Characterization of JS-1 Isolate, an Alkalophylic Bacteria as Cyclodextrin Glycosyltransferase (CGTase) Producer from Sumedang, West Java

  • NUR MIFTAHURROHMAH UNIVERSITAS PANCASILA
  • MOORDIANI MOORDIANI UNIVERSITAS PANCASILA

Abstract

Cyclodextrin glycosyltransferase (CGTase) is an extracellular enzyme which is produced by some bacteria and archae that converts starch into cyclodextrin (CD). This enzyme has a high commercial value because of its product (CD) is widely used in several industries, such as pharmaceutical, chemistry, cosmetic, food, textile and also for environment protection. In Indonesia, there are still a few bacteria as CGTase producer were discovered and observed. The aim of this research was to find bacteria as CGTase producer from soil of corn farm land in Sumedang, West Java, and further characterized the isolate in produce CGTase. Horikoshi agar media was used as screening media. An alkalophylic bacteria as CGTase producer, JS-1 was successfully gained from this process. This isolate was characterized in the term of Gram staining, oxygen demand, motility and catalase test. The characterization results showed that JS-1 is a bacillus, Gram positive, facultative anaerob, motil and catalase positive. JS-1 isolate was also further characterized in order to determine optimum incubation period and temperature for CGTase production. The results were showed that JS-1 isolate was produced CGTase in maximum level if incubated for 48h at 37 °C.

References

1. Szejtli J. Past, present, and future of cyclodextrin research. Pure and Applied Chemistry. 2004. 76(10): 1825-45.
2. Yunianto P, Rahayu K, Wahyuntari B. Pengaruh pH dan suhu terhadap produksi b-siklodekstrin glikosiltrasferase (b-CGTase) oleh Bacillus sp. BK-1. Jurnal Sains dan Teknologi Indonesia. 2000. 2: 27-31.
3. Pribadi AF. Karakterisasi enzim CGTase dari isolat Bacillus sp. BGG-1 yang diisolasi dari taman rumput UNPAD berdasarkan parameter suhu dan pH [skripsi]. Bandung: Universitas Padjadjaran; 2001. Diambil dari pustaka.unpad.ac.id/archives/124079, diakses tanggal 6 Maret 2013.
4. Sahar LF. Karakterisasi enzim CGTase dari isolat Bacillus sp. yang diisolasi dari taman rumput UNPAD berdasarkan parameter suhu dan pH [skripsi]. Bandung: Universitas Padjadjaran; 2001. Diambil dari pustaka.unpad.ac.id/archives/124079, diakses tanggal 6 Maret 2013.
5. Sagita D. Isolat Indonesia LT1B dan PT2B penghasil siklodekstrin glukanotransferase (CGTase): Karakterisasi spesies, urutan gen cgtase dan profil enzim [tesis]. Bandung: Sekolah Farmasi ITB; 2011.
6. Rahman K, Illias RM, Hassan O, Mahmood NAN, Rashid NAA. Molecular cloning of a cyclodextrin glucanotransferase gene for alkalophilic Bacillus sp. TS1-1 and characterization of the recombinant enzyme. Enzyme and Microbial Technology. 2006. 39: 74-84.
7. Ramli N, Suraini AA, Hassan MA, Alitheen NB, Kamaruddin K, Ibrahim Z. Molecular cloning and extracellular expression of cyclodextrin glycosyltransferase from Bacillus sp. NR5UPM. African Journal of Microbiology Research. 2011. 5(21): 3475-82.
8. Berge O, Guinebretiere MH, Achouak W, Normand P, Heulin T. Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. Int J Syst Evol Microbiol. 2002. 52:607-16.
9. Takada M, Nakagawa Y, Yamamoto M. Biochemical and genetic analyses of a novel γ-cyclodextrin glucanotransferase from an alkalophilic Bacillus clarki 7364. Journal of Biochemistry. 2003. 133: 317-24.
10. Suraini AA, Sauvaphap AN, Osman H, Mohammed IAK, Norjahan BA, Kamarulzaman K, Rosli MI. A rapid screening method for CGTase-producing bacteria using different starches as carbon source. Malays Appl Biol. 2007. 36(2):1-5.
11. Park CS, Kwan HP, Seung HK. A rapid screening method for alkaline β-cyclodextrin glucanotransferase using phenolftalein-methyl orange containing-solid medium. Agricultural Biology and Chemistry. 1989. 53: 1167-9.
12. Cappucino JG, Sherman N. Microbiology a laboratory manual. 8th Ed. New York: Pearson Benjamin Cummings; 2008. 65-8, 79-81, 85-6, 147-70.
13. Pakzad SR, Ajdary SN, Moazami, Haghighi S. A novel method to detect β-cyclodextrin glucosyl transferase (β-CGTase) activity on polyacrylamide gels. Iranian Biomedicine Journal. 2004. 9: 87-90.
14. Goel A, Nene SN. Modifications in the phenolphthalein method for spectro-photometric estimation of beta cyclodextrin. Starch. 1995. 47: 399-400.
15. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey’s Manual of Determinative Bacteriology. 9th Ed. Philadelphia: Lippincott Williams & Wilkins; 1994. 559-60.
16. Qi Q, Zimmerman W. Cyclodextrin glucanotransferase: from gene to applications. Appl Microbiol Biotechnol. 2005. 66: 475-85.
17. More SS, Niraja R, Chris E, Akshata MB, Shetha V, Shubhrabarani DM. Isolation, purification and biochemical characterization of CGTase from Bacillus halodurans. Croatioan Journal of Food Technology, Biotechnology and Nutrition. 2012. 7(1-2): 90-7.
Published
2014-09-30
How to Cite
MIFTAHURROHMAH, NUR; MOORDIANI, MOORDIANI. Characterization of JS-1 Isolate, an Alkalophylic Bacteria as Cyclodextrin Glycosyltransferase (CGTase) Producer from Sumedang, West Java. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 12, n. 2, p. 216-221, sep. 2014. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/153>. Date accessed: 05 nov. 2024.
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Vol 12 No 2 (2014): JIFI
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