Isolation and Identification of Flavonoid Compounds in n-Buthanol Fraction of Dewa Leaves (Gynura pseudochina L.

  • RATNA DJAMIL UNIVERSITAS PANCASILA
  • CATHARINA YENNI UNIVERSITAS PANCASILA

Abstract

Plant of dewa leaves (Gynura pseudochina ( L. ) DC) from family Asteraceae is a plant that has been widely used for some treatments including blood circulation , trauma, bleeding and blood clot. In this research, the isolation and identification of flavonoid compounds in n-butanol fraction of the methanol extract of dewa leaves (Gynura pseudochina ( L. ) DC) has been carried out. The compound in Dewa leaves was isolated using maceration with methanol which was then partitioned by n-hexane solvent, ethyl acetate and n-butanol. The n-butanol phase was then isolated by paper chromatography with BAW (4:1:5) as a mobile phase which resulted in seven bands. Each of the bands was further identified using spectrophotometry UV-vis to show wavelength of maximum absorption that was given from only three bands for flavonoids namely NB-III, NB-V and NB-VI. Moreover, the bands were further eluated with 15% acetic acid as second mobile phase and the spot appeared in the presence of ammonia. The final isolates were identified by UV-vis spectrophotometer with addition of a shift reagent. The resulting spectrum from the isolates NB-III was considered as the one of flavonol compounds with the OH group at theposition of 3.7 and oxygenation at 6 or 8 and o– at OH group in ring B; NB-V isolates, OH groups at positions 3, 5, 7 and o– at OH group in ring B and NB-VI isolates, the OH group at position 5,7 and 8.

References

1. Sumaryono W. Teknologi pembuatan sediaan fitofarmaka skala industri. Warta Tumbuhan Obat Indonesia. Jakarta: 1996. 3(1):6.
2. Muhlisah F. Tanaman obat keluarga. Jakarta: Penebar Swadaya; 1998. 2, 17-9.
3. Winarto WP. Daun dewa budidaya dan pemanfaatan untuk obat. Jakarta: Penebar Swadaya; 2005. 3-9, 23-4.
4. Wijayakusuma H. Tanaman berkhasiat obat di Indonesia. Jilid I. Jakarta: Penerbit Pustaka Kartini; 1997. 42-3.
5. Dalimartha S. Atlas tumbuhan obat Indonesia. Jilid I . Jakarta: Trubus Agriwidya; 1999. 36-40.
6. Harborne JB. Metode fitokimia. Penuntun cara modern menganalisis tumbuhan. Edisi II. Diterjemahkan oleh Padmawinata K. Bandung: ITB; 1987. 6-21,71.
7. Markham KR. Cara mengidentifikasi flavonoid. Diterjemahkan oleh Padmawinata K. Bandung: ITB; 1988. 1-27, 38-47.
8. Farnsworth NR. Biological and phytochemical screening of plant. Journal of Pharmaceutical. 1996. 55(3).
9. Departemen Kesehatan Republik Indonesia. Parameter standar umum ekstrak tumbuhan obat. Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan; 2000. 10-2.
10. Wichtl M. Die Pharmakognostich Chemische Analyse. Frankfurt am Main: Akademische Verlagsgesselschaft; 1971. 153-5.
Published
2014-04-30
How to Cite
DJAMIL, RATNA; YENNI, CATHARINA. Isolation and Identification of Flavonoid Compounds in n-Buthanol Fraction of Dewa Leaves (Gynura pseudochina L.. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 12, n. 1, p. 93-98, apr. 2014. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/192>. Date accessed: 19 may 2024.
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Articles