Accute Toxicity Assay, In Vitro Antioxidant Activity Test and Effect of Kemboja Merah (Plumeria rubra L.) Flowers Decoction on Malondialdehyde Leve

  • NI MADE DWI SANDHIUTAMI UNIVERSITAS PANCASILA
  • LESTARI RAHAYU UNIVERSITAS PANCASILA

Abstract

Antioxidants are substances that may inhibit free radical reactions in the body. The purpose of this research is to identify lethal dose, in vitro antioxidant activity and effect of kemboja merah flowers decoction (Plumeria rubra L.) on MDA level. Lethal dose was measured by Weil method. Antioxidant activity assays was performed in vitro by DPPH method and in vivo by measuring MDA plasma level. The level of MDA was conducted using the thiobarbituric acid reactivity test. In the test, 30 mices were divided into 6 groups: normal, negative control, positive control and 3 group treated with variation dose of kemboja merah flowers decoction. LD50 of decoction kemboja merah flowers is > 15 g/kg of body weight. In vitro, antioxidant activity showed the IC50 value of decoction kemboja merah flowers is 36.96 μg/mL.The antioxidant activity of decoction kemboja merah flowers is smaller than vitamin C (IC50 5.89 μg/mL). In vivo antioxidant activity showed levels of MDA on normal group is 1.66 nmol/mL, negative control group is 5.67 nmol/mL, positive control group is 1.45 nmol/mL, treated group which was given by decoction kemboja merah flowers at dose 0.39 g/kg of body weight is 4.32

References

1. Hariana A. Tumbuhan obat dan khasiatnya. Seri 3. Jakarta: Penebar Swadaya; 2002. 7.
2. Clarkson PM, Thompson HS. Antioxidants: What role do they play in physical activity and health. Am J Clin Nutr. 2000. 72:637-46.
3. Allesio HM. Exercise-induced oxidative stress. Med Sci Sports Exerc. 1993. 25:218-24.
4. Viitala PE, Newhouse IJ, LaVoie N, Gottardo C. The effect of antioxidant vitamin supplementation on exercise induce lipid peroxidation in trained and untrained participants. Lipid in Health and Disease. 2004. 3:14.
5. Gutteridge JMC. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clinical Chemistry. 1995. 41(12):1819-28.
6. Murray RK, Granner D, Mayes PA, Rodwell VW. Harper’s Biochemistry. 5th ed. Appleton & Lange; 1996. 124, 156-7, 618-20, 730-1, 750, 798, 816.
7. Winarsi H. Antioksidan alami dan radikal bebas: potensi dan aplikasinya dalam kesehatan. Yogyakarta: Penerbit Kanisius; 2007.15-20, 79-81, 87, 97, 101, 137.
8. Tuminah S. Radikal bebas dan antioksidan.Cermin Dunia Kedokteran. 2000.128:49-51.
9. Gilman EF, Watson DG. Plumeria rubra Frangipani. Fact Sheet ST-490. Enviromental Holticulture Departement, Florida Cooperative Extension Service, Institute of Food and Agriculture Sciences, University of Florida. 1994.
10. Criley RA.Plumeria. Departement of Holticulture. College of Tropical Agriculture and Human Resources, University of Hawai at Manoa, Honolulu, Hawai. 1998.
11. Wrasiati LP, Triastuti IAA, Suhendra L. Antioxidant activity and quality characteristics of Frangipani tea produced at different drying temperature. Laporan penelitian hibah DIPAUniversitas Udayana, Denpasar. 2008. 34.
12. Sandhiutami NMD, Indrayani WAA. Uji aktivitas, kandungan fenolik total dan kandungan flavonoid total buah merah. Jurnal Ilmu Kefarmasian Indonesia. 2012.10(1):14.
13. Weil CS. Tables for convenient calculation of median- effectivity dose (LD50 or ED50) and Instructions Their Use.1952. 249-63.
14. Endrini S, Marsiati H, Suherman J, Fauziah O, Asmah R. Aktivitas antioksidan dan efek sitotoksik ekstrak kola (Cola nitida) pada kultur sel kanker hati (HepG-2). Jurnal Kedokteran Yarsi. 2009. 17(1):43
15. Jun, Fong X. Comparison of antioxidant activities of isoflavones from Kudzu Root (Pueraria labata Ohwl), J Food Sci. Institute of Technologist.2003. 68:2117-22.
16. Syamsuhidayat SS, Hutapea JR. Inventaris tanaman obat Indonesia. Jakarta: Penebar Swadaya; 1991.
19, 286-7.
17. Yuhernita. Analisis senyawa metabolit sekunder dari ekstrak metanol daun surian yang berpotensi sebagai antioksidan. Makara, Sains. 2011. 15(1):50-1.
18. Jawi IM, Suprapta DN, Arcana IN. Efek antioksidan ekstrak air umbi ubi jalar ungu (Ipomea batatas L.) terhadap darah dan berbagai organ pada mencit yang diberikan beban aktivitas fisik maksimal. Bali: Bagian Farmakologi Fakultas Kedokteran Universitas Udayana; 2000. 2.
19. Sirwandanu RH. Pengaruh pemberian superoksidadismutase terhadap perubahan kadar malondialdehid pada pasien hematoma epidural pasca operasi (disertasi). Jakarta: Fakultas Kedokteran Universitas Indonesia; 2009. 4,26.
Published
2014-04-30
How to Cite
SANDHIUTAMI, NI MADE DWI; RAHAYU, LESTARI. Accute Toxicity Assay, In Vitro Antioxidant Activity Test and Effect of Kemboja Merah (Plumeria rubra L.) Flowers Decoction on Malondialdehyde Leve. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 12, n. 1, p. 43-49, apr. 2014. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/182>. Date accessed: 24 apr. 2024.
Citation Formats
Issue
Vol 12 No 1 (2014): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.