Antioxidant Activity Test and Determination of Phenolic and Flavonoid Contents from Pandanus conoideus Lam.

  • NI MADE DWI SANDHIUTAMI UNIVERSITAS PANCASILA
  • A.A WIWIEK INDRAYANI UNIVERSITAS UDAYANA

Abstract

Antioxidants are compounds which have ability to inhibit free radical reactions in the human body. Objectives of this research are to evaluate the antioxidant activities of extract and fractions from Pandanus canoideus Lam. fruits, to determine total phenolic and total flavonoid contents, and to correlate the total phenolic and total flavonoid contents of those fractions with their antioxidant activities. The Pandanus conoideus Lam. fruits or locally known as “buah merah” were extracted by methanol, then evaporated and dissolved in aquadest. This solution is subsequently partitioned using chloroform and ethyl acetate to obtain chloroform, ethyl acetate, and water fractions. The antioxidant activities were determined by radical scavenging assay using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical. The total phenolic and total flavonoid contents were determined by mean of spectrophotometry. The results showed that fractions of water, ethyl acetate, and chloroform revealed antioxidant activities with IC50 values of 399.06, 10.41, 1416.68 µg/mL respectively, whereas the methanolic extract of Pandanus conoideus Lam. fruits before partition has IC50 of 186.31 µg/mL. The antioxidant activities of these extract and fractions are lower than that of vitamin E (IC50 8.27 µg/ml). A linier positive relationship existed between the total phenolic contents of these extract and fractions with their antioxidant activities as shown by equation of y = 13.085x + 754.91; r2 = 0,3641, while the correlation between the total flavonoid contents and their antioxidant activities revealed a linier regression of y = -282.9x + 868.05; r2 = 0.4484.

References

1. Supriadi,dkk. Tumbuhan Obat Indonesia: Penggunaan dan Khasiatnya. Edisi 1 . Jakarta: Pustaka Populer Obor; 2001. 11-4.

2. Departemen Kesehatan RI. Pedoman Pelaksanaan Uji Klinik Obat Tradisional. Edisi 1. Jakarta; 2000.1-22.

3. Budi M, dan Paimin FR. Buah Merah. Jakarta: Penebar Swadaya; 2004. 3-26, 47-56, 67-8.

4. Pokorni J, Yanishlieva N, and Gordon M. Antioxidant in Food: Practical Applications. New York: RC Press; 2001.

5. Brigelius-Flohe R, Traber MG. Vitamin E : Function and Metabolism. The FASEB Journal. 1999. 13: 1145-55.

6. Prior RL, Wu X and Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 2005. 55:2698A-J.

7. Chun OK, Kim DO, and Lee CY. Superoxide Radical Scavenging Activity of The Maj or Polyphenols in Fresh Plums. J Agric Food Chem. 2003.51:8067-72.

8. Zou Y, Lu Y and Wei D. Antioxidant activity of Flavonoid-rich extract of Hypericum perforatum L in vitro.J Agric Food Chem. 2004. 52:5032-9.

9. Mongkolsilp M, Pongbupakit I, Sae-Lee N, and Sitthithaworn W. Radical scavenging activity and total phenolic content of medicinal plants used in primary health care. SWU. J Pharm. Sci. 2004. 932-5.

10. Javanmardi J, Stushnoff C, Locke E, and Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. J. Food Chem. 2003. 83:547-50.

11. Kahkonen MP, HopiaAI,Vuore1a HJ, Rauha JP, Pihlaja K, Kujala TS, and Heinonen M. Antioxidant activity of extracts containing phenolic compounds. J Agric Food Chem.1999. 47:3954-62.

12. Amic DD, Beslo D, and Trinajstic. Structure-radical scavenging activity relationship of flavonoids. Croatia Chem Acta. 2003.76 (1):55-61.

13. Hudson BJF.Food antioxidants. London and New York Elsevier Applied Science. 990: 1-8.
Published
2012-04-30
How to Cite
SANDHIUTAMI, NI MADE DWI; INDRAYANI, A.A WIWIEK. Antioxidant Activity Test and Determination of Phenolic and Flavonoid Contents from Pandanus conoideus Lam.. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 10, n. 1, p. 13-19, apr. 2012. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/271>. Date accessed: 19 may 2024.
Section
Articles