Production of Beta-Glucan from Saccharomyces cerevisiae and Antioxidant Activity on t-BHP Induced Red Blood Cells

  • KUSMIATI KUSMIATI LEMBAGA ILMU PENGETAHUAN INDONESIA
  • NI WAYAN S. AGUSTINI LEMBAGA ILMU PENGETAHUAN INDONESIA
  • SWASONO R. TAMAT UNIVERSITAS PANCASILA
  • BAYU RIANTO UNIVERSITAS PANCASILA

Abstract

Beta glucan is a polysaccharide that can be found in cereals, yeast, bacteria, and algae. Beta glucan produced by Saccharomyces cerevisiae has the ability to enhance the body’s defense system. The study aims to investigate the antioxidant activity of B glucan produced by three strains of Saccharomyces sp BR 1, BR 2,and SC. Antioxidant activity assay was carried out in vitro on t BHP induced red blood cells (REC) by observing the level of catalase activity, SOD activity and MDA levels as lipid peroxidation product. The investigation was performed on 6 groups: normal control, negative control, positive control (RBC+ t-BHP + vitamin E 100 IU), and samples (RBC+t-BHP) treated with 1.4 µg/mL β-glucan from Saccharomyces strain BR1, BR2 and SC respectively. Saccharomyces cerevisiae strain SC produced the highest β-glucan of 88.70% (glucose equivalent), while the BR1 and BR2 of 22.56% and 46.28% respectively. Antioxidant activity assay showed that the highest increase in catalase activity (581.48%) was given by the B glucan from SC strain, the highest increase of SOD activity and decrease of MDA levels were given by the β- glucan from BR1 strain (359.17% and 48.18%) as compared to negative controls. Statistical test (α = 0.05) showed antioxidant activity of β-glucan from Saccharomyces Sp BR2 and SC did not differ significantly from the activity of vitamin E (100 IU).

References

1. James MJ. Modern Food Microbiology. Edition 5. New York: Chapman-Hall; 1992. 38-40.

2. http://www.pdrhealth.com. Yeast Beta-D-glucan. Accessed January 28, 2007.

3. Manfredini V, Roehrs R, Peralba, Henriques JAP, Saffi J, Ramos ALLP. Glutathione peroxidase Protects Saccharomyces cerevisiae sod1 sod2 double mutants against oxidative damage. Braz J Med Res. 2004. 37(2):159-65.

4. Jong P, Chris MG, Davies MJ, Dawes IA. The cytoplasmic Cu, Zn superoxide dismutase of Saccharomyces cerevisiae in required for freeze thaw resistant to stress. JBiol Chem. 1998. 273(36):22921 28.

5. Yangkova PV, Vassileva TR, Kujumdzieva A. Catalase enzyme in mitochondria of Saccharomyces cerevisiae. EJB Electronic Journal of Biotechnology. 2002. 5(1).

6. Cunnif P. Official methods of analysis of AOAC International. 16th ed. Chapter 32. Arlington: AOAC International Publishing; 1995. 30.

7. Achuba I F idelis. Effect of vitamins C and E intake on blood lipid concentration, lipid peroxidation, superoxide dismutase and catalase activities in petroleum contaminated diet fed rabbit. European Journal of Scientific Research. 2005. 12(1):1-8.

8. Measurement of total malondialdehyde in plasma and tissues by HPLC and TBA assay. Experimental Research. 2006. 11(2): 88-92.

9. www.worthington-biochem.com. Superoxide dismutase. Catalase. Accessed June 26, 2006.
Published
2012-04-30
How to Cite
KUSMIATI, KUSMIATI et al. Production of Beta-Glucan from Saccharomyces cerevisiae and Antioxidant Activity on t-BHP Induced Red Blood Cells. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 10, n. 1, p. 44-49, apr. 2012. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/276>. Date accessed: 26 apr. 2024.
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Vol 10 No 1 (2012): JIFI
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