α-Glukosidase Inhibitor Activity Test and Quality Test of Ethanolic Extract of Brotowali Stem (Tinospora crispa (L.) Miers.)

  • YESI DESMIATY UNIVERSITAS PANCASILA
  • RISMA MARISI TAMBUNAN UNIVERSITAS PANCASILA
  • KARTININGSIH KARTININGSIH UNIVERSITAS PANCASILA
  • LOLA DYAH PITHALOKA UNIVERSITAS PANCASILA

Abstract

In attempt to develop Indonesian traditional medicine (Jamu) to become standardize herbal, it is need to examine the extract that can guarantee its efficacy, quality and safety. Brotowali stem (Tinospora crispa (L.) Miers.) known has antidiabetic effect and no toxic activity. In this research, the  quality and α-glukosidase inhibitor activity of ethanolic extract of brotowali stem has been determined. In the quality test were determined specific parameters (identity, organoleptic, extractable matter), non specific parameters (loss on drying, water content, total ash content, solvent residue, heavy metal, and microbial contamination), quantification of markers content (total flavonoid content with spectrophotometric UV-Vis  and assay of apigenin content with densitometric method). The research showed that the extract was fulfiled the requirement, total flavonoid content was 0.52% and apigenin content was 0.03635%. The result of α-glukosidase inhibitor activity test gave IC50 of 237.26 ppm.

References

1. Sriyapai, Chutima. Hypoglycemic effect of Tinospora crispa dry powder in outpatients with metabolic syndrome at King Chulalongkorn Memorial Hospital. J Health Res. 2009. 23(3): 125-33.
2. Noor HA, Stephen JH. Antidiabetic effects of Tinospora crispa in rats. Journal of Ethnopharmacology. 1989. 27(1-2):149-61.
3. Noor HA, Stephen JH. Pharmacological characterization of the antihyperglycaemic properties of Tinospora crispa extract. Journal of Ethnopharmacology. 1998. 62(1): 7-13.
4. Badan POM RI. Monografi ekstrak tumbuhan obat Indonesia. Vol 2. Jakarta: 2006.22-8.
5. Departemen Kesehatan Indonesia. Materia Medika Indonesia. Jilid II. Jakarta: 1978. 91-95.
6. Departemen Kesehatan Republik Indonesia. Parameter standar umum ekstrak tumbuhan obat. Jakarta: Direktorat Jendral Pengawasan Obat Dan Makanan; 2000.
7. SK Dirjen POM No. 03726/B/SK/VII/89.
8. Rajalaksmi M, Eliza J, Priya CE, Nirmala, Daisy P. Anti-diabetic of Tinospora cordifolia stem extract on Streptozotocin- induced diabetic rats. African Journal of Pharmacy and Pharmacology. 2009. 3(5): 180-71.
Published
2014-09-30
How to Cite
DESMIATY, YESI et al. α-Glukosidase Inhibitor Activity Test and Quality Test of Ethanolic Extract of Brotowali Stem (Tinospora crispa (L.) Miers.). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 12, n. 2, p. 232-237, sep. 2014. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/157>. Date accessed: 25 apr. 2024.
Citation Formats
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Vol 12 No 2 (2014): JIFI
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Articles
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