Response of Leuconostoc mesenteroides and Weissella confusa strains on pH of Growth Condition using Antibiotics as Indicator

  • AMARILA MALIK UNIVERSITAS INDONESIA
  • DEWI PUSPITA UNIVERSITAS INDONESIA
  • RIZKI AYU AMALIA UNIVERSITAS INDONESIA

Abstract

Lactic acid bacteria is known to contribute to human health as exopolysaccharide producer, bacteriocin producer, live vaccins, starter in fermentation process and as probiotics. This study aimed to observe Leuconostoc mesenteroides and Weisella confusa strains response under manipulated growth condition for carbon source composition and on pH as a model, using various antibiotics as indicator. MRS and modified MRS (50% normal dextrose concentration) were used. Modification of pH representing acidic and alkaline condition were 4.6 and 9.0. Disc diffusion method was employed using six different antibiotics. Result showed that all strains grown in acidic standard MRS tend to be more sensitive to amoxicillin (Am), chloramphenicol (Ch) and polymyxin B (PoB), but more resistant to ciprofloxacin (Ci), while in the acidic modified MRS, the strains respond more sensitive to Am, Ch, Ci and PoB. When grown in alkaline standard MRS, all strains respond more sensitive to Am, Ci and PoB, but more resistant to Ch, while in alkaline modified MRS, they respond more resistant to Am and Ch but more sensitive to Ci and PoB. Most of the strains did not show inhibition against vancomycin and none of strains showed inhibition against sulphamethoxazole-trimethoprim under conditions applied.

References

1. Soomro AH, Masud T, Anwar K. Role of lactic acid bacteria (LAB) in food preservation and human health–A review. Pakistan Journal of Nutrition. 2002: 20-42.
2. Mathur S, Singh R. Antibiotic resistance in food lactic acid bacteria–A review. International Journal of Food Microbiology. 2005.105:281-95.
3. Van de Guchte M, Serror P, Chervaux C, Smokvina T, Ehrlich S, Maguin E. Stress responses in lactic acid bacteria. Antonie van Leeuwenhoek. 2002. 82: 187-216.
4. Malik A, Felicia, Radji M, Oetari A. Identifikasi bakteri asam laktat penghasil eksopolisakarida asal sumber lokal menggunakan gen penyandi 16S rRNA. Sains Indonesia. 2007.12:1-6.
5. Malik A, Radji M, Kralj S, Dijkhuizen L. Screening of lactic acid bacteria from Indonesia reveals glucansucrase and fructansucrase genes in two different Weissella confusa strains from soya. FEMS Microbiol Lett; 2009. 300:131-8.
6. Malik A, Hermawati AK, Hestiningtyas M, Soemiati A, Radji M. Isolasi dan skrining molekuler bakteri asam laktat pembawa gen glukansukrase dari makanan dan minuman mengandung gula. Makara (Sains). 2010.14:57-62.
7. De Man JC, Rogosa M, Sharpe ME. A medium for the cultivation of Lactobacilli. J Appl Bacteriol.1960. 23:130-5.
8. Trihendradi C. Step by step SPSS 16 analisis data statistik. Yogyakarta: Penerbit Andi; 2009.
9. Hutkins RW, Nannen NL. pH homeostasis in lactic acid bacteria. Journal of Dairy Sciences. 1993.76: 2354-65.
10. Bauman RW. Microbiology. San Fransisco: Pearson Benjamin Cummings; 2004. 168, 174, 188-9.
11. Vela AI, Porrero C, Goyache J, Nieto A, Sanchez B, Briones V, Moreno MA, Dominguez L, Fernandez-Garayzabal JF. Weissella confusa infection in primate (Cercopithecus mona). Emerging Infectious Diseases. 2003.9:1307-9.
12. Handwerger S, Pucci M, Volk K, Liu J, Lee M. Vancomycin-resistant Leuconostoc mesenteroides and Lactobacillus casei synthesize cytoplasmic peptidoglycan precursors that terminate in lactate. Journal of Bacteriology. 1994.176:260-4.
13. Ammor MS, Florez AB, Mayo B. Antibiotic resistance in non-Enterococcal lactic acid bacteria and Bifidobacteria. Food Microbiology. 2007.24: 559-70.
14. Setiabudy R. Golongan kuinolon dan fluorokuinolon. In: Gunawan SG, Setiabudy R, editors. Farmakologi dan Terapi. Edisi 5. Jakarta: Departemen Farmakologi dan Terapeutik; 2007. 718-22.
15. Sawatari Y, Yokota A. Diversity and mechanisms of alkali tolerance in Lactobacilli. Applied and Environmental Microbiology; 2007.73:3909-15.
16. Yoshiok S, Stella VJ. Stability of drugs and dosage forms. New York: Kluwer Academic; 2002.
Published
2013-09-30
How to Cite
MALIK, AMARILA; PUSPITA, DEWI; AMALIA, RIZKI AYU. Response of Leuconostoc mesenteroides and Weissella confusa strains on pH of Growth Condition using Antibiotics as Indicator. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 11, n. 2, p. 127-133, sep. 2013. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/206>. Date accessed: 27 apr. 2024.
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Vol 11 No 2 (2013): JIFI
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