Evaluation of Diuretic Activity of Ethanolic Extract from Fruits of Belimbing Wuluh (Averrhoa bilimbi) as a Natural Diureticum: Urine Sodium and Potassium Concentrations and pH

  • ANDRIYANTO ANDRIYANTO INSTITUT PERTANIAN BOGOR
  • PONIMAN PONIMAN INSTITUT PERTANIAN BOGOR
  • ABADI SUTISNA INSTITUT PERTANIAN BOGOR
  • WASMEN MANALU INSTITUT PERTANIAN BOGOR

Abstract

Previous study reported that Averrhoa bilimbi fruits had a potency as a natural diuretic. This research was conducted to examine the effects of ethanolic extract of Averrhoa bilimbi fruits on diuretic activity, urine sodium and potassium concentrations, and pH of urine male rats Sparague-Dawley. Fifteen rats were divided into 5 groups and 3 replications. Group 1 was given saline (negative control), group 2 was given furosemide 20 mg/kg bw (positive control), group 3 was given extract of Averrhoa bilimbi 0.44 g/kg bw, group 4 was given extract of Averrhoa bilimbi 0.88 g/kg bw, group 5 was given extract of Averhoa bilimbi 1.75 g/kg bw. Averrhoa bilimbi extract, saline, and furosemide were administred orally. The diuretic activity was determined by measuring cumulative urine volume for 10 hours post administration. The result showed that the extract of Averrhoa bilimbi at dosages of 0.88 and 1.75 g/kg bw increased sodium and potassium excretion by 18-36% and 22-27%, respectively. On the other hand, the increased diuretic activity and sodium and potassium excretion dramatically decreased urine pH. It was concluded that extract of Averrhoa bilimbi at dosages of 0.88 and 1.75 g/kg bw increased diuretic activity with enchanced sodium and potassium excretion in urine. The extract of Averrhoa bilimbi cosisted of alkaloid, flavonoid, and saporin that were assumed to have synergic activities as natural diuretics.

References

1. Johari M. Rumah Sakit Jantung di Semarang. 2003. diambil dari http://eprints.undip.ac.id/7368. diakses 24 Juni, 2011.
2. Felker GM. How to use diuretics in heart failur. 2009. diambil dari http://www.springerlink.com/content/c)m6762061255070/. diakses 18 Juni, 2011.
3. Asare K. Management of loop diuretic resistance in the intensive care unit. American Journal Health-Sys Pharmacology. 2009. 66(15):1635-40.
4. Douglas E, Barbieri, Fernando F, Filho R, Ribeiro AB, Zanella MT. Diuretic-induced potassium depletion and glucose intolerance are not related to hyperactivity of the renin-angiotensin-aldosterone system in hypertensive patients with the metabolic syndrome. The Journal of Clinicsl Hypertention. 2009. 11(10):549-54.
5. Vormfelde SV, Sehrt D, Toliat MR, Schirmer M, Meineke I, Tzvetkov M, et al. Genetic variation in the renal sodium transporters nkcc2, ncc, and enac in relation to the effects of loop diuretic drugs. Nature. 2007. 82(3):300-9.
6. Felker MG. Diuretics management in hearth failur [Supplement 1]. Review Paper. 2010. 68-72.
7. Brater DC. Diuretic therapy. The New England Journl of Medicine. 1998. 339(6):387-95.
8. Katzung BG. Farmakologi dasar dan klinik. Ed. I. Jakarta: Penerbit Salemba Medika; 2001. 1-100.
9. Englert J, Harnischfeger G. Diuretics action aqueous orthosiphon extrac in rats. Planta Medica. 1992. 58:237-8.
10. Bipat R, Tolsie JR, Joemnanbaks RF, Gummels JM, Klavermeide J, Jhanjan N, et al. Effects of plants populary used againts hypertension on nornephineprinestimulated guinea pig atria. Pharmacognosy. 2008. 4(13):12-9.
11. Fuchs FD, Fuchs SC, Moreira LB, Gus M, Nóbrega AC, Poli-de-Figueiredo CE, et al. Prevention of hypertension in patients with pre-hypertension: protocol. Trials Journal.2011. 12:65-72.
12. Paripovic D, Kostic M, Spasojevic B, Kruscic D, Peco-Antic A. Masked hypertension and hidden uncontrolled hypertension after renal transplantation. Pediatr Nephrol. 2010. 25:1719-24.
13. Ardeleanu E, Popa I, Gruici A, Bruca E, Puricel I, Râmneantu A, et al. Incidence, risk factors and complications of white coat hypertension in family medicine practice. Fiziologia - Physiology. 2011. 21(69):26-9.
14. Manimunda SP, Sugunan AP, Benegar V, Balakrishna N, Rao MV, Pésala KS. Association of hypertension with risk factors & hypertension related behaviour among the aboriginal Nicobarese tribe living in Car Nicobar Island, India. Indian Journal Medicine Res. 2011. 13: 287-93.
15. Doumas M, Papademetriou V, Douma S, Faselis C, Tsioufis K, Gkaliagkousi E, et al. Review Article: Benefits from treatmennt and control of patients with resistant hypertension. International Journal of Hypertension. 2011.2011:1-8.
16. Stam J, Risbano MG, Mathier MA. Overview of current therapeutic approaches for pulmonary hypertension. Pulmonary Circulation. 2011. 1(2):138-59.
17. Angeli P. Combined versus sequential diuretic treatment of ascites in non-azotaemic patients with cirrhosis: result of an open randomised clinical trial [abstract]. 2009. diambil dari http://gut.bmj.com/content/59/01/98. diakses 18 Juni, 2011.
18. Agunu A, Abdyrahman EM, Andrew GO, Muhhammed Z. Diuretic activity of the stem-bark extracts of steganotaenia araliaceahoehst. Journal of Ethnopharmacology. 2005.96:471-5.
19. Grikiniene J, Stakisaitis D, Tschaika M. Acute influence of sodium valproate on sodium and chloride urinary excretion in rats. Acta Medica Lituanica. 2005. 12(2):45-50.
20. Pushpuraj PN, Tan BKH, Tan CH. The mechanism of hypoglycemic of the semi-purified fractions ofAverrhoa bilimbi in streptozotocin-diabetic rats. Life Sciences. 2001. 70:535-47.
21. Hernani, Winarti C, Marwati T. Pengaruh pemberian ekstrak daun belimbing wuluh terhadap penurunan tekanan darah pada hewan uji. Jurnal Pascapanen. 2009. 6(1):54-61.
22. Andriyanto, Kusumorini N, Yuskha F. Potensi ektrak etanol buah belimbing wuluh (Averrhoa bilimbi L) sebagai alternatif sediaan diuretik alami. Jurnal Ilmu Kefarmasian Indonesia. 2011. 9(2):78-84.
23. Shenoy JP, Pai PG, Shoeb A, Gokul P, Kulkarni A, Kotian MS. An evaluation of diuretic activity of Morinda citrifolia (Linn.) (Noni.) fruit juice in normal rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2011. 3(2):119-21.
24. Mamun MM, Billah MM, Aschek MA, Ahasan MM, Hossain MJ, Sultana T. Evaluation of diuretic activity of Ipomea quatica (kalmisak) in Mice Model Study. Journal Medical Sciences. 2003. 3(5-6):395-400.
25. Mahmod H, Bachar SC, Saiful M, Ali MS. Analgesic and diuretic activity of Curcuma xantorrhiza. J. Pharmaceutical Sciences.2004. 3:1-12.
26. Szentmihlyi K, Kery A, Then M. Potassium sodium ratio for the characterization of medicinal plant extracts with diuretic activity. Phytother Res. 1998. 12:163-6.
27. Hook I, McGee A, Henman M. Evaluation of dandelin for diuretic activity and variation in potassium content. Pharmaceutical Biology.1993. 31(1):29-34.
28. Herrera M, Abdala S, Benjumea D, Perez-Paz P. Diuretic activity of withania aristata: an endemic canary island species. Journal Ethnopharmacology. 20017. 11(3):487-91.
29. Mary JM, Richard AH, Pamela CC. Farmakologi: Ulasan Bergambar. Jakarta: Penerbit Widya Medika. 1995.
30. Lork S. Pemanfaatan obat tradisional dengan pertimbangan manfaat dan keamanannya. Majalah Ilmu Kefarmasian. 2006. 3(1):1-7.
31. Nor NMD, Yatim AM, Said M. Blood and urine profiles of spontaneous hypertensive rats supplemented with pink guava (Psidium guajava). Puree Sains Malaysiana. 2009. 38(6):929-34.
32. Nalwaya N, Jarald EE, Asghar S, Ahmad S. Diuretic activity of a herbal product UNEX. International Journal of Green Pharmacy. 2009. 224-6.
33. Sumardjo D. Pengantar Kimia Buku Panduan Kuliah Mahasiswa Kedokteran dan Program Strata I Fakultas Bioeksakta. Jakarta: Penerbit Buku Kedokteran EGC. 2006.
34. Depkes. Kebijakan Obat Tradisional Nasional. Jakarta: Departemen Kesehatan RI. 2007.
Published
2013-04-30
How to Cite
ANDRIYANTO, ANDRIYANTO et al. Evaluation of Diuretic Activity of Ethanolic Extract from Fruits of Belimbing Wuluh (Averrhoa bilimbi) as a Natural Diureticum: Urine Sodium and Potassium Concentrations and pH. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 11, n. 1, p. 53-59, apr. 2013. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/238>. Date accessed: 08 oct. 2024.
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