Activity of Fenugreek Seed (Trigonella foenum-graecum L.) on Proliferation and Apoptotic Potency of MCF-7 Breast Cancer Cells

  • KURNIA AGUSTINI UNIVERSITAS INDONESIA
  • FRANS SUYATNA UNIVERSITAS INDONESIA
  • NURJATI CHAIRANI SIREGAR UNIVERSITAS INDONESIA
  • WAHONO SUMARYONO BADAN PENGKAJIAN DAN PENERAPAN TEKNOLOGI

Abstract

Fenugreek seed (Trigonella foenum-graecum L.) is one of Indonesian medicinal plant with many known activities such as antidiabetic, antihypercholesterolemia, phytoestrogen and anticancer. Fenugreek seed contains steroidal sapogenines (i.e. diosgenin, yamogenin, gitogenin, tigogenin and trigoneoside), alkaloid trigonellin and flavonoid (i.e vitexin, orientin). This experiment was carried out to investigate the cytotoxic activity and its potency to induce apoptotic on breast cancer cell line, MCF-7. Cytotoxic activity was carried out by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) method. Fenugreek seed was prepared by extraction (methanolic and ethanolic extract) and fractionation using solvents with different polarity (hexane, ethylacetic, buthanolic and water fractions). The potency of apoptotic induction assay was carried out using flowcytometric method staining with propidium iodide (20μg/mL) on 100 ppm sample. Apoptotic potency was evaluated based on % population of cells in subG0/G1 phase. Results showed that ethylacetic fraction has the lowest IC50 (41.81 ppm) and significantly (p<0.05) increase % population of cells in subG0/G1 phase (20.87%) on 100 ppm concentration. It is concluded that fenugreek seed posses cytotoxic activity and potency of apoptotic induction on MCF-7 cells.

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Published
2013-04-30
How to Cite
AGUSTINI, KURNIA et al. Activity of Fenugreek Seed (Trigonella foenum-graecum L.) on Proliferation and Apoptotic Potency of MCF-7 Breast Cancer Cells. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 11, n. 1, p. 13-20, apr. 2013. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/226>. Date accessed: 05 feb. 2025.
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Vol 11 No 1 (2013): JIFI
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