Combination of Morinda citrifolia L. Fruits and Carica papaya L. Leaves Ethanolic Extract as Hepatoprotective during Tuberculosis Treatment

  • TITIK SUNARNI UNIVERSITAS SETIA BUDI
  • RINI PRASTIWI UNIVERSITAS SETIA BUDI
  • MARDIYONO MARDIYONO UNIVERSITAS SETIA BUDI
  • YUDI RINANTO UNIVERSITAS SEBELAS MARET

Abstract

Hepatoprotective activity of Morinda citrifolia L. fruits and Carica papaya L. leaves ethanolic extract have been investigated in antituberculosis-induced rats. Forty rats were divided into following eight groups: normal control group was treated with normal feed and water; negative control group was treated with isoniazid and rifampicin, positive control group was treated with isoniazid, rifampicin and Methicol®, and the last five groups were treated with isoniazid, rifampicin and combination of M. citrifolia L. fruits and C. papaya L. leaves extract on various doses. All groups were treated daily for 27 days. Serum levels of bilirubin, alanine aminotransferase (ALT) as well as aspartate aminotransferase (AST) were determined on day -0, 14, 21 and 28 to monitor the liver function. All rats were sacrified on day-28 for liver histology observation. Combination of M. citrifolia extract 20 mg/200 g bw and C. papaya extract 120 mg/200 g bw significantly decreased the serum level of ALT, AST as well as bilirubin. The histology study showed that this combination could prevent the liver damaged based on the lower percentage of necrosis (27.83%), as compared to that of negative control (47.47%).

References

1. Arsyad Z. Evaluasi faal hati pada penderita tuberkulosis paru yang mendapat terapi obat anti tuberkulosis. Cermin Dunia Kedokteran. 1996. 110:15-8.
2. Adhvaryu MR, Reddy NM, Vakharia BC. Prevention of hepatotoxicity due to anti tuberculosis treatment: A novel integrative approach. World J Gastroenterol. 2008.14(30):4753-62.
3. Adhvaryu MR, Reddy NM, Parabia MH. Effects of four Indian medicinal herbs on isoniazid, rifampicin and pyrazinamide induced hepatic injury and immunosuppression in guinea pigs. India World J Gastroenterol. 2007.13(23):3199-205.
4. Hembing W. Tumpas hepatitis dengan ramuan herbal. Jakarta: PT Elex Media Komputindo Gramedia; 2008. 34-42.
5. Ruwit Y. Pengaruh ekstrak etanol 70% daun pepaya (Carica papaya L.) terhadap kadar bilirubin serum tikus putih yang diinduksi isoniazid dan rifampisin [skripsi]. Surakarta: Fakultas Farmasi Universitas Setia Budi; 2009.
6. Hariana A. Tumbuhan obat dan khasiatnya. Jakarta: Penerbit Swadaya; 2008. 118-24.
7. Marsono H. Pengaruh praperlakuan ekstrak etanolik buah mengkudu (Morinda citrifolia L.) terhadap aktivitas enzim GPT dan GOT akibat pemberian paracetamol pada tikus putih jantan galur Wistar [skripsi]. Surakarta: Fakultas Farmasi Universitas Setia Budi; 2007.
8. Zain A. Pengaruh pemberian perasan buah mengkudu (Morinda citrifolia L.) terhadap gambaran histopatologi ginjal tikus putih (Rattus norvegicus) diet lemak tinggi [skripsi]. Surakarta: Fakultas Farmasi Universitas Setia Budi; 2006.
9. Irfianti TR. Pengaruh pemberian mengkudu (Morinda citrifolia L.) terhadap hepatotoksisitas karbon tetraklorida pada mencit [skripsi]. Surakarta: Fakultas Kedokteran Universitas Sebelas Maret; 2007.
10. Rendi I. Pengaruh ekstrak etanol 70% buah mengkudu (Morinda citrifolia L.) terhadap aktivitas enzim ALT dan AST pada tikus putih galur wistar yang diinduksi isoniazid dan rifampisin [skripsi]. Surakarta: Fakultas Farmasi Universitas Setia Budi; 2009.
11. Ridwan DK. Pengaruh ekstrak etanol 70% buah mengkudu (Morinda citrifolia L.) terhadap kadar bilirubin serum pada tikus yang diinduksi isoniazid dan rifampisin [skripsi]. Surakarta: Fakultas Farmasi Universitas Setia Budi; 2009.
12. Ronny AW. Efek ekstrak etanol 70% buah mengkudu (Morinda citrifolia L.) terhadap histologi hati tikus putih yang diinduksi dengan isoniazid dan rifampisin [skripsi]. Surakarta: Fakultas Farmasi Universitas Setia Budi; 2009.
13. Depkes RI. Materia Medika Indonesia. Jilid V. Jakarta; 1989. 119.
14. Robinson T. Kandungan organik tumbuhan tinggi diterjemahkan oleh Kosasih Padmawinata. Bandung: Penerbit ITB; 1995. 191-3.
15. Departemen Kesehatan dan Kesejahteraan Sosial RI. Inventaris tanaman obat Indonesia. Jilid 1. Jakarta: Depkes-Sos RI; 2000. 51-52.
16. Harborne JB. Metode fitokimia: penuntun cara modern menganalisis tumbuhan diiterjemahkan oleh Kosasih Padmawinata dan Iwang Soediro. Bandung: Penerbit ITB; 1987. 234-245.
17. Pal R, Vaiphei K, Sikander A, Singh K, Rana SV. Effect of garlic on isoniazid and rifampisin-induced hepatic injury in rats. India World J Gastroenterology. 12(4):636-9.
18. Asiamaya. Buah mengkudu. 2004. diambil dari: http://www.Asiamaya.com/jamu/isi/ mengkudu_Morinda citrifolia, html, diakses 29 Juni 2009.
Published
2013-09-30
How to Cite
SUNARNI, TITIK et al. Combination of Morinda citrifolia L. Fruits and Carica papaya L. Leaves Ethanolic Extract as Hepatoprotective during Tuberculosis Treatment. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 11, n. 2, p. 160-166, sep. 2013. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/211>. Date accessed: 05 nov. 2024.
Section
Articles