Screening of Kapulaga, Temu putri and Senggugu Potencies for Inhibition of Biofilm Formation
Abstract
Kapulaga fruit, temu putri rhizome and sengugu bark have been reported as antibacterials, suggesting potency to be developed as antibiofilm agents. This research has investigated the inhibition activity on biofilm formation of Methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli. Following petroleum ether extraction, the remaining biomass was macerated in ethanol 70 %. After solvent evaporation, the ethanol extract was partitioned to yield hexane, ethyl acetate and water fractions. The inhibition activities of ethanol extracts, hexane, ethyl acetate and water fractions of samples were carried out in vitro on microplates flexible U-bottom 96 wells using crystal violet staining and were recorded at λ 595nm. Phytochemical identification were performed by thin layer chromatography. Results showed that extract or fraction samples which have the highest biofilm inhibition activity toward MRSA were hexane fraction of kapulaga IC50 0.23±0.01 mg/mL, temu putri IC50 0.45±0.03 mg/mL and the ethyl acetate fraction of senggugu IC50 0.35±0.022 mg/mL. Biofilm inhibition activity toward E. coli, were shown by the ethyl acetate fractions of kapulaga IC50 0.32±0.17 mg/mL, temu putri IC50 0.46±0.03 mg/mL and senggugu IC50 0.39±0.02 mg/mL.
References
2. Lewis K. Riddle of biofilm resistance. Antimicrobial Agents and Chemotherapy. 2001.45(4):999-1007.
3. Coast J, Smith R, Miller M. Superbugs: Should antimicrobial resistance be included as a cost in economic evalution?. Health Economics. 1996. 5:217-26.
4. Quave CL, Plano LRW, Pantuso T, Bennett BC. Effects of extracts from Italian medicial plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus. Journal of Ethnopharmacology. 2008.118:418-28.
5. Brooks GF, Butel JS, Morse SA. Jawetz, Melnick, & Adelberg’s Mikrobiologi kedokteran. Diterjemahkan oleh Bagian Mikrobiologi Fakultas Kedokteran UNAIR. Jakarta: Salemba Medika; 2001. 235.
6. Cetin TE, Gurler N. Bacterilerin antibiyotiklere duyarlilik deneylerinin yapilmasi. Kukem Derg. 1989.12:2-3.
7. Agaoglu S, Dostbil N, and Alemder S. Antimicrobial effect of seed extract of Cardamom (Elettaria cardamomum Maton), YYU Vet Fak Derg. 2005. 16(2):99-101.
8. Gucin F, Dulger B, Ozbauram HC. Turkiye’de yetistirilen kultur mantari cesitlerinin antimikrobiyal, Aktivitesi X111, Ulusal Biyoloji Kongresi, Istanbul city, 2005.
9. Rakhmawati M. Daya antibakteri minyak atsiri rimpang kunci pepet (Kaempferia rotunda L.) terhadap Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 25922 serta uji toksisitas terhadap larva Artemia salina Leach [skripsi]. Yogyakarta: Fakultas Farmasi Universitas Gadjah Mada; 2001.
10. Ferawaty. Uji daya penghambatan ekstrak polar, semipolar dan non polar dari kulit batang senggugu (Clerodendrum serratum (L.) Moon.) terhadap kontraksi otot polos trakea marmut terisolasi karena pemberian histamin [skripsi]. Yogyakarta: Fakultas Farmasi Universitas Gadjah Mada; 2000.
11. Koo H, Hayacibara MF, Schobel BD, Cury JA, Rosalen PA, Park YK, et al. Inhibition of Streptococcus mutans biofilm accumulation and polysaccharide production by apigenin and tt-farnesol. Journal of Antimicrobial Chemotherapy. 2003.52:782-9.
12. Backer AC, Van Den Brink BCR. Flora of Java (Spermatophytes only). Vol II. Groningen: N.V.P. Noordhoff; 1965.
13. Departemen Kesehatan RI. Parameter standar umum ekstrak tumbuhan obat. Cetakan Pertama. Jakarta: Departemen Kesehatan RI; 2000. 9-11, 31.
14. Ando E, Monden K, Mitsuhata R, Kariyama R and Kumon H. Biofilm formation among methicillin-resistant Staphylococcus aureus isolates from patient with urinary tract infection. Acta Med. Okayama. 2004.58(4):207-14.
15. Stickler D, Dolman J, Chawla J. Activity of antiseptics against Escherichia coli growing as biofilms on silicone surfaces. Eur J Clin Microbiol Infect Dis. 1989.8(11):974-8.
16. Djordjevic D, Wiedmann M, McLandsborough LA. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. Applied and Environmental Microbiology. 2002.68(6):2950-8.
17. Hertiani T, Pratiwi SUT, Irianto IDK, Adityaningrum D, Pranoto B. Effect of Indonesian medicinal plants essential oils on Streptococcus mutans. Indonesian Journal of Pharmacy. 2011.22(3):174-81.
18. Jork H, Funk W, Fischer W, Wimmer H.Thin layer chromatography reagent and detection methods. Vol. 1. Weinheim: WCH; 1990.
19. Harborne JB. Phytochemical methods. A guide to modern techniques of plant analyses. 3rd ed. London: Chapman & Hall; 1998. 135-6.
20. Waksmundzka-Hajnos M, Sherma J, Kowalska T. Overview of the field of TLC in phytochemistry and the structure of the book. In: Waksmundzka-Hajnos M, Sherma J, Kowalska T. Thin Layer Chromatography in Phytochemistry. Bocaraton: CRC Press; 2008. 5-9.
21. Nobile CJ, Mitchel AP. Microbial biofilms: e pluribus unum. Current Biology. 2007.17(10):349.
22. Peeters E, Nelis HJ, Coenye T. Comparison of multiple methods for quantification of microbial biofilm grown in microtiter plates. Journal of Microbiological Methods. 2007.72:157-65.
23. Niu C, Gilbert ES. Colorimetric method for identifying plant essential oil components that affect biofilm formation and structure. Applied and Environmental Microbiology. 2004.12(70):6951-6.
24. Hentzer M, Givskov M. Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. J Clin Invest.
2002.112:1300-7
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