Enhancement of 8-Hydroxy 9,12-Octadecadienoic Acid (8-HODE) Production by Endhophyitic Fungi Curvularia lunata BioMCC Fe-00283 Using Fermentation Medium Optimization

  • WAHONO SUMARYONO UNIVERSITAS PANCASILA
  • VINDYATRI WIDAYANTI UNIVERSITAS PANCASILA
  • ERWAHYUNI ENDANG PRABANDARI UNIVERSITAS PANCASILA

Abstract

8-Hydroxy 9,12-octadecadienoic acid (8-HODE) is an oxidized aliphatic fatty acid belongs to the group of oxylipins, which shows bioactivity. It was reported previously that Curvularia lunata BioMCC FE-00283, an endophytic fungi from Cibotium barometz be able to produce 8-HODE by submerged fermentation. Glucose, monosodium glutamate, corn oil are media components which showed significant effect toward HODE production by C. lunata BioMCC FE-00283. In this study we reported the enhancement of 8-HODE production by adding potato extract to previouse medium. These four medium components, which were potato extract, glucose, monosodium glutamate and corn oil, were optimized for 8-HODE production by Response Surface Metodology (RSM). A full factorial Central Composite Design (CCD) was used to explain the interaction effect between media components. Maximum HODE production predicted by the quadratic model was 16.46 mg/L where media composition consits of 36.50% potato extract, 3.60 g/L glucose, 15.92 g/L monosodium glutamate and 0.96 ml/L corn oil. Experimentally verification using these medium composition production of 8-HODE reached 16.189 ± 0.526 mg/L. This optimization,which was added potato extract as variabel in medium optimization was enhanced 3 times fold of 8-HODE concentration compare to the 3 variabel optimization.

References

1. Prabandari EE, Irawadi TT, Sumaryono W, Syamsu K. Citotoxic activity of active fraction and pure compound produced by endophytic fungi from Lombok Timur medicinal plants against breast cancer cell line MCF-7. J. Ilmu Kefarmasian Indonesia. 2012. 10(1):24-29.
2. Widayanti VT, Prabandari EE. Pengaruh varietas ekstrak kentang terhadap produksi 8-HODE oleh kapang endofit Curvularia lunata BioMCC FE-00283 (dalam proses publikasi).
3. Prabandari EE, Irawadi TT, Sumaryono W, Syamsu K. Optimation of hydorxy octadecadienoic acid (HODE) produce by endophytic fungi Curvularia lunata BioMCC FE-00283 using Response Surface Methodology. J. Ilmu Kefarmasian Indonesia. 2011.9 (2).
4. Tomita F. Endophytes in Southeast Asia and Japan: their taxonomic and potential applications. Fungal Diversity. 2003. 14:187–204.
5. Watanabe. Pictorial atlas of soil and seed fungi: Morphologies of cultured fungi and key to species. 2nd ed. Boca Raton: CRC Press; 2002. 237.
6. Bezerraa MA, Santelli RE, Oliveiraa EP, Villar LS, Escaleira LA. Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta. 2008. 76:965–77.
7. Prabandari EE, Irawadi TT, Sumaryono W, Syamsu K. Selection of carbon and nitrogen sources for 8-hydroxy-9,12-octadecadienoic acid production using endophytic fungi Curvularia lunata BioMCC FE-00283. Microbiology Indonesia. 2011. 5(4):187-91.
8. Bas D, Boyaci IH. Modeling and optimization I: Usability of response surface methodology. J. Food Engineer. 2007.78:836-45.
9. Weuster-Botz D. Experimental design for fermentation media development: Statistical design or global random search. J. Biosci. Bioeng. 2000.90(5):473-83.
10. Prabandari EE. Isolasi, karakterisasi dan optimasi medium produksi senyawa aktif kapang endofit untuk menghambat proliferasi sel kanker payudara MCF-7 secara in vitro [Disertasi]. Bogor: Institut Pertanian Bogor; 2012.
Published
2015-04-30
How to Cite
SUMARYONO, WAHONO; WIDAYANTI, VINDYATRI; PRABANDARI, ERWAHYUNI ENDANG. Enhancement of 8-Hydroxy 9,12-Octadecadienoic Acid (8-HODE) Production by Endhophyitic Fungi Curvularia lunata BioMCC Fe-00283 Using Fermentation Medium Optimization. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 12, n. 1, p. 67-74, apr. 2015. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/188>. Date accessed: 11 may 2025.
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Vol 12 No 1 (2014): JIFI
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