Isolation and Characterization of Antibacterial Compound Against Streptococcus mutans from Ethanol Extract of Kalanchoe pinnata (Lamk.) Pers. Leavcs
Abstract
Streptococcus mutans is one of the microbes which is responsible for the cause of bad breath. The activity of Kalanchoe pinnata (Lamk.) Pers. leaves ethanol extract is four times stronger than the water extract in inhibiting the growth of Streptococcus mutans. The aim of research is to isolate and characterize an antibacterial compound of ethanol extract of Kalanchoe pinnata (Lamk.) Pers. leaves. The extract was fractionated using column chromatography. The fractions were purified by TLC and the isolate were tested for its purity using HPLC. Isolate was characterized using UV-Vis spectrophotometry and FTIR. Fractionation by column chromatography generated 9 fractions. The fraction VII have the highest diameter of inhibitory against Streptococcus mutans. The yield of isolate was 9.18% of the fraction VII or 0.84% of the ethanol extract with purity of 95%. Isolate was suspected as a group of saponin compound.
References
2. Firdaus, Kartiningsih, Yantih N. Minimum inhibitory concentration (MIC) of powder ethanol extracts of kalanchoe pinnata against sterptococcus mutans. Proceeding of the third international conference on matematics and natural science. 2010. 520-3.
3. Akinsulire OR, Aibinu IE, Adenipekun T, Adelowotan T, Odugbemi T. In vitro antimicrobial activity of crude extracts from plants Bryophyllum pinnatum and Kalanchoe crenata. African Journal of Traditional, Complementary and Alternatif Medicines. 2007. 4(3): 338-44.
4. Akinpelu D.A. Antimicrobial activity of Bryophyllum pinnatum leaves. Fitoterapia. 2000. 71(2):193-4.
5. Yantih N, Diah W, Anggelina O,Firdaus, and Kartiningsih. General standard parameters of aqueous and ethanol extracts of Kalanchoe pinnata. Proceeding the third International Conference on Mathematics and Natural Science. 2010. 558-63.
6. Okwu DE, Nnamdi FU. TWO novel flavonoids from Bryophyllum pinnatum and their antimicrobial activity. J Chem Pharm Res ISSN No:0975-7384 CODEN(USA): JCPRC5.2011.3(2):1-10
7. Famsworth NR. Biology and phytochemical screening of plants. Pharm Sci. 1966. 55: 225-76.
8. Silverstein RM, Webster FX, Kiemle DJ . Spectrometric identification of organic compounds. New York: John Wiley & Sons Inc; 2005. 72-108, 119-123.
Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.
This licence applies to Author(s) and Public Reader means that the users mays :
- SHARE:
copy and redistribute the article in any medium or format - ADAPT:
remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication) - ALIKE:
If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original. - NO ADDITIONAL RESTRICTIONS:
You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
It does however mean that when you use it you must:
- ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
You may not:
- NONCOMMERCIAL: You may not use the article for commercial purposes.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.