Anticancer activity of Stichopus vastus extract against MCF-7 and HeLa cells from Natuna Waters

  • Mery Sukmiwati Universitas Riau
  • Nurjanah Nurjanah Bogor Agricultural Institute
  • Andarini Diharmi Universitas Riau
  • Indah Prasetya Putri University of Sumatra Utara
DOI: https://doi.org/10.35814/jifi.v23i1.1631

Abstract

Sea cucumbers can be explored as an alternative raw material by the pharmaceutical and fishery industries as anti-cancer agent because they contain potential bioactive compounds. The study aimed to determine the anti-cancer activity of  S. vastus extract against breast cancer cells (MCF-7) and uterine cancer cells (HeLa) using the MTT assay. The secondary metabolite content that found in S. vastus were steroid, saponin, and phenolic. TLC test results are indicated by Rf values, for saponins and steroid compounds in ethyl acetate extract are included in the standard Rf values. The IC50 values of the S. vastus extracts against MCF-7 and HeLa cells were determined using linear regression analysis against MCF-7 cells and HeLA cells,  the IC50 of methanolic extract were  17.25 μg/mL and 13.50 μg/mL, while the IC50 values of N-hexane fraction were 15.11 μg/mL and 10.09 µg/mL, which is considered as strong cytotoxicity (IC50 10-100 μg/mL), Ethyl acetate fraction had the lowest IC50 value on MCF-7 cells (13.32 µg/mL) and on HeLa cells (7.00 μg/mL), which is considered as strong cytotoxicity (IC50 10-100 μg/mL) and very strong cytotoxicity (IC50 < 10μg/mL). The ethyl acetate fraction of S. vastus showed better anticancer activity than methanol extract and n-hexane fraction. 

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Published
2025-04-30
How to Cite
SUKMIWATI, Mery et al. Anticancer activity of Stichopus vastus extract against MCF-7 and HeLa cells from Natuna Waters. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 23, n. 1, p. 99-112, apr. 2025. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1631>. Date accessed: 04 may 2025. doi: https://doi.org/10.35814/jifi.v23i1.1631.
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