Lelutung Tokak (Tabernaemontana macrocarpa Jack.) as Source of Bioactive Substances, Antioxidant and Anticancer

  • DJIHAN RYN PRATIWI INSTITUT PERTANIAN BOGOR
  • MARIA BINTANG INSTITUT PERTANIAN BOGOR
  • PARTOMUAN SIMANJUNTAK UNIVERSITAS PANCASILA

Abstract

This research was aimed to determine biologic activities of n-hexane, ethylacetate, ethanol and water extracts of lelutung tokak (Tabernaemontana macrocarpa Jack.) stem as antioxidant and anticancer. The result of this research showed that four extracts of T. Macrocarpa Jack. stem have antioxidation and anticancer activity. The antioxidant activity test with brine shrimp lethality test (BSLT) showed the LC50 value of n-hexane, ethylacetate, ethanol and water extracts of lelutung tokak stem were 567.89; 119.34; 120.56 and 156.44 ppm respectively; with DPPH method were 653.54; 48.10; 53.322 and 121.02 ppm, respectively. The anticancer test result showed that the IC50 of ethylacetate and ethanol extracts were 6.04 and 7.14 ppm, respectively. The phytochemical analysis result showed that four extracts of stem of T. macrocarpa Jack. preliminary contained alkaloid, flavonoid, saponin and tanin compounds. It was concluded that ethylacetate and ethanol extracts of stem of T. macrocarpa Jack. possess a powerful anticancer and antioxidation activities.

References

1. International Agency for Research on Cancer (IARC). Global Cancer facts & figures. 2nd Ed. Atlanta: American Cancer Society; 2011.
2. Badan Penelitian dan Pengembangan Kesehatan Kementrian Kesehatan RI. Riset kesehatan dasar 2013. Diambil dari http://www depkes.go.id. diakses tanggal 25 September 2014.
3. Wijayakusuma H. Atasi kanker dengan tanaman obat. Jakarta: Puspa Swara; 2006.
4. Patoppoi B. Pencegahan pengobatan penyakit kanker dengan keladi tikus. Jakarta: Prestasi Pustaka; 2012.
5. Lai CS, Mas RH, Nair NK, Majid MI, Mansor SM, Navaratnam V. Typhonium flagelliforme inhibits cancer cell growth in vitro and induces apoptosis: an evaluation by the bioactivity guided approach. 2008. 118(1):14-20.
6. Cochrane, Curtis BPK, Raveendran N, Steven JM, Anna PR, Cheppail R. Anticancer effect of Annona glabra plant extracts in human leukemia cell lines. Anticancer Research. 2008. 28:965-72.
7. Fitrya, Lenny A. Uji aktivitas antikanker secara in vitro dengan sel murine P-388 senyawa flavonoid dari fraksi etil asetat akar tumbuhan tunjuk langit (Helmynthostachis zeylanica (Linn) Hook). Jurnal Penelitian Sains. 2009. 12(1):12106.
8. Siddiuqui MJ, Ismail Z, Aisha AFA, Abdul AMS. Cytotoxic activity of Chatrantus roseus (Apocynaceae) crude extracts and pure compound against human colorrectal carcinoma cell line. International Journal of Pharmacology. 2010. 6(1):43-7.
9. Patel B, Sattwik D, Ravi P, Mohammad Y. Natural bioactive compound with anticancer potential. International Journal of Advances in Pharmaceutical Sciences. 2010. 1:32-41.
10. Mousinhoa, Kristiana C, Cecilia dCO, Jose RdO. Ferreira, Adriana AC. Antitumor effect of laticifer proteins of Himatanthus drasticus (Mart) Plumel Apocynaceae. Journal of Enthopharmacology. 2011.
11. Dey A. Alstonia scholaris R.Br. (Aponacynaceae): Phytochemistry and pharmacology: A concisse review. Journal of Applied Pharmaceutical Science. 2011. 01(06):51-7.
12. Bannwart G, Cecilia MAO, Lucilia K, Cleuza CDS, Ana LTG, Ruiz JEC et al. Antiproliferative activity and constituents of Aspidosperma macrocarpon (Apocynaceae) leaves. Rec Nat Prod. 2013. 7(2): 137-40.
13. Meyer BN, Ferrigni NR, Putnam JE, Jacobson LB, Nichols DE, McLaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituent. Planta Medica. 1982. 45:31-4.
14. Molyneux P. The use of stable free radical diphenylpicrylhyddrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Techol. 2004. 26(2):211-19.
15. Harborne JB. Metode fitokimia (Penuntun cara modern menganalisis tumbuhan). Terjemahan dari “Phytochemical methods” oleh K. Padmaminata. Bandung: Penerbit ITB; 1996.
16. Fransworth NR. Biological and phytochemical screenings of plant. J Pharm Sci. 1996. 55(3):225-65
Published
2014-09-30
How to Cite
PRATIWI, DJIHAN RYN; BINTANG, MARIA; SIMANJUNTAK, PARTOMUAN. Lelutung Tokak (Tabernaemontana macrocarpa Jack.) as Source of Bioactive Substances, Antioxidant and Anticancer. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 12, n. 2, p. 267-272, sep. 2014. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/164>. Date accessed: 05 nov. 2024.
Citation Formats
Issue
Vol 12 No 2 (2014): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.