Phagocytic Macrophage Activity of Fractions from Methanolic Leaf Extract of Red Betel (Piper crocatum Ruiz & Pav.) In Vitro

  • YUSTINA SRI HARTINI UNIVERSITAS SANATA DHARMA
  • SUBAGUS WAHYUONO UNIVERSITAS GADJAH MADA
  • SITARINA WIDYARINI UNIVERSITAS GADJAH MADA
  • AGUSTINUS YUSWANTO UNIVERSITAS GADJAH MADA

Abstract

Plants can be sources of immunomodulatory agents. Piper crocatum Ruiz & Pav. (red betel) leaves have been used to heal many diseases, especially to increase endurance. The aim of this research was to investigate the imunomodulatory effect of methanolic leaf extracts of Piper crocatum Ruiz & Pav. and its fractions by in vitro phagocytic macrophage activity test. The result showed that methanolic extract of Piper crocatum Ruiz & Pav. 75μg/mL could stimulate phagocytic macrophage activity equal to 100μg/mL product-X® that contained Echinacea extract. Separation of the extract by vacuum liquid chromatography using gradient solvent n-hexane - ethyl acetate yielded 5 fractions. The second fraction showed the highest activity. It contained alkaloid and terpenoid, while the extract also contained flavonoid and essential oil. Alkaloid and/or terpenoid could be the compound in the leaves of Piper crocatum Ruiz & Pav. that responsible increasing phagocytic macrophage activity.

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Published
2013-09-30
How to Cite
HARTINI, YUSTINA SRI et al. Phagocytic Macrophage Activity of Fractions from Methanolic Leaf Extract of Red Betel (Piper crocatum Ruiz & Pav.) In Vitro. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 11, n. 2, p. 108-115, sep. 2013. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/202>. Date accessed: 27 july 2024.
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Vol 11 No 2 (2013): JIFI
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