Identification of Cytotoxic Compounds in Soft Coral Sarcophyton sp. from Panggang Island Water, Seribu Islands National Parks

SRI ISWANI; DUDI TOHIR; HEDI INDRA JANUAR

SRI ISWANI BOGOR AGRICULTURAL UNIVERSITY
DUDI TOHIR Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan
HEDI INDRA JANUAR Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Abstract

This research aimed to identify the cytotoxic compounds from Sarcophyton sp. which dominated the coral reefs area at Panggang Islands water, Seribu Islands National Parks. Preparative chromatography and bioassay MCF-7 cell lines guided fractionation had been used in isolation process of cytotoxic compounds. Moreover, UV-VIS, HR-ESI-MS, and 400 MHz NMR spectroscopy methods had been used as the technique to elucidated all of the active compound. The result of this study found three active cytotoxic compounds. These compounds were Sarcophytol (IC50 36,041 ppm), sarcophytol (IC50 71,03 ppm), and sarcophyhtolide (IC50 67,227 ppm). Ecologically, these results showed that Sarcophyton from Panggang Islands coral reefs waters produced a diversed bioactive compound so this organism can win the spatial competition, detterant predation, and dominate in this area.

References

1. Kelly SR, Jensen PR, Henkel TP, Fenical W, Pawlik JR. Effects of Caribbean sponge extracts on bacterial attachment. Aquat Microb Ecol. 2003.31:175-82.
2. Januar HI, Hendrarto B, Chasanah E, Wright AD. 2011. Nephthea sp.: Correlation between natural products production and pressure from local environmental stressors. J Mar Sci Res Dev. 2011.S8(001):1-6.
3. Sammarco PW, Coll JC. Chemical adaptations in the Octocorallia: evolutionary considerations. MarEcol Prog Ser. 1992.88:93-104.
4. Sotka E, Forbey J, Horn M, Poore A, Raubenheimer D. The emerging role of pharmacology in understanding consumer-prey interactions in marine and freshwater systems. Integr Comp Biol. 2009.49:291-313.
5. Changyun W, Haiyan L, Changlun S, Yanan W, Liang L, Huashi G. Chemical defensive substances of soft corals and gorgonians. Acta Ecologica Sinica. 2008. 28(5):2320-28.
6. Soedharma D, Kawaroe M, Haris A. Kajian Potensi Bioaktif Karang Lunak (Octorallia: Alcyonacea) Di Perairan Kepulauan Seribu, DKI Jakarta. Jurnal Ilmu-ilmu Perairan dan Perikanan Indonesia. 2005.12(2):121-8.
7. Setyaningsih I, Nurhayati T, Nugraha dan Gunawan I. Comparative Evaluation of The Antibacterial Activity of Soft Corals Collected from The Waters of Panggang Island, Kepulauan Seribu. Pharmacie Globale Int J Comprehensive Pharm. 2012.6(03):1-3.
8. Nurhayati T, Fikri M, dan Desniar. Aktivitas Inhibitor Protease dari Ekstrak Karang Lunak, Asal Perairan Pulau Panggang Kepulauan Seribu. Ilmu Kelautan. 2010.15(2):59-65.
9. Meyer BN, Ferrigni NR., Putnam JE, Jacobson LB, Nichols DE, McLaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Medica. 1982.45:31-4.
10. Koop K, Booth D, Broadbent A, Brodie J, Bucher D, Capode D, et al. ENCORE: The effect of nutrient enrichment on coral reefs. Synthesis of results and conclusions. Mar Pollut Bull. 2001.42:91-120.
11. Pawlik JR. Marine invertebrate chemical defenses. Chem Rev. 1993.93:1911-22.
12. Hoover CA, Slattery M, Targett NM, Marsh AG. Transcriptome and metabolite responses to predation in a south pacific soft coral. Biol Bull. 2008.214: 319-28.
13. Fleury B, Coll JC, Tentori E, Duquesne S, Figueiredo L. Effect of nutrient enrichment on the complementary (secondary) metabolite composition of the soft coral Sarcophyton ehrenbergi (Cnidaria: Octocorallia: Alcyonacea) of the Great Barrier Reef. Mar Biol. 2000.136:63-8.
14. Estradivari, Syahrir M, Susilo N, Yusri S, Timotius S. Terumbu karang Jakarta - Laporan Pengamatan Jangka Panjang Terumbu karang kepulauan Seribu. Jakarta: Terangi – Yayasan Terumbu Karang Indonesia – The David and Lucile Packard Foundation; 2007.