Effects of pH and Ionic Strength on the Solubility Profile of Ofloxacin

  • BUDIPRATIWI WISUDYA NINGSIH UNIVERSITAS JEMBER
  • SUWALDI SUWALDI UNIVERSITAS GADJAH MADA
  • AKHMAD KHARIS NUGROHO UNIVERSITAS GADJAH MADA

Abstract

Ofloxacin is an antibiotic and categorized as class 2 in Biopharmaceutics Classification System (BCS). Ofloxacin solubility is dependent on pH of the solvent due to its amphoteric properties. The objectives of this research was to investigate the effects of pH and ionic strength on solubility profile of ofloxacin. Ofloxacin solubilities were measured at various pH ranged from 1.5 to 12.5 and ionic strengths from 0.01 to 0.20 respectively. Solubility experiments were carried out using shaking thermostatic-waterbath for 3 hours at a temperature of 37°C ± 1°C. Results showed that ofloxacin solubility increased at pH’s below the pKa1 value (6.08) and above the pKa2 value (8.25). However, the solubility decreased at pH’s between pKa1 and pKa2. Changing the solution pH from 6.2 to 1.5 or to 12.5 affected the solubility of ofloxacin increased about 9.96 and 8.64 times higher, respectively. Ofloxacin solubility in various ionic strengths showed that stronger ionic strength solution caused an increase in ofloxacin solubility. This mean that there was a salting in phenomena. Least-Square Fitting Analysis using Henderson-Hasselbalch equation for amphoteric compound was insufficient to describe ofloxacin solubility process in a series of pH.

References

1. Banker GS and Rhodes CT. Modern pharmaceutics. 4th Ed. New York: Marcel Dekker Inc.; 2002. 175-85.
2. Florence AT and Attwood D. Physicochemical principles of pharmacy. 4th Ed. Hongkong: MacMillan; 2006. 140-76.
3. Wu CY and Benet LZ. Predicting drug disposition via application of BCS: Transport/ absorption/ elimination interplay and development of a biopharmaceutics drug disposition classification system. Pharmaceutical Research. 2005. 22(1):11-23.
4. Okeri HA and Arhewoh IM. Analytical profile of the fluoroquinolone antibacterials I. Ofloxacin. Afr. J. Biotechnol. 2008. 7(6):670-80.
5. Ross DL and Riley CM. Dissociation and complexation of the fluoroquinolone antimicrobials - an update. J.Pharm. Biomed. Anal. 1994. 2(2):1325-31.
6. Sinko PJ. Martin’s physical pharmacy and pharmaceutical sciences. 5th Ed. Philadelphia: Lippincott Williams & Wilkins; 2006. 161-265.
7. Ross DL and Riley CM. Aqueous solubilities of some variously substituted quinolone antimicrobials. Int. J. Pharm. 1990. 63(3):237-50.
8. Okonogi S, Oguchi T, Yonemochi E, Puttipipatkhachorn S, Yamamoto K. Improved dissolution of ofloxacin via solid dispersion. Int. J. Pharm. 1997. 156(2):175-80.
9. Koester LS, Guterres SS, Le Roch M, Eifler-Lima VL, Zuanazzi JA, Bassani VL. Ofloxacin/beta-cyclodextrin complexation. Drug Dev. Ind. Pharm. 2001. 27(6): 533-40.
10. Park HR, Chung HC, Lee JK, and Bark KM. Ionization and divalent cation complexation of quinolones antibiotics in aqueous solution. Bull. Korean Chem. Soc. 2000. 21(9):849-53.
11. Supratman U. Elusidasi struktur senyawa organik. Bandung: Widya Padjajaran; 2010. 21-54.
12. Gupta PK. Solutions and phase equilibria in Remington, the science and practice of pharmacy. 20th Ed. Pennsylvania: Mack-Publishing Co.; 2000. 208-26.
13. Flor S. Pharmacokinetics of ofloxacin – an overview. Am. J. Med. 1989. 87(6):24S-30S.
14. Needham TE, Paruta AN, Gerraughty RJ. Solubility of amino acid in pure solvent system. J. Pharm. Sci. 1971. 60(4):565-7.
15. Pandit NK and Strykowsky JM. Effect of pH and temperature on the solubility of a surface active carboxylic acid. J. Pharm. Sci. 1989. 78(9):767-70.
16. Ross DL and Riley CM. Physicochemical properties of the fluoroquinolone antimicrobials II acid ionization constant and their relationship to structure. Int. J. Pharm. 1992. 83:267-72.
17. Goyne KW, Chorover J, Kubicki JD, Zimmermam AR. Absorption of the antibiotic ofloxacin to mesoporous and nonporous alumina and silica. J.Colloid Interface Sci. 2005. 283:106-70.
Published
2014-04-30
How to Cite
NINGSIH, BUDIPRATIWI WISUDYA; SUWALDI, SUWALDI; NUGROHO, AKHMAD KHARIS. Effects of pH and Ionic Strength on the Solubility Profile of Ofloxacin. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 12, n. 1, p. 25-31, apr. 2014. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/179>. Date accessed: 22 dec. 2024.
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Vol 12 No 1 (2014): JIFI
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