM, Dar BN. A comprehensive review on gelatin: understanding impact of the sources, extraction methods, and modifications on potential packaging applications. Food Packaging and Shelf Life. 2022;34:100945.

2. Ramli SS, Nizar NN, Heng JY, Karde V, Abidin SA, Taib MN. Gelatin substitute. innovation of food products in halal supply chain worldwide. 2023:87-98.

3. Ali ME, Sultana S, Hamid SBA, Hossain MAM, Yehya WA, Kader MA, Bhargava SK. Gelatin controversies in food, pharmaceuticals, and personal care products: a uthentication methods, current status, and future challenges, Critical Reviews in Food Science and Nutrition. 2018; 58:1495–511.

4. Rohman A, Ghazali MAB, Windarsih A, Irnawati I, Riyanto S, Yusof FM, Mustafa S. Comprehensive review on application of FTIR spectroscopy coupled with chemometrics for authentication analysis of fats and oils in the food products. Molecules. 2020; 25(22): 5485.

5. Salamah N, Erwanto Y, Martono S, Rohman A. The employment of real-time polymerase chain reaction for the identification of bovine gelatin in gummy candy. Indonesian Journal of Pharmacy. 2022; 33(3):448–54.

6. Abd Mutalib S, Muin NM, Abdullah A, Hassan O, Mustapha WA, Sani NA, Maskat MY. Sensitivity of polymerase chain reaction (PCR)-southern hybridization and conventional PCR analysis for Halal authentication of gelatin capsules. LWT-Food Science and Technology. 2015 Sep 1;63(1):714-9.

7. Gunes R, Palabiyik I, Konar N, Said Toker O. Soft confectionery products: Quality parameters, interactions with processing and ingredients. Food Chemistry. 2022; 385: 132735.

8. Rahmawati A, Kuswandi B, Retnaningtyas Y. Detection of porcine gelatin in jelly soft candy sample using fourier transform infra red and chemometrics. e-Jurnal Pustaka Kesehatan. 2015; 3(2):278–83.

9. Fadlelmoula A, Pinho D, Carvalho VH, Catarino SO, Minas G. Fourier transform infrared (FTIR) spectroscopy to analyse human blood over the last 20 years: A review towards lab-on-a-chip. Micromachines. 2022; 13(2):1-20.

10. Fajriati I, Rosadi Y, Rosadi NN, Khamidinal K. Detec¬tion of animal fat mixtures in meatballs using fourier transform infrared spectroscopy (FTIR Spectroscopy). Indonesian Journal of Halal Research. 2021; 3(1):8–12.

11. Gontijo LC, Guimarães E, Mitsutake H, Santana FB, Santos DQ, Borges Neto W. Development and validation of PLS models for quantification of biodiesels content from waste frying oil in diesel by HATR-MIR. Revista Virtual de Química. 2014; 6: 1517–28.

12. Pimentel MF, Galvão RKH, De Araújo MCU. Guidelines for calibration in analytical chemistry. Part 2. Multispecies calibration. Quim. Nova. 2008;31:462-7.

13. Roggo Y, Chalus P, Maurer L, Lema-Martinez C, Edmond A, Jent N. A review of near infrared spectroscopy and chemometrics in pharmaceutical technologies. Journal Of Pharmaceutical and Biomedical Analysis. 2007;44(3):683-700.

14. Barak S, Mudgil D, Taneja S. Exudate gums: Chemistry, properties and food applications – a review. Journal of the Science of Food and Agriculture. 2020; 100(7):2828–35.

15. Zilhadia Z, Kusumaningrum F, Betha OS, Supandi S. Differentiation of bovine and porcine gelatin extracted from vitamin c gummy by combination method of fourier transform infrared (FTIR) and principal component analysis (PCA). Pharmaceutical Sciences and Research. 2018; 5(2): 90–6.

16. Hameed AM, Asiyanbi-H T, Idris M, Fadzillah N, Mirghani ME. A review of gelatin source authentication methods. Tropical Life Sciences Research. 2018 Jul;29(2):213-27.

17. Cebi N, Dogan CE, Mese AE, Ozdemir D, Arici M, Sagdic O. A rapid ATR-FTIR spectroscopic method for classification of gelatin gummy candies in relation to the gelatin source. Food Chemistry. 2019; 277: 373-81.

18. Cebi N, Durak MZ, Toker OS, Sagdic O, Arici M. An evaluation of fourier transform infrared spectroscopy method for the classification and discrimination of bovine, porcine and fish gelatins. Food Chemistry. 2016; 190:1109–15.

19. Jeevithan E. Bao B, Bu Y, Zhou Y, Zhao Q, Wu W. Type II collagen and gelatin from silvertip shark (Carcharhinus albimarginatus) cartilage: isolation, purification, physicochemical and antioxidant properties. Marine Drugs. 2014; 12: 3852–73.

20. Tiernan H, Byrne B, Kazarian SG. ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of bio-pharmaceuticals. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2020;241:118636.

21. Rohman A, Erwanto Y, Che YB. Analysis of pork adulteration in beef meatball using fourier transform infrared ( FTIR ) spectroscopy, MESC. 2011; 88: 91–5.

22. Roberts JJ, Cozzolino D. An overview on the application of chemometrics in food science and technology—An approach to quantitative data analysis. Food Analytical Methods. 2016;9(12):3258-67.

23. Hamid AH, Nurrulhidayah AF, Sani MSA, Muhammad NWF, Othman R, Rohman A. Discrimination of porcine and bovine gelatines based on reducing sugar types on Maillard reaction. Food Research. 2020; 4(2):301–6.
How to Cite
SALAMAH, Nina; FATMAWATI, Arum; GUNTARTI, Any. Gelatin Analysis in Local Soft Candy Products using Fourier Transform Infrared (ATR-FTIR) Combined with Chemometrics. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 21, n. 2, p. 239-246, oct. 2023. ISSN 2614-6495. Available at: <>. Date accessed: 25 may 2024. doi: