Screening of Kapulaga, Temu putri and Senggugu Potencies for Inhibition of Biofilm Formation

  • TRIANA HERTIANI UNIVERSITAS GADJAH MADA
  • SINTAYU PUTRI WANDAN SARI UNIVERSITAS GADJAH MADA
  • FERRY RAHMA PUSPITA UNIVERSITAS GADJAH MADA
  • NURI IRIYANI UNIVERSITAS GADJAH MADA
  • SYLVIA UTAMI TUNJUNG PRATIWI UNIVERSITAS GADJAH MADA

Abstract

Kapulaga fruit, temu putri rhizome and sengugu bark have been reported as antibacterials, suggesting potency to be developed as antibiofilm agents. This research has investigated the inhibition activity on biofilm formation of Methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli. Following petroleum ether extraction, the remaining biomass was macerated in ethanol 70 %. After solvent evaporation, the ethanol extract was partitioned to yield hexane, ethyl acetate and water fractions. The inhibition activities of ethanol extracts, hexane, ethyl acetate and water fractions of samples were carried out in vitro on microplates flexible U-bottom 96 wells using crystal violet staining and were recorded at λ 595nm. Phytochemical identification were performed by thin layer chromatography. Results showed that extract or fraction samples which have the highest biofilm inhibition activity toward MRSA were hexane fraction of kapulaga IC50 0.23±0.01 mg/mL, temu putri IC50 0.45±0.03 mg/mL and the ethyl acetate fraction of senggugu IC50 0.35±0.022 mg/mL. Biofilm inhibition activity toward E. coli, were shown by the ethyl acetate fractions of kapulaga IC50 0.32±0.17 mg/mL, temu putri IC50 0.46±0.03 mg/mL and senggugu IC50 0.39±0.02 mg/mL.

References

1. Dewanti-Hariyadi R. Pembentukan biofilm bakteri pada permukaan padat. Buletin Teknologi dan Industri Pangan. 1997.8(1):70-6.
2. Lewis K. Riddle of biofilm resistance. Antimicrobial Agents and Chemotherapy. 2001.45(4):999-1007.
3. Coast J, Smith R, Miller M. Superbugs: Should antimicrobial resistance be included as a cost in economic evalution?. Health Economics. 1996. 5:217-26.
4. Quave CL, Plano LRW, Pantuso T, Bennett BC. Effects of extracts from Italian medicial plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus. Journal of Ethnopharmacology. 2008.118:418-28.
5. Brooks GF, Butel JS, Morse SA. Jawetz, Melnick, & Adelberg’s Mikrobiologi kedokteran. Diterjemahkan oleh Bagian Mikrobiologi Fakultas Kedokteran UNAIR. Jakarta: Salemba Medika; 2001. 235.
6. Cetin TE, Gurler N. Bacterilerin antibiyotiklere duyarlilik deneylerinin yapilmasi. Kukem Derg. 1989.12:2-3.
7. Agaoglu S, Dostbil N, and Alemder S. Antimicrobial effect of seed extract of Cardamom (Elettaria cardamomum Maton), YYU Vet Fak Derg. 2005. 16(2):99-101.
8. Gucin F, Dulger B, Ozbauram HC. Turkiye’de yetistirilen kultur mantari cesitlerinin antimikrobiyal, Aktivitesi X111, Ulusal Biyoloji Kongresi, Istanbul city, 2005.
9. Rakhmawati M. Daya antibakteri minyak atsiri rimpang kunci pepet (Kaempferia rotunda L.) terhadap Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 25922 serta uji toksisitas terhadap larva Artemia salina Leach [skripsi]. Yogyakarta: Fakultas Farmasi Universitas Gadjah Mada; 2001.
10. Ferawaty. Uji daya penghambatan ekstrak polar, semipolar dan non polar dari kulit batang senggugu (Clerodendrum serratum (L.) Moon.) terhadap kontraksi otot polos trakea marmut terisolasi karena pemberian histamin [skripsi]. Yogyakarta: Fakultas Farmasi Universitas Gadjah Mada; 2000.
11. Koo H, Hayacibara MF, Schobel BD, Cury JA, Rosalen PA, Park YK, et al. Inhibition of Streptococcus mutans biofilm accumulation and polysaccharide production by apigenin and tt-farnesol. Journal of Antimicrobial Chemotherapy. 2003.52:782-9.
12. Backer AC, Van Den Brink BCR. Flora of Java (Spermatophytes only). Vol II. Groningen: N.V.P. Noordhoff; 1965.
13. Departemen Kesehatan RI. Parameter standar umum ekstrak tumbuhan obat. Cetakan Pertama. Jakarta: Departemen Kesehatan RI; 2000. 9-11, 31.
14. Ando E, Monden K, Mitsuhata R, Kariyama R and Kumon H. Biofilm formation among methicillin-resistant Staphylococcus aureus isolates from patient with urinary tract infection. Acta Med. Okayama. 2004.58(4):207-14.
15. Stickler D, Dolman J, Chawla J. Activity of antiseptics against Escherichia coli growing as biofilms on silicone surfaces. Eur J Clin Microbiol Infect Dis. 1989.8(11):974-8.
16. Djordjevic D, Wiedmann M, McLandsborough LA. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. Applied and Environmental Microbiology. 2002.68(6):2950-8.
17. Hertiani T, Pratiwi SUT, Irianto IDK, Adityaningrum D, Pranoto B. Effect of Indonesian medicinal plants essential oils on Streptococcus mutans. Indonesian Journal of Pharmacy. 2011.22(3):174-81.
18. Jork H, Funk W, Fischer W, Wimmer H.Thin layer chromatography reagent and detection methods. Vol. 1. Weinheim: WCH; 1990.
19. Harborne JB. Phytochemical methods. A guide to modern techniques of plant analyses. 3rd ed. London: Chapman & Hall; 1998. 135-6.
20. Waksmundzka-Hajnos M, Sherma J, Kowalska T. Overview of the field of TLC in phytochemistry and the structure of the book. In: Waksmundzka-Hajnos M, Sherma J, Kowalska T. Thin Layer Chromatography in Phytochemistry. Bocaraton: CRC Press; 2008. 5-9.
21. Nobile CJ, Mitchel AP. Microbial biofilms: e pluribus unum. Current Biology. 2007.17(10):349.
22. Peeters E, Nelis HJ, Coenye T. Comparison of multiple methods for quantification of microbial biofilm grown in microtiter plates. Journal of Microbiological Methods. 2007.72:157-65.
23. Niu C, Gilbert ES. Colorimetric method for identifying plant essential oil components that affect biofilm formation and structure. Applied and Environmental Microbiology. 2004.12(70):6951-6.
24. Hentzer M, Givskov M. Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. J Clin Invest.
2002.112:1300-7
Published
2014-04-30
How to Cite
HERTIANI, TRIANA et al. Screening of Kapulaga, Temu putri and Senggugu Potencies for Inhibition of Biofilm Formation. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 12, n. 1, p. 17-24, apr. 2014. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/178>. Date accessed: 26 july 2024.
Citation Formats
Issue
Vol 12 No 1 (2014): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.