Response of Leuconostoc mesenteroides and Weissella confusa strains on pH of Growth Condition using Antibiotics as Indicator
Abstract
Lactic acid bacteria is known to contribute to human health as exopolysaccharide producer, bacteriocin producer, live vaccins, starter in fermentation process and as probiotics. This study aimed to observe Leuconostoc mesenteroides and Weisella confusa strains response under manipulated growth condition for carbon source composition and on pH as a model, using various antibiotics as indicator. MRS and modified MRS (50% normal dextrose concentration) were used. Modification of pH representing acidic and alkaline condition were 4.6 and 9.0. Disc diffusion method was employed using six different antibiotics. Result showed that all strains grown in acidic standard MRS tend to be more sensitive to amoxicillin (Am), chloramphenicol (Ch) and polymyxin B (PoB), but more resistant to ciprofloxacin (Ci), while in the acidic modified MRS, the strains respond more sensitive to Am, Ch, Ci and PoB. When grown in alkaline standard MRS, all strains respond more sensitive to Am, Ci and PoB, but more resistant to Ch, while in alkaline modified MRS, they respond more resistant to Am and Ch but more sensitive to Ci and PoB. Most of the strains did not show inhibition against vancomycin and none of strains showed inhibition against sulphamethoxazole-trimethoprim under conditions applied.
References
2. Mathur S, Singh R. Antibiotic resistance in food lactic acid bacteria–A review. International Journal of Food Microbiology. 2005.105:281-95.
3. Van de Guchte M, Serror P, Chervaux C, Smokvina T, Ehrlich S, Maguin E. Stress responses in lactic acid bacteria. Antonie van Leeuwenhoek. 2002. 82: 187-216.
4. Malik A, Felicia, Radji M, Oetari A. Identifikasi bakteri asam laktat penghasil eksopolisakarida asal sumber lokal menggunakan gen penyandi 16S rRNA. Sains Indonesia. 2007.12:1-6.
5. Malik A, Radji M, Kralj S, Dijkhuizen L. Screening of lactic acid bacteria from Indonesia reveals glucansucrase and fructansucrase genes in two different Weissella confusa strains from soya. FEMS Microbiol Lett; 2009. 300:131-8.
6. Malik A, Hermawati AK, Hestiningtyas M, Soemiati A, Radji M. Isolasi dan skrining molekuler bakteri asam laktat pembawa gen glukansukrase dari makanan dan minuman mengandung gula. Makara (Sains). 2010.14:57-62.
7. De Man JC, Rogosa M, Sharpe ME. A medium for the cultivation of Lactobacilli. J Appl Bacteriol.1960. 23:130-5.
8. Trihendradi C. Step by step SPSS 16 analisis data statistik. Yogyakarta: Penerbit Andi; 2009.
9. Hutkins RW, Nannen NL. pH homeostasis in lactic acid bacteria. Journal of Dairy Sciences. 1993.76: 2354-65.
10. Bauman RW. Microbiology. San Fransisco: Pearson Benjamin Cummings; 2004. 168, 174, 188-9.
11. Vela AI, Porrero C, Goyache J, Nieto A, Sanchez B, Briones V, Moreno MA, Dominguez L, Fernandez-Garayzabal JF. Weissella confusa infection in primate (Cercopithecus mona). Emerging Infectious Diseases. 2003.9:1307-9.
12. Handwerger S, Pucci M, Volk K, Liu J, Lee M. Vancomycin-resistant Leuconostoc mesenteroides and Lactobacillus casei synthesize cytoplasmic peptidoglycan precursors that terminate in lactate. Journal of Bacteriology. 1994.176:260-4.
13. Ammor MS, Florez AB, Mayo B. Antibiotic resistance in non-Enterococcal lactic acid bacteria and Bifidobacteria. Food Microbiology. 2007.24: 559-70.
14. Setiabudy R. Golongan kuinolon dan fluorokuinolon. In: Gunawan SG, Setiabudy R, editors. Farmakologi dan Terapi. Edisi 5. Jakarta: Departemen Farmakologi dan Terapeutik; 2007. 718-22.
15. Sawatari Y, Yokota A. Diversity and mechanisms of alkali tolerance in Lactobacilli. Applied and Environmental Microbiology; 2007.73:3909-15.
16. Yoshiok S, Stella VJ. Stability of drugs and dosage forms. New York: Kluwer Academic; 2002.
Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.
This licence applies to Author(s) and Public Reader means that the users mays :
- SHARE:
copy and redistribute the article in any medium or format - ADAPT:
remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication) - ALIKE:
If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original. - NO ADDITIONAL RESTRICTIONS:
You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
It does however mean that when you use it you must:
- ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
You may not:
- NONCOMMERCIAL: You may not use the article for commercial purposes.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.