Inhibitory Activity Test of Quercitrin Isolate from Masisin Parasite Dendrophthoe pentandra (L.) Miq. Against L1210 Leukemia Cells

  • ERMIN KATRIN W. LABORATORIUM BAHAN KESEHATAN PATIR-BATAN
  • RISMA MARISI TAMBUNAN UNIVERSITAS PANCASILA
  • HENDIG WINARNO LABORATORIUM BAHAN KESEHATAN PATIR-BATAN

Abstract

Parasitic plant [Dendrophthoe pentandra (L.) Miq.] on the masisin [Rhodomyrtus tomentosa (Aiton) Hassk.] was traditionally used for cancer treatment in Central Kalimantan. Maceration of dried Dendrophthoe pentandra using ethanol followed by ethyl acetate produced ethanol and ethyl acetate extracts which exhibit inhibitory activity against leukemia L1210 cells with IC50 of 17.3 and 20.9 µg/mL, respectively. Fractionation of ethyl acetate extract by column chromatography yielded 7 fractions (F1~F7). Inhibitory activity test on fractions F1~F7 against leukemia L1210 cells showed inhibitory activity with IC50 0f 19.9, 19.0, 20.7, 16.0, 13.7, 12.5, and 17.4 µg/mL, respectively. During evaporation of F5 by rotary evaporator, a yellowish precipitate was observed and identified as quercitrin (quercetin 3-O-rhamnoside). Inhibitory activity test on quercitrin against leukemia L1210 cell showed that the crystal has an IC50 of 14.7 µg/mL. Separation of F7 by semipreparative HPLC reverse phase column yielded isolate A and B which exhibited inhibitory activity on leukemia L1210 cells with IC50 of 15.0 and 14.4 µg/mL, respectively. Subsequent solvent partition of ethanol extract into ethyl acetate-water and separation of ethyl acetatesoluble portion by column chromatography produced 7 fractions (F8~F14). Further separation of F14 by column chromatography yielded quercitrin and isolate C which has not been identified yet.

References

1. Lubis NL dan Hasnida. Dukungan sosial pada kanker, perlukah. Medan: USU Press; 2009. 2. diambil dari: http://usupress.usu.ac.id/files/Final_Normal_Web.pdf. diakses pada 29 Oktober 2010.

2. Mizuno M and Yoneda K. Folk medicines and kanpo: Usage of medicinal plants. Japan: Shin-Nihon Hohki Pub.; 1997. 518-9. (in Japanese).

3. Weiβ RF. Phytotherapy. Tokyo: Yasaka Shobo Pub.; 1995. 405-6. (In Japanese).

4. Cheng, RKYZ. Anticancer research on Loranthaceae plants, Drugs of the Future. 1997. 22: 519-30.

5. Ohashi K, Winarno H, Mukai M, Inoue M, Prana SM, Simanjuntak P, Shibuya H. Indonesian medicinal plants XXV: Cancer cell invasion inhibitory effects of chemical constituents in the parasitic plant Scurrula atropurpurea (Loranthaceae). Chem Pharm Bull. 2003. 51 (3): 489-92.

6. Winarno H. Antiproliferative activity of octadeca 8,10,12 triynoic acid against human cancer cell lines, Berita Biologi. 2009. 9(4):343-8.

7. Perry and Lily M. Medicinal plants of East and Southeast Asia: Attributed properties and uses. Massachusetts. The MIT Press; 1980. 265.

8. Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci. 1966. 225-65.

9. Anonim. Materia medika Indonesia. Jilid III. Jakarta: Direktorat J enderal Pengawasan Obat dan MakananDepartemen Kesehatan Republik Indonesia; 1995. 155-9.

10. Markham KR and Ternai B. C-13 NMR of flavonoids II: Flavonoids other than ilavones and flavonol aglycones. Tetrahedron. 1976. 32:2607-12.

11. Winarno H. Chemical study on Indonesian parasitic plants Scurrula atropurpurea and S. usca (Loranthaceae); Ph.D Dissertation. Japan, Fukuyama University; 2003. 8-12.

12. Swanson SM and Pezzuto JM. Bioscreening tehnique for cytotoxic potential and ability to inhibit macromolecule biosynthesis. In: Thompson EB. editor. Drug bioscreening. New York: John Wiley & Sons; 1990. 273-97.
Published
2011-04-30
How to Cite
W., ERMIN KATRIN; TAMBUNAN, RISMA MARISI; WINARNO, HENDIG. Inhibitory Activity Test of Quercitrin Isolate from Masisin Parasite Dendrophthoe pentandra (L.) Miq. Against L1210 Leukemia Cells. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 9, n. 1, p. 1-7, apr. 2011. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/311>. Date accessed: 22 aug. 2019.
Section
Articles