Phagocytic Macrophage Activity of Fractions from Methanolic Leaf Extract of Red Betel (Piper crocatum Ruiz & Pav.) In Vitro

  • YUSTINA SRI HARTINI UNIVERSITAS SANATA DHARMA
  • SUBAGUS WAHYUONO UNIVERSITAS GADJAH MADA
  • SITARINA WIDYARINI UNIVERSITAS GADJAH MADA
  • AGUSTINUS YUSWANTO UNIVERSITAS GADJAH MADA

Abstract

Plants can be sources of immunomodulatory agents. Piper crocatum Ruiz & Pav. (red betel) leaves have been used to heal many diseases, especially to increase endurance. The aim of this research was to investigate the imunomodulatory effect of methanolic leaf extracts of Piper crocatum Ruiz & Pav. and its fractions by in vitro phagocytic macrophage activity test. The result showed that methanolic extract of Piper crocatum Ruiz & Pav. 75μg/mL could stimulate phagocytic macrophage activity equal to 100μg/mL product-X® that contained Echinacea extract. Separation of the extract by vacuum liquid chromatography using gradient solvent n-hexane - ethyl acetate yielded 5 fractions. The second fraction showed the highest activity. It contained alkaloid and terpenoid, while the extract also contained flavonoid and essential oil. Alkaloid and/or terpenoid could be the compound in the leaves of Piper crocatum Ruiz & Pav. that responsible increasing phagocytic macrophage activity.

References

1. Wagner H, Kraus S, Jurcic K. Search for potent immunostimulating agents from plants and other natural sources, In: Wagner H, editor. Immunomodulatory agents from plants. Basel, Switzerland: Birkhauser Verlag; 1999. 1-40.
2. Wagner H, Kraus S, Jurcic K. . Chemistry, analysis and immunological investigations of Echinachea phytopharmaceuticals. In: Wagner H, editor. Immunomodulatory agents from plants. Basel, Switzerland: Birkhauser Verlag; 1999. 41-89.
3. Alamgir M and Uddin SK. Recent advances on the ethnomedicinal plants as immunomodulatory agents. Ethnomedicine. 2010. 3:227-44.
4. Kumar S, Gupta P, Sharma S, Kumar D. A review on immunostimulatory plants. Journal of Chinese Integrative Medicine. 2011. 9:1-18.
5. Kanjwani DG, Marathe TP, Chipunkar SV, Fan Sathaye SS. Evaluation of immunomodulatory activity of metanolic extract of Piper betel. Scandinavian Journal of Immunology. 2008. 67: 589-93.
6. Sunila ES and Kuttan G. Immunomodulatory and antitumor activity of Piper longum Linn. and piperine. Journal of Ethnopharmacology. 2004. 90:339-46.
7. Parmar VS, Jain SC, Bisht KS, Jain R, Taneja P, Jha A, et al. Phytochemistry of genus Piper. Phytochemistry. 1997. 46:597-673.
8. Manoi F. Sirih Merah sebagai tanaman obat multi fungsi. Warta Pusat Penelitian dan Pengembangan Perkebunan. 2007. 13:2-6.
9. Wicaksono BD, Handoko YA, Arung ET, Kusuma IW, Yulia D, Pancaputra AN, et al. Antiproliferative effect
0.005.0010.0015.0020.0025.0030.0035.00presentase
of metanol extract of Piper crocatum Ruiz & Pav leaves on human breast (T47D) cells in vitro. Tropical Journal of Pharmaceutical Research. 2009. 8:345-52.
10. Suratmo. Aktivitas antioksidan dan antikanker ekstrak daun Sirih Merah (Piper crocatum) [tesis]. Yogyakarta: Prodi Ilmu Kimia Program Pascasarjana Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Gadjah Mada; 2008.
11. Ponkshe CA and Indap MM. In vivo and in vitro evaluation for immunomodulatory activity of three marine animal extracts with reference to phagocytosis. Indian Journal of Experimental Biology. 2002. 40:1399-400.
12. Coll JC and Bowden BF. The application of vacuum liquid chromatography to the separation of terpene mixtures. J Nat Prod. 1986. 49:934-36.
13. Leijh PCJ, Furth RV, Van Zwet TL. In vitro determination of phagocytosis and intracellular killing by polymorphonuclear and mononuclear phagocytes. In: Weir DM, editor. Cellular immnunology. 4th Ed. Oxford: Blackwell Scientific Publications; 1986.
14. Wijanarko H. Aktivitas phalerin hasil isolasi dari daun Mahkota Dewa (Phaleria macrocarpa (Scheff) Boerl) sebagai pemacu fagositosis makrofag dan antiradikal in vitro [tesis]. Yogyakarta: Sekolah Pasca Sarjana, Universitas Gadjah Mada; 2005.
15. Wahyuniari IAI. Pengaruh pemberian minyak Buah Merah (Pandanus conoideus Lamp) pada respon imun seluler setelah infeksi Listeria monocytogenes [tesis]. Yogyakarta: Program Studi Ilmu Kedokteran Dasar dan Biomedis, Sekolah Pascasarjana Universitas Gadjah Mada; 2006.
16. Derre I, Isberg RR. Macrophages from mice with the restrictive lgn1 allele exhibit multifactorial resistance to Legionella pneumophila. Infect. Immun. 2004. 72(11):6221-9.
17. Ciulei J. Metodology for analysis of vegetables and drugs. Bucharest Rumania: Faculty of Pharmacy; 1981. 11-26.
18. Wagner H, Bladt S, Zgainski EM. Plant drug analysis, A thin layer chromatography atlas. Berlin Heidelberg: Springer; 1984. 346-9, 42-4.
19. Samuelsson G. Drugs of natural origin. 4th Ed. Sweden: Swedish Pharmaceutical Press; 1999.
20. Sutikno, Nugroho LH, Yuliati IR, Priyono Y. Anatomi dan profil minyak atsiri akar, batang dan daun sepuluh spesies genus piper koleksi Kebun Raya Bogor. [laporan Penelitian]. Yogyakarta: Fakultas Biologi Universitas Gadjah Mada; 2011.
21. Sanchez S, Paredes SD, Sanchez CL, Barriga C, Reiter RJ, Rodriquez AB. Tryptophan administration in rats enhances phagocytic function and reduces oxidative metabolism. Neuro Endocrinol Lett. 2008. 29(6):1026-32.
Published
2013-09-30
How to Cite
HARTINI, YUSTINA SRI et al. Phagocytic Macrophage Activity of Fractions from Methanolic Leaf Extract of Red Betel (Piper crocatum Ruiz & Pav.) In Vitro. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 11, n. 2, p. 108-115, sep. 2013. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/202>. Date accessed: 31 may 2020.
Section
Articles