Hypoglycemic Effect of Talok Leaves Infuse in Combination with Metformin and Glibenclamide on Na2EDTA Induced-Diabetic Rats

  • GUNAWAN PAMUDJI WIDODO UNIVERSITAS SETIA BUDI
  • RINA HEROWATI UNIVERSITAS SETIA BUDI
  • BETA K. PRAMUDYAWARDHANI UNIVERSITAS SETIA BUDI

Abstract

Talok (Muntingia calabura L.) leaf is a traditional medicinal plant which empirically used to treat diabetes mellitus. This research aimed to know hypoglycemic effect of combination dose of talak leaves infuse-metformin and glibenclamide on Na2EDTA induced-diabetic rats. Diabetic animal model were conducted by induction of Na2EDTA dose of 70 mg kg bw for 3 days. The test groups divided into six groups which were treated with: 50 mg kg bw CMC-Na suspension (negative control 1st group), 0.9 mg kg bw glibenclamide (2nd group), metformin 90 mg kg bb (3rd group), 900 mg kg bw talok leaves infuse (4th group), combination of talok leaves infuse-glibenclamide (450 mg : 0.45 mg/kg bw) (5th group), combination of talok leaves infuse-metformin (450 mg : 45 mg kg bw) (6th group). The levels of blood glucose were examined in all 6 experimental groups on day 0, 4th and 7th. The data obtained were analyzed by one way ANOVA (p < 0.05). The results showed that all of combination could decrease the blood glucose levels significantly. The combination treatment of talok leaves infuse-glibenclamide showed a higher hypoglycemic effect than either drug alone, while combination treatment of talok leaves infuse-metformin showed a similar hypoglycemic effect to either drug alone.

References

1. Andrade-Cetto A, Heinrich M. Mexican plants with hypoglycemic effect used in the treatment of diabetes. Journal of Ethnopharmacology. 2005.99: 325-48.
2. Fakeye TO, Oladipupo T, Showande O, Ogunremi Y. Effects of coadministration of extract of Carica papaya Linn (family Cariaceae) on activity of two oral hypoglycemic agents. Tropical Journal of Pharmaceutical Research. 2007.6(1):671-8.
3. Aribowo D. Uji antidiabetes ekstrak etanolik 70°o daun talok (Muntingia calabura L.) pada tikus putih jantan galur wistar [Skripsi]. Surakarta: Fakultas F armasi, Universitas Setia Budi; 2009.76-7.
4. Dalimartha S. Ramuan tradisional untuk pengobatan diabetes mellitus, Cetakan IV, Jakarta: PT Penebar Swadaya; 2005. 3-48.
5. Siddiqua A, Premakumari KB, Sultana R, Vithya, Savitha. Antioxidant activity and estimation of total phenolic content of Muntingia calabura by colorimetry. International Journal of Chem Tech Research. 2010.2(1):205-8.
6. Zakaria ZA. Free radical scavenging activity of some plants available in Malaysia. Iranian Journal of Pharmacology and Therapeutics. 2007.6:87-91.
7. Shanna B, Balomajumder C, Roy P. Hypoglycemic and hypolipidemic effects of flavonoid rich extract from Eugenia jambolana seeds on streptozotocin induced diabetic rats. Food Chem Toxicol. 2008. 46(7):2376-83.
8. Ramdhani RS. Effect of Muntingia calabura L. leaves ethanol extract on blood glucose levels of aloxan induced diabetes Swiss Webster mice (Mus musculus L.) [Thesis]. Bandung: Sekolah Ilmu Teknologi Hayati Institut Teknologi Bandung; 2008.
9. Anonim. Sediaan galenik. Jakarta: Departemen Kesehatan Republik Indonesia; 1986. 4-6,8.
10. Anonim. Materia Medika Indonesia, Jilid III. Jakarta: Departemen Kesehatan Republik Indonesia; 1993. 487-9.
11. Vogel HG, Vogel WH (Eds). Drug discovery and evaluation: Pharmacological assays. Berlin: SpringerVerlag; 1997. 537-8.
12. Anonim. Buku pedoman kerja kimia klinik. Jakarta: Merck Indonesia; 1987. 62-78.
13. Kechrid Z, Amamra S, Dahdouh F, Bouzerna N. The effect of CaNa2-EDTA on metabolism of zinc and carbohydrate as well as some biochemical factors in experimental diabetes. Iranian J Publ Health. 2007. 36(1):15-21.
14. Goldberg ED, Eshchenko VA, Bovt VD. The diabetogenic and acidotropic effect of chelators. Exp Pathol. 1991.42259-64.
15. Chen JJ, Lee HH, Duh CY, Chen IS. Cytotoxic chalcones and havonoids from the leaves of Muntingia calabura. Planta Med. 2005.7 1(10):970-3.
16. Badole SL, Patel NM, Prasad A, Thakurdesai, Bodhankar SL. Interaction of aqueous extract of Pleurotus pulmonarius (Fr.) Quel-Champ. with glyburide in alloxan induced diabetic mice. eCAM. 2008.5(2)2159-64.
17. Ibrahim R. Diabetes mellitus Type II: Review of oral treatment options. International J oumal of Pharmacy and Pharmaceutical Sciences. 2010.2(1):21-30.
18. DiPiro JT, Talbert RL, Yee GC, Matzke GR, Wells BG, Posey LM (Eds.). Pharmacotherapy: A pathophysiologic approach, 7th Ed. New York : McGraw-Hill; 2008. 1223-4.
Published
2012-09-30
How to Cite
WIDODO, GUNAWAN PAMUDJI; HEROWATI, RINA; PRAMUDYAWARDHANI, BETA K.. Hypoglycemic Effect of Talok Leaves Infuse in Combination with Metformin and Glibenclamide on Na2EDTA Induced-Diabetic Rats. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 10, n. 2, p. 93-98, sep. 2012. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/254>. Date accessed: 05 nov. 2024.
Citation Formats
Issue
Vol 10 No 2 (2012): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.