The Acceleration of Wound Healing of Snakehead Fish Cream towards Rabbit’s Skin Wound Histopathologically
The aims of the study were to investigate the effect of cream containing various concentrations of snakehead fish on the duration of wound recovery and skin histology as compared to control. Samples of the study were nine rabbits divided into three groups for treatment, i.e. group I (0.5%), group II (1%), and group III (2%). Each rabbit in each group was wounded with scalpel as wide as 4 cm2 on the left and right dorsal thigh. Wound on the left dorsal was applied with snakehead fish cream (accordingly to each concentration) and the right dorsal with basic cream as control. The observations were carried out on day 3, day 6 and day 12 by measuring the wound size and taking picture of the wounded area. On the day 12, skin incision were performed on each group to observe histopathology. The data were analyzed statistically by the SPSS 15 program of repeated measures test. The results indicated that group III (2%) had significant effect on wound recovery by decreasing the wound size every day (p < 0.05). The results were confirmed by observing the skin histopathology, indicating that cream containing 2% snakehead fish could faster improve the tissue granulation as compared to the 0,5% and 1% concentration. It can be concluded that the application of 2% snakehead fish cream for 12 days can accelerate the wound recovery.
2. Manan A, Haruan A. “Fresh Water” Wound Healer.Malaysia: University of Putra Malaysia; 2006.
3. Baie SH , Sheikh KA . The wound healing properties of Channa striatus-cetrimide cream – tensile strength measurement. J Ethno-Phamacol 2000b; 71: 93-100.
4. Taslim AN , Hadju V, Attamimi F, Tawali A, Saifuddin S. The Report of Snakehead Fish Research. Food, Nutrient, and Health Research Center. Makassar: Hasanuddin University; 2005.
5. Ode S. The Giving Influence of the Snakehead Fish capsule on Albumin Level and nutrient status of ODHA Patient in Wahidin Sudirohusodo Hospital. The Postgraduate Program. Makassar: Hasanuddin University. Makassar; 2007.
6. Direktorat Jenderal Pengawasan Obat dan Makanan. Farmakope Indonesia. Edisi IV . Jakarta: Departemen Kesehatan Republik Indonesia; 1995.
7. Raghow R. The role of extracelular matrix in postinflamatory wound healing and fibrosis. FASEB J. 2000. 8: 823-31.
8. Declair V. The importance of growth factors in wound healing. Ostomy/ Wound Management. 2000; 45: 64-72.
9. Romero S, Peixoto, Christina. The Effect of Topical Application of Sunflower–Seed Oil on Open Wound Healing in Lambs. Journal Medicine. 2004. 19(3): 196-209.
10. Martin P and Leibovich. Inflammatory cells during wound repair the good, the bad and the ugly. Trends in Cell Biology. 2005. 15(11), 599-607.
11. Montgomery DC. Design and Analysis of Experiments. The Fifth Edition. Arizona State University. John Wiley Jurnal Ilmu Kefarmasian Indonesia
4. Percepatan_Robert t.indd 6 11/21/2011 11:17:33 Pm Vol 9, 2011 Jurnal Ilmu Kefarmasian Indonesia 97 & Sons Inc. New York; 2001.
12. Baie SH , Sheikh KA . The wound healing properties of Channa striatus-cetrimide cream-wound contraction and glycosaminoglycan measurement. J Ethno-Phamacol. 2000a; 73:15-30.
13. Santoro MM and Gandino G. Cellular and Molecular Facets of Keratinocyte Reepithelization during Wound Healing. Experimental Cell Research. 2005. 304(1), 274-86.
14. Effendi C, Wilfred M. Bailey’s Textbook of Histology. College of Physicians & Surgeons. Columbia University. The fifteenth Edition. The Williams & Wilkins Company. USA ; 2003. 15. Datta HS , Wound Healing Activity of Topical Application Forms Based On Ayurveda. The Oxford Journal. eCAM Advance Access Published. 2009. 1-10.
16. Cavallo J. The Study of Amino Acids, Albumin, Zinc Mineral Profiles on snakehead fish (Ophiocephalus streatus) and Tomang fish. Malang: Faculty of Fishery. Brawijaya University; 1998.
Copyright @2017. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted non-commercial used, distribution and reproduction in any medium
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.