Protein Profile of Klebsiella pneumonia K3 After Inactivation by Gamma Rays and Heat at 65 C



Klebsiella pneumoniae is one of coliform bacteria which causes human and mammalian diseases. The bacteria dominate in dairy cow milk which has been infected by mastitis and has resistent on antibiotic. Vaccination is one of aims to prevent the diseases. Nuclear technique could be used to have a vaccine candidate.This research was conducted to get inactivated K. pneumoniae by gamma irradiation and heat inactivated as vaccine candidate. The experiments were done by determination of inactivated doses, protein content, and protein profile. analysis. K. pneumoniae could be inactivated using gamma rays by doses higher than 600 Gy. Neither irradiation nor heat (65 oC) influenced the K. pneumoniae total protein content The intensity of protein profile in gamma ray inactivated was higher than heat inactivated. There were 35, 36, and 60 kDa protein which were diagnosed as antigen protein. It could be concluded that inactivated by gamma irradiation K. pneumoniae could be chosen as a vaccine candidate and as a model for other bacterial vaccine.


1. IAEA. Combating infection in developing countries. Vienna, Austria; 2000.

2. Machi S. Nuclear techniques serve mankind. Japan Atomic Industrial Forum (JAIF) Inc; 2002.

3. IAEA. Animal health: Global support for diagnosing infectious diseases. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture. IAEA Bulletin. 2010.

4. Bandyopadhyay SM and Mandal C. Targeting glycoproteins or glycolipids and their metabolic pathways for antiparasite therapy. Adv Exp Med Biol. 2008. 625:87-102.

5. Rachmilewitz D.Toll-like receptor 9 signaling mediates the anti-inflammatory effects of probiotics in murine experimental colitis. Gastroenterology. 2004. 520-28.

6. Datta SK. Vaccination with irradiated Listeria induced protective T cell immunity. H Immunity J Immuni. 2006. 5-13.

7. Bahri S. dan Sugoro I. lsolasi dan identifikasi bakteri coliform pada susu sapi perah yang terinfeksi mastitis. Jumal Biologi dan Lingkungan Al-Kaunyah. F ST-UIN Syahid. Jakarta. 2007 .

8. Luki S. Sensitivitas Antibiotik bakteri coliform penyebab penyakit mastitis [skripsi]. Depok: ISTN; 2009.

9.Ruegg P. Evaluating the effectiveness of mastitis vaccines. Madison: University of Wisconsin; 2001.

10. Sugoro I, dan Hermanto S. Penentuan dosis inaktif bakteri Escherichia coli hasil irradiasi gamma. Prosiding Seminar Nasional KKL, PTKMR BATAN, 2008.

11. Ikmalia, Hermanto S, Sugoro I. Profil protein Escherichia coli hasil inaktivasi sinar gamma. Prosiding Seminar Nasional Biokimia, UI, Jakarta, 2008.

12. Sanakkayala N, Okolovska A, Gulain J, Hogenesch H. Sriranthan N, Boyle S, Schurig G, Vemuilapalli R. Induction of antigen-speciiic Thl-type Immune responses by gamma-irradiated recombinant Brucella abortus RB51. Clinical and diagnostic laboratory immunology. American Society for Microbiology. 2005.

13. Nuraeni N. Profil protein Yersinia enterocolitica hasil inaktivasi sinar gamma [skripsi]. Jakarta: Prodi Biologi UIN Syahid; 2008.

14. Alatas Z. Efek paparan radiasi pada manusia. Jakarta: Pusat Penelitian dan Pengembangan Keselamatan Radiasi dan Biomedika Nuklir. Badan Tenaga Nuklir. Nasional (BATAN); 2005.

15. Tetriana D, dan I Sugoro. Peran teknik nuklir dalam pembuatan vaksin. Buletin ALARA vol 2. BATAN. 2009.
How to Cite
SUGORO, I.; DJAJANEGARA, I.. Protein Profile of Klebsiella pneumonia K3 After Inactivation by Gamma Rays and Heat at 65 C. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 9, n. 1, p. 14-19, apr. 2011. ISSN 2614-6495. Available at: <>. Date accessed: 25 feb. 2024.