Formulation of Tablets Containing Extracts of Soursop Leaves, Mangosteen Rind, Ling Zhi Mushroom and Its Antioxidant and Immunomodulator Activity Tests

  • SWASONO R. TAMAT UNIVERSITAS PANCASILA
  • ELIZA RAHMAN UHAMKA
  • LEONARDUS B.S. KARDONO PUSAT PENELITIAN KIMIA LIPI

Abstract

Investigation on formulation of film coated tablets containing combination of three active substances, i.e. extracts of soursop leaf, mangosteen rind and Ling Zhi mushroom has been carried out, which was expected to have high antioxidant and immunomodulator activities. The research consists of formulation of film coated tablets F1 containing extracts of soursop leaf-mangosteen rind-Ling Zhi mushroom (100:100:125 mg) and F2 (125:75:125 mg) in each 840 mg tablet. Full examination showed that film coated tablet F1 and F2 comply with the 4th Indonesian Pharmacopoeia, except for the dissolution test. Determination of antioxidant activity with DPPH method showed IC50 of F1 tablet was 215.93 μg/mL, IC50 of F2 tablet was 363.86 μg/mL, whereas IC50 of soursop leaf extract 602.64 μg/mL, IC50 of mangosteen rind extract 111.80 μg/mL and IC50 of Ling Zhi mushroom extract 2199.50 μg/mL. Determination of immunomodulatory activity by in vivo carbon clearance method showed a moderate phagocytic index of 1.385 for F1 tablet, 1.469 for F2 tablet, and <1 for Echinacea extract as positive control. Stability test performed based on the α-mangostin content showed that the shelf-life of F1 tablet was 1.865 years, and F2 tablet was 1.842 years. The result showed that film coated tablet F1 and F2 have both synergistic antioxidant and immunomodulatory activities as compared to their respective single extract. The result showed that the antioxidant activity and stability of film coated F1 tablet is better than F2 tablet, but the imunomodulatory activity of F2 tablet is better than F1 tablet.

References

1. Alamgir M, Uddin SJ. Recent advances on the ethnomedicinal plants as immunomodulatory agents. Journal Ethnomedicine, A Source of Complimentary Therapeutics. 2010. 227-44.
2. Vinardell MP, Mitjans M. Immunomodulatory effect of polyphenols: Electronic Journal of Environtment, Agricultural and Food Chemistry. 2008. 3357.
3. Jung HA, Su BN, Keller WJ, et al. Antioxidant xanthones from the pericarp of Garcinia mangostana (mangosteen). Journal of Cultural and Food Chemistry. 2006. (54): 2077-82.
4. Ahmat N, Nik Azmin NF, Abdul Ghani N, et al. Bioactive xanthones from the pericarp of Garcinia mangostana: Middle-East Journal of Scientific Research. 2010. 6 (2):123-7.
5. Yu L, Zhao M, Yang B, et al. Immunomodulatory and anticancer activities of phenolics from Garcia mangostana fruit pericarp. [Abstract]. Food Chemistry. 2009. 116(4):969-73. Diperoleh dari www.sciencedirect.com. Diakses pada tanggal 08 Desember 2011.
6. Akao Y, Nakagawa Y, Iinuma M, et al. Anticancer effects of xanthones from pericarp of mangosteen: Int. J. Mol. Sci. 2008. (9):355-70.
7. Moongkarndi P, Kosem N, Kaslungka S, et al. Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line. Journal of Ethnopharmacology. 2004. (90):161-6.
8. As’ari H. Pengaruh ekstrak kulit pericarp manggis (Garcinia mangostana) terhadap kadar kolesterol total, LDL dan HDL serum tikus putih jantan yang hiperkolesterolemia [tesis]. Surabaya: Airlangga University Library; 2010.
9. Chen SX, Wan M, Loh BN. Active constituents against HIV-1 protease from Garcinia mangostana [abstract]. Planta Med. 1996. 62(4):381-2.
10. Wasser SP. Reishi or Ling Zhi [Article]. Encyclopedia of Dietary Supplements. 2005. 603-19.
11. Tan BKH, Vanitha J. Immunomodulatory and antimicrobial effects of some traditional Chinese medicinal herbs: A review. Current Medicinal Chemistry. 2004. 11(11):1426.
12. Mardiana L, Ratnasari J. Ramuan & khasiat Sirsak: Cetakan I. Jakarta: Penebar Swadaya; 2011. 5-28.
13. Rudnic EM, Schwartz JB. Oral solid dosage forms, Remington’s the Science and Practice of Pharmacy. 21st Ed. Lippincott Williams and Wilkins; 1995. 889-917.
14. Lieberman HA, Lachman L. Pharmaceutical dosage forms: Tablets. Vol. 1. 2nd Ed. New York: Marcel Dekker; 1989. 109-431.
15. Aulton ME. Pharmaceutics the science of dosage form design. 2nd Ed. Edinburg: Churchill Livingstone; 2002. 133-5.
16. Yodhnu S, Sirikatitham A, Wattanapiromsakul C. Validation of LC for the determination of α-mangostin in mangosteen peel extract: A tool for quality assessment of Garcinia mangostana L. Journal of Chromatographic Science. March 2009. (47):185-9.
17. Pothirat W, Gritsanapan W. HPLC quantitative analysis method for the determination of α-mangostin in angosteen fruit rind extract. Thai J of Agric Sci. 2009. 42(1):7-12.
18. Kosem N, Han YH, Moongkarndi. Antioxidant and cytoprotective activities of methanolic extract from Garcinia mangostana hulls. Journal of the Science Society of Thailand. 2003. 33:283-92.
19. Juvekar AR, et al. In vitro and in vivo evaluation of immunomodulatory activity of methanol extract of Momordica charantia fruits. Drug Invention Today. 2009. 1(2):89-94.
20. Samir C, Patel et al. Scrutinizing the role of aqueous extract of Trapa bipinosa as an immunomodulator in experimental animals. International Journal Research Pharmacy Science. 2010. (1):13-9.
21. Departemen Kesehatan RI. Farmakope Indonesia edisi IV. Jakarta: Departemen Kesehatan Republik Indonesia; 1995.
22. United States Pharmacopeia. 34th Ed. Rockville: The United States Pharmacopeial Convention; 2011. 706, 742-3, 874-6.
23. British Pharmacopeia Comission. British Pharmacopoeia 2009: Vol. IV, London: British Pharmacopoeia Comission; 2008. Appendix XIIB A 303, XIIC A 313.
24. Purwaningsih S. Aktifitas antioksidan dan komposisi kimia keong mata merah (Cerithidae obtusa). Jurnal Ilmu Kelautan. 2012. 17(1):39-48. Diperoleh dari www.ejournal.undip.ac.id diakses tanggal 13 Januari 2013.
25. Wagner H. Immunomodulatory agents from plants. Basel: Birkhouse; 1999. 7.
26. WHO. Stability testing of active pharmaceutical ingredients and finished pharmaceutical products.
Annex 2, Appendix 1. World Health Organization Technical Report Series No. 953. 2009. 117-23.
27. Depkes RI. Keputusan Menteri Kesehatan Republik Indonesia No.701/MENKES/PER/VIII/2009 tentang Pangan Iradiasi. Jakarta: Depkes RI; 2009. 4.
28. Dewan Standarisasi Nasional-DSN. Cara uji cemaran mikroba. Jakarta: SNI 01-2897-1992.
29. Abdullah F. Cytotoxic and wild type P-53 promoting activities of selected Zingiberaceae species in colon cancer cell lines (tesis). Kuala Lumpur: Faculty of Science University of Malaya; 2009. 25-26.
30. Leswara ND. Radiofarmasi. Depok: Penerbit UI; 2005. 117, 119-20.
Published
2014-04-30
How to Cite
TAMAT, SWASONO R.; RAHMAN, ELIZA; KARDONO, LEONARDUS B.S.. Formulation of Tablets Containing Extracts of Soursop Leaves, Mangosteen Rind, Ling Zhi Mushroom and Its Antioxidant and Immunomodulator Activity Tests. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 12, n. 1, p. 124-138, apr. 2014. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/197>. Date accessed: 05 nov. 2024.
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