Gastroprotective Activity of Combination of Neem (Azadirachta indica A. Juss) Bark and Turmeric (Curucuma domestica Linn.) Rhizome Extracts on Rats Induced by Acetosal
Some pre-clinical and clinical studies reported that neem (Azadirachta indica A. Juss) bark and turmeric (Curcuma domestica Linn.) rhizome extracts demonstrated strong gastroprotective activities. In this studies, the effect of combination of neem bark and turmeric rhizome extracts were investigated on acetosal-induced gastric ulcer in rats.The combination is expected to produce synergic effect on the gastric ulcer. The combination of extracts was administered orally in various doses for 7 days prior to the ulcer induction. On the 8th-day rats were given asetosal 400 mg/kg bw to induce gastric ulcer. All rats were sacrificed 8 hours after asetosal induction for the determination of ulcer index, total acidity, mucus thickness and histophatological examination. The combination of neem bark extract (250 mg/kg bw) and turmeric rhizome extract (50 mg/kg bw) was significantly reduced the ulcer index (a = 0.05). This result was substantiated by histophatological examinations. Results of this study showed that combination of neem bark and turmeric rhizome extracts have synergic gastroprotective activities.
2. Rafatullah S, Tariq M, Al-Yahya MA, Mossa JS, Ageel AM. Evaluation of turmeric (Curcuma longa) for gastric and duodenal antiulcer activity in rats. J Ethnopharm. 1990.29(1):25-34.
3. Maheshwari RK, Singh AK, Gaddipati J, Srimal RC. Multiple biological activities of curcumin: A short review. Life Sci. 2006.78:2081-7.
4. Prucksunand C, Indrasukhsri B, Leethochawalit M, Hungsprreugs K. Phase II clinical trial on effect of the long tumeric (Curcuma longa Linn) on healing of peptic ulcer. Southeast Asian J Tropical Med Public Health. 2001.32(1):208-15.
5. Jayaprakasha GK, Rao JMRL, Sakariah KK. Chemistry and biological activities of Curcuma longa. Trends Food Sci Tech. 2005.16:533-48.
6. E/S/C/O/P Monograph The Scientific Foundation for Herbal Medicinal Product. 2nd ed. Thieme, United Kingdom: Thieme; 2001. 107-16.
7. Bandyopadhyay U, Biswas K, Chattophadyay I, Banerjee RK. Process for isolation of an active principle from Azadirachta indica useful for controlling gastric hyperacidity and gastric ulceration. US Patent. 1998: 5,730,986.
8. Bandyopadhyay U, Biswas K, Chatterjee R, Bandyopadhyay D, Chattopadhyay I, Kumar Ganguly C, et al. Gastroprotective effect of neem (Azadirachta indica) bark extract: Possible involment of H+-K+-ATPase inhibition and scavenging of hydroxyl radical. Life Sci. 2002.71:2845-65.
9. Bandyopadhyay U, Biswas K, Segupta A, Moitra P, Dutta P, Sarkar D, et al. Clinical studies on the effect of neem (Azadirachta indica) bark extract on gastric secretion and gastroduodenal ulcer. Life Sci. 2004.75(23):2867-78.
10. Raji Y, Ogunwande IA, Osadebe CA, John G. Effect of Azadirachta indica extract on gastric ulceration and acid secretion in rats. J Ethnopharm. 2004.90:167-70.
11. Parmar NS, Prakash S. Screening methods in pharmacology. Oxford: Alpha Science International Ltd; 2006. 265-6.
12. Stevens A, James S Lowe, Barbara Young. Basic histophatology. 4th ed. Philadelphia: Churchill Livingstone; 2002. 138-142.
13. WHO Monograph on Selected medicinal plants. 1999. 1. 117-24.
14. Mills S and Bone K. Principles and practice of Phytotherapy: Modern herbal Medicine. New York: Churchill Livingstone; 2000. 569-80.
15. Brzozowski T, Konturek PC, Konturek SJ, Brzozowska I, Pawlik T. Role of prostaglandins in gastroprotection and gastric adaptation. J Physiol Pharm. 2005.56(5):33-55.
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