Water Extract of Ling Zhi (Ganoderma lucidum (Leysser) Karsten) Increases the Percentage of Relative CD8+ T Lymphocyte Cell in Rat Induced by Doxorubicin

  • MUTHI IKAWATI UNIVERSITAS GADJAH MADA
  • ANNISA KARAMITA UNIVERSITAS GADJAH MADA
  • EDIATI EDIATI UNIVERSITAS GADJAH MADA
  • RATNA ASMAH SUSIDARTI UNIVERSITAS GADJAH MADA

Abstract

Cancer therapy using chemotherapeutic agents associated with many adversed side effects, including immunosupressant. The use of immunostimulator together with chemotherapeutic agent (co-chemotherapy) can be the alternative method to solve that problem. Ganoderma lucidum (Ling Zhi) has been reported to have immunostimulatory activity. The aim of this research was to evaluate the immunostimulatory activity of water extract of G. Zucidum by determining the relative CD8+ T lymphocyte cell percentage in rats induced by doxorubicin. Extraction of plant material was carried out by infusion method. Sprague Dawley female rats were divided into six groups, they were doxorubicin as control, commercial product as comparing control, 100 mg/kgBW and 450 mg/kgBW extract treatment, extract control, and without treatment control. Relative CD8+ T lymphocyte cell percentages of blood samples were obtained by flow cytometry by using Multiset program. The data were analyzed statistically using paired sample t test and one way ANOVA continued by Post Hoc test. The result showed that the water extract of G. lucidum increased the relative CD8+ T lymphocyte cell percentage in rats induced by doxorubicin. The water extract of G. lucidum is promising to be developed as co-chemotherapy immunostimulatory agent.

References

1. Brundtland GH. Global cancer rates could increase by 5000 to 15 million by 2020; 2003. diambil dari WWW. who.int. diakses 29 November, 2010.

2. Patel D, Shukla S, Gupta. Apigenin and cancer chemoprevention: progress, potensial, promise (Review). Int J Oncol. 2007. 30:233-45.

3. Zhang X, Li WG, Wu YJ, Gao MT. Amelioration of doxorubicin induced myocardial oxidative stress and immunosuppression by grape seed proanthocyanidins in tumor bearing mice. J Pharm Pharmacol. 2005. 57(8):1043-51.

4. Sliva D. Ganoderma lucidum in cancer research. Leukemia Research. 2006. 30:767-8.

5. Lin Z. Cellular and molecular mechanism of immunomodulation by Ganoderma lucidum. J Pharmacol Sci. 2005. 992144-53.

6. Pillai TG, Salvi VP, Maurya DK, Nair CKK, J anardhanan. Prevention of radiation-induced damages by aqueous extract of Ganoderma lucidum occurring in southern parts of India. Current Science. 2006. 91(3):341-4.

7. Anonim. MlMS Indonesia Petunjuk Konsultasi. Edisi 7. Jakarta: PT. Info Master; 2008. 252.

8. Ikawati I, Fitria M, Fauzi IG. Ekstrak etanolik daun awar-awar (Ficus septica Burm. F.) sebagai imunomodulator pada tikus galur Sprague Dawley yang dipej ani doxorubicin [laporan penelitian PKM-P]. Yogyakarta: Fakultas Farmasi Universitas Gadjah Mada; 2009.

9. Anonim. Ganoderma lucidum’s anti cancer properties investigated; 2003. diambil dari http://www. thehealthierlife.co.uk. diakses 29 November, 2010.

10. Liang JB, Chen X, Wang J, Cheng B. HPCE determination of monosaccharide composition of Ganoderma lucidum polysaccharides [abstract]. Chin J Pharm Anal. 2008. diambil dari http://en.cnki.com. cn/Article_en/CJFDTOTAL-YWFX2008 l 1022.htm. diakses 29 November, 2010.

11. Anonim. Determination of reducing sugar by LuffSchoorl’s methods. 2002. diambil dari http://WWW. starchdk/isi methods/28luffhtm. diakses 29 November, 2010.

12. Anonim. CD8. 2009. diambil dari www.wikipedia.com. diakses 29 November, 2010.

13. Anonim, T Cell. 2009. diakses dari www.wikipedia.com. diakses 29 November, 2010.

14. Gabriel JA. The biology of cancer. 2nd ed. England: John Wiley & Sons LTD; 2007.

15. Lin ZB, Zhang HN. Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its possible nechanism [review]. Acta Pharmacol Sin. 2004. 35(11):1387-95.

16. Wang YY, Khoo KH, Chen CC, Wong CH, Lin CH, Ihen HS, et al. Immune-modulating antitumor activities f Ganoderma lucidum (Reishi) polysaccharides. US atent 7135183. 2006.
Published
2011-04-30
How to Cite
IKAWATI, MUTHI et al. Water Extract of Ling Zhi (Ganoderma lucidum (Leysser) Karsten) Increases the Percentage of Relative CD8+ T Lymphocyte Cell in Rat Induced by Doxorubicin. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 9, n. 1, p. 20-26, apr. 2011. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/317>. Date accessed: 22 july 2024.
Section
Articles