The Influence of Pasak Bumi’s Root Standardized Extract towards Testes Histopathology and TestosteroneLevel in Rats

  • FARIDA HAYATI UNIVERSITAS GADJAH MADA
  • SITARINA WIDYARINI UNIVERSITAS GADJAH MADA
  • LUKMAN HAKIM UNIVERSITAS GADJAH MADA
  • NGATIDJAN NGATIDJAN UNIVERSITAS GADJAH MADA
  • MUSTOFA MUSTOFA UNIVERSITAS GADJAH MADA

Abstract

The root of pasak bumi (Eurycoma longifolia, Jack) is one of plant from Indonesia known as aphrodisiac. This study was conducted to evaluate the influence of pasak bumi standardized extract to testosterone level and histopathological changes of the testes in male Wistar rats. Sample were 50 adult male rats aged 3 4 months were divided into 5 groups. Group I (control) was given distilled water, group II (positive control), was given testosterone (Andriol ®), group III, IV, and V were each given a standardized extract of the roots of pasak bumi doses of 50, 100, and 200 mg/kg of body weight, respectively. The extract was given orally twice a day for six days and forty nine days and then testes was taken out on 7th and 50th day. Testosterone level was assayed on 7th and 50th day by the ELISA methods. The results were analyzed using one way ANOVA, and post hoc Dunnet (2-sided) (p < 0.05). The data of this study shows that treatment with water extract of pasak bumi root influence the number of Leydig cells that would also increase the concentration of testosterone in Wistar male rats positively at a dose of 100 mg/kg and 200 mg/kg body weight.

References

1. Yakubu MT, Akanji MA and Oladiji AT. Male sexual dysfunction and methods used in assessing medicinal plants with aphrodisiac potentials. Pharmacog Rev 1. 2007. 49-56.

2. Wibowo S dan Gofir A. Disfungsi ereksi. Yogyakarta: Pustaka Cendekia Press; 2007. 5-8.

3. BaziadA. Menopause dan Andropause. Jakarta: Yayasan Bina Pustaka Sarwono Prawirohardjo; 2003. 217-19.

4. Ang HH, Sim MK. Eurycoma longifolia, Jack enhances libido in sexually experienced male rats. Exp Anim. 1997. 46(4):287-90.

5. Ang HH, Sim MK. Eurycoma longifolia, Jack and orientation activities in sexually experienced male rats, Biol Pharm Bull. 1998a. 21(2):153-5.

6. Ang HH, Sim MK. Eurycoma longi olia, Jack increases sexual motivation in sexually naive male rats. Arch Pharm res. 1998b. 21(6):779 81.

7. Ang HH, Ikeda S, Gan BK. Evaluation of potency activity of aphrodisiac in Eurycoma longifolia, Jack. Phytother Res. 2001. 15(5):435-6.

8. Ang HH, Lee KL. Effect of Eurycoma longifolia, Jack on libido in midlle aged male rats. J Basic Clin Physiol Pharmacol. 2002. 13(3):249-54.

9. Ang HH, Ngai TH, Tan TH. Effect of Eurycoma longifolia, Jack on sexual qualities in middle aged male rats. Phytomed. 2003a. 10(6-8):590-3.

10. Ang HH, Lee KL, Kiyoshi M. Eurjycoma longifolia, Jack enhances sexual motivation in middle aged male rats. J Basic Clin Physiol Pharmacol. 2003b. 14(3):301-8.

11. Sandroni P. Aphrodisiacs past and present: a Historical Review. Clin Autonom Res . 2001. 11:303-7.

12. Rumiati F. Pengaruh pemberian Solasodin per Oral terhadap gambaran sel-sel leydig dan kadar testosteron darah mencit (Mus Muculus, L) jantan dewasa. Tesis. Yogyakarta: Fakultas Kedokteran Universitas Gadjah Mada; 1999.

13. Kurniawan C. Sinopsis Fisiologi. Yogyakarta: Pidi Publisher; 2006. 228.

14. Christensen and Mori HAK. Morphometric analysis of leydig cells in the normal rat testis. The Journal of Cell Biology 1980 .(84):340-54.

15. Winarni D. Efek ekstrak akar ginseng jawa dan korea terhadap libido mencit jantan pada prakondisi testosteron rendah. Berk Penel Hayati . 2007. 12:153-9.

16. Nainggolan 0 dan Simanjuntak JW. Pengaruh Ekstrak Etanol Akar Pasak Bumi (Eurycoma longifolia, Jack.) Terhadap Perilaku Seksual Mencit Putih. Cermin Dunia Kedokteran. 2005. 146:55-7.

17. Cunningham J G. Textbook of Veterinary Physiology, Third Edition, Philadelphia: W. B. Saunders Company. 2002. 421-7.

18. Ganong WP. Buku Aj ar Fisiologi Kedokteran diterjemahkan oleh H.M Djauhari W. Edisi 20. Jakarta: Penerbit Buku Kedokteran EGC; 2001.408.

19. Junqueira LC. Camero JC,.Ke]ly R0. Histologi dasar. Edisi 8. Jakarta: Penerbit buku kedokteran EGC;1998. 419-29.
Published
2012-04-30
How to Cite
HAYATI, FARIDA et al. The Influence of Pasak Bumi’s Root Standardized Extract towards Testes Histopathology and TestosteroneLevel in Rats. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 10, n. 1, p. 50-56, apr. 2012. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/277>. Date accessed: 23 nov. 2024.
Citation Formats
Issue
Vol 10 No 1 (2012): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.